Trichosanthes�kirilowii extract enhances repair of UVB radiation‑induced DNA damage by regulating BMAL1 and miR‑142‑3p in human keratinocytes

Abstract

Ultraviolet B (UVB) radiation induces DNA damage, oxidative stress and inflammation, and suppresses the immune system in the skin, which collectively contribute to skin aging and carcinogenesis. The DNA damage response, including DNA repair, can be regulated by the circadian clock and microRNA (miRNA) expression. The aim of the present study was to evaluate the reparative action of Trichosanthes kirilowii extract (TKE) against UVB irradiation-induced DNA damage in human keratinocytes. TKE demonstrated low cytotoxicity in normal HaCaT keratinocytes at low doses (up to 100 µg/ml). The results of a comet assay revealed that TKE enhanced the repair of UVB-induced DNA damage. TKE significantly upregulated the expression of the core clock protein, brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein-1 (BMAL1), and downregulated the expression of miRNA (miR)-142-3p, as demonstrated using western blotting and the reverse transcription-quantitative polymerase chain reaction. Furthermore, the suppression of miR-142-3p by a specific inhibitor positively correlated with the repair activity. Overall, the data obtained demonstrated that TKE enhanced the repair of UVB-induced DNA damage by regulating the expression of BMAL1 and miR-142-3p. Consequently, TKE can be considered a potential candidate for the treatment of skin diseases associated with UVB-induced damage.

Clinical efficacy of herbal extract cream on the skin hydration, elasticity, thickness, and dermis density for aged skin: A randomized controlled double‐blind study

“…This leads to antioxidative effects in human skin. Furthermore, previous studies reported that the main plants ingredients in SHYBE exhibit various anti‐aging properties . The aqueous root extract Asparagus cochinchinensis showed antioxidant abilities as strong as vitamin C .…”

“…Astragalus membranceus increased hyaluronic acid content and cellular expression of telomerase reverse transcriptase . Trichosanthes kirilowii extract enhanced repair of ultraviolet radiation‑induced DNA damage by regulating BMAL1 and miR‑142‑3p in human keratinocytes . Glycyrrhiza glabra induced antioxidant enzymes and increased TNF‐γ, IL‐6, IL‐Iβ, NF‐kB, testosterone, and DHEA‐S levels …”

<p>Triptolide Inhibits the Proliferation of HaCaT Cells Induced by IL22 via Upregulating miR-181b-5p</p>

“…Recent study indicated that trichosanthes kirilowii, a traditional Chinese herb, exerted a protective role for the treatment of skin diseases via regulating the level of miR-142. 31 Previous studies indicated that some miRNAs play a vital role in the development of psoriasis, such as miR-125b-5p, miR-181b-5p, miR-187, miR-223, and miR- 744. 3,19,21,22 Our data indicated that triptolide had very limited effect on the levels of miR-125b-5p, miR-187, miR-223, and miR-744 in HaCaT cells.…”

Effect of oocyte vitrification on DNA damage in metaphase II oocytes and the resulting preimplantation embryos

“…The data are representative of three or more independent experiments. **p < .01, ***p < .001, ****p < .0001 [Color figure can be viewed at wileyonlinelibrary.com] DNA damage can be induced by endogenous metabolites that can react with macromolecules, such as lipids, proteins or nucleic acids, and exogenous stimulants, such as ionizing radiation, ultraviolet radiation, and ROS (Babazadeh et al, 2012;Joo, Hong, Kim, & Choi, 2018;Kimeswenger et al, 2016;Salmon et al, 2004). It was found that vitrification increased ROS levels, which is consistent with our results that showed that oocyte vitrification enhanced ROS generation in MII oocytes and resulted in zygotes and two-cell embryos.…”