Fifty five sexual partners of women with positive cultivation finding of Tvaginalis were also examined. Specimens from the distal urethra and the sediment of the morning urine were examined by microscopy, culture and DIBA.
Sample collectionThe material from the vagina and urethra for wet mount was taken with bacteriological loop to a drop of sterile saline on a microscope slide and evaluated immediately for the presence of motile parasites by phase contrast microscopy at x 200 magnification. Urine sediment examination was performed the same way. For cultivation, swabs from the vagina and urethra were collected directly into 10 ml of modified Diamond's medium TYM"2 supplemented with 10% bovine serum, 1000 IU/mI penicillin and 1 mg/ml streptomycin. Culture tubes were examined microscopically for motile parasites after 24 and 72 hour incubation at 37°C.