Trichomonads from Poikilotherms: Nutritional and Physiological Notes*

Lee, John J.; Pierce, Stanley; Hutner, S. H.; Smith, Barry; Gurski, Dennis R.

doi:10.1111/j.1550-7408.1962.tb02651.x

Cited by 10 publications

(5 citation statements)

References 14 publications

(3 reference statements)

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“…Altho the latter group evolved undoubtedly from monocercomonads, we know of no organism which, like Hypotrichomonas, could be construed to represent a link between these primitive itrichomonads and Tritrichomonadinae. The phylogenetic kinship between a A4onoi-erromonas with a slender axostyle and Hypotrichomonas found support in nutritional and cultivation studies ( 53), which brought out also significant differences in the culture requirements of the 2 former organisms and Tritrichomonas. Additional and perhaps even more convincing evidence of the evolutionary position of Hypotrichomonas came from recent electronmicroscopic studies( 59).…”

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confidence: 89%

“…The correlation of light microscopic( see 35). electronmicroscopic (4,5,30,44,46,58,59,67 $4, and unpu'blished data) and nutritional (53) investigations also has contributed significantly to our understanding of the evolutionary trends among trichomonad flagellates. It was pstulated m e time ago on the basis of light microscopic observations(33,35, 52) that Hypotrichomonas, a trichomonad equipped with a feebly developed undulating membrane but lacking the costa, represented a link between the primitive monocercomonads (subfamily Rlonocercomonadinae) , which possess neither an undulating membrane nor a costa, and the members of the subfamily Trichomonadinae, as represented by Trirhomitus (= Tritrichomonas) batrachorum, all of which have a costa and most of which are characterized by a well-developed undulating memlbrane.…”

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confidence: 99%

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Some Recent Advances in Our Understanding of Parasitic Protozoa*†

Honigberg

1968

The Journal of Protozoology

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SYNOPSIS. Striking progress in our understanding of parasitic protoroa has been achieved thru the employment of advanced electronmicroscopic, biochemical, immunologic, and cultivation methods. Some recent information gathered by means of these methods on trichomonad and trypanosomalid flagellates as well a s on eimeriidian and plnsmotliid haemosporiidian Sporozoa is discussed. It is empha-T is evident from the recent reports published in a variety I of journals, review articles, and books that the most striking progress in our understanding of parasitic protozoa has been achieved thru the employment of advanced electronmicroscopic, biochemical, immunologic, and cultivation methods. Among the organisms about which much information has been accumulated by {these methods are the trypanosomatid and trichomonad flagellates and the plasmodiid haemosporidians. For this reason, and also because of my relatively greater familiarity with them, the following discussion will deal largely with these assemblages. The elimination of many other groups of parasitic protozoa from the present consideration must not be construed as reflecting lack of activity in their studies, but merely less activity and also poorer knowledge of them on my part.Several recent publications reviewing the parasitic protozoa (21,23,31,36,82,98) indicate (that data pertaining to biochemistry are based to a very significant degree upon studies of organisms grown in culture. Indeed, in many instances, analytic methods could not have been used without large yields of organisms achievable only through cultivation on nonliving media. The observations I propose to make concerning cultivation are relevant almost exclusively to extracellular species. Maintenance of intracellular forms in the presence of host cells or cell cultures, or even free of all such cells(for references see 36), has contributed much to our understanding of leishrnanias, and particularly of malarial parasites. These methods of cultivation involve somewhat different problems, which will not be considered here.

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confidence: 89%

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confidence: 99%

Some Recent Advances in Our Understanding of Parasitic Protozoa*†

Honigberg

1968

The Journal of Protozoology

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“…phospholipids, glycerides, Tweens and TEM's (tartaric acid esters of beef tallow monoglycerides ;Miller & Johnson, 1960;Soldo, Godoy & van Wagtendonk, 1966a). A s water dispersible sources of fatty acids, the TEM's are particularly effective in promoting growth and have gained increased application in the formulation of culture media for fastidious lipid-requiring micro-organisms (Shorb & Lund, 1959 ;Vogel & Hutner, 1961 ;Lee et al 1962). When one of these substances, TEM-4TY was used as a source of fatty acids for the cultivation of P. aurelia, it was observed that regulation of growth could be achieved by altering the relative concentrations of TEM-4T and stigmasterol in the medium.…”

Section: Introductionmentioning

confidence: 99%

Lipid Interrelationships in the Growth of Paramecium aurelia, Stock 299

Soldo¹,

Wagtendonk²

1968

Journal of General Microbiology

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In axenic medium with TEM-4T (a tartaric acid ester of beef tallow monoglyceride) as a source of fatty acids, regulation of the growth of Paramecium aurelia, stock 299, was achieved by varying the relative amounts of TEM-4T and stigmasterol. Optimal populations were reached in 7 days at 27' when the relative proportion of these lipids was 10 : I (w/w) TEM-4T : stigmasterol. Division was inhibited at ratios of 2: I (wiw) or less. The inhibition was annulled by restoration of the ' optimal ' ratios of TEM-4T : stigmasterol to the growth medium even after the protozoa had been exposed to 'inhibitory' ratios for periods up to 6 days. Growth was also inhibited when the relative proportions of TEM-4T : stigmasterol were adjusted to 2 : I (w/w) by the addition of TEM-4T to the culture after a 3-day period during which the organisms had been incubated with stigmasterol alone. No inhibition was observed when the TEM-4T: stigmasterol ratio was adjusted to 2 : I (wlw) by adding stigmasterol to the culture after a period of incubation with TEM-4T alone. It is suggested that under conditions of lipid imbalance in the medium stigmasterol interferes with the utilization of TEM-4T for growth.

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“…Lowering the trypticase concentration narrowed the temperature range for growth of T. augusta (Lee et al 1960). Finally, Lee et al_. (1962) reported that the addition of sjnall amounts of iron and copper to the medium extended the growth of T. augusta from 22°C.…”

Section: Concannonmentioning

confidence: 97%

“…Samuels (1958) reported the growth of T. augusta strain 101 over a pH range of 6.0-8.0. A later investigation on the effect of pH on T. augusta was done by Lee et. (1962) who reported growth in a pH range of 4.0-9.0, with an optimum pit range of 6.0-8.0.…”

Section: -87mentioning

confidence: 99%

The effects of environment on Tritrichomonas augusta (Alexeieff, 1911)

Soleymany-Kashi

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Trichomonads from Poikilotherms: Nutritional and Physiological Notes*

Cited by 10 publications

References 14 publications

Some Recent Advances in Our Understanding of Parasitic Protozoa*†

Some Recent Advances in Our Understanding of Parasitic Protozoa*†

Lipid Interrelationships in the Growth of Paramecium aurelia, Stock 299

The effects of environment on Tritrichomonas augusta (Alexeieff, 1911)

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