Trichoderma shennongjianum and Trichoderma tibetense, two new soil-inhabiting species in the Strictipile clade

Abstract

Morphology rpb2 Sequence analyses tef1 a b s t r a c t Two new species of Trichoderma are described based on soil samples from the Hubei and Tibet regions of China, and named as Trichoderma shennongjianum and Trichoderma tibetense.The combined analyses of morphology and sequence data, including culture characteristics, features of asexual states, and phylogeny inferred from partial sequences of translation elongation factor 1-a and the second largest RNA polymerase subunit encoding genes were carried out. Both species belong to the Strictipile clade, which previously contained only three species. Distinctions between the new species and their relatives are discussed. Trichoderma shennongjianum is related to T. longipile, and is characterized by variability in the sizes of its phialides and conidia and sequence data. Trichoderma tibetense appeared as a separate terminal branch related to T. strictipile, and is distinctive by its high growth rate and conidiophore branching pattern.

Discovery from a large-scaled survey of Trichoderma in soil of China

“…In summary of the recent studies including this work 11 , 12 , 18 , 32 , 38 , 41 – 45 , China is undoubtedly a hotspot of Trichoderma species diversity because more than 60 new species have been established and typified by the Chinese materials. However, our knowledge of the group in the country is far from sufficient compared with that in Europe 8 , 14 , 19 .…”

“…This result is in accordance with some earlier studies that southern China are of higher Trichoderma species diversity than the northern areas 16 , 17 . For example, seven new species were found from Hubei Province in addition to the previously described T. hubeiense and T. shennongjianum 18 , 38 . Hubei is relatively rich in Trichoderma species.…”

“…But it differs obviously from T. hamatum in the elongated conidiophores, which are straight and frequently fertile at the tips 14 . Phylogenetically, T. chlamydosporicum is closely related to T. tibetense , but the latter species differs in colony characteristics, slow growth on PDA, trichoderma-like conidiophores and smaller conidia (3.3–5.6 × 2.5–3.3) 18 .…”

“…They isolated 1910 strains from 20 provinces, and recognized 23 species based on morphological features and oligonucleotide barcode program (TrichOKEY v. 1.0 and TrichoBLAST). Most recently, three more species were added to the genus from Guizhou, Hubei and Tibet 11 , 18 .…”

Trichoderma Spp. as Antagonist of Rhizoctonia solani

“…Recently two new species of Trichoderma i.e. Trichoderma shennongjianum and Trichoderma tibetense have been isolated from soil samples from the Hubei and Tibet regions of China [27] and hopefully, these two-new species will be applied to manage R. solani in Future. …”