Trichoderma-Mediated ZnO Nanoparticles and Their Antibiofilm and Antibacterial Activities

Abstract

Antimicrobial resistance is a major global health concern and one of the gravest challenges to humanity today. Antibiotic resistance has been acquired by certain bacterial strains. As a result, new antibacterial drugs are urgently required to combat resistant microorganisms. Species of Trichoderma are known to produce a wide range of enzymes and secondary metabolites that can be exploited for the synthesis of nanoparticles. In the present study, Trichoderma asperellum was isolated from rhizosphere soil and used for the biosynthesis of ZnO NPs. To examine the antibacterial activity of ZnO NPs against human pathogens, Escherichia coli and Staphylococcus aureus were used. The obtained antibacterial results show that the biosynthesized ZnO NPs were efficient antibacterial agents against the pathogens E. coli and S. aureus, with an inhibition zone of 3–9 mm. The ZnO NPs were also effective in the prevention of S. aureus biofilm formation and adherence. The current work shows that the MIC dosages of ZnO NPs (25, 50, and 75 μg/mL) have effective antibacterial activity and antibiofilm action against S. aureus. As a result, ZnO NPs can be used as a part of combination therapy for drug-resistant S. aureus infections, where biofilm development is critical for disease progression.

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Green route to fabrication of Semal-ZnO nanoparticles for efficient solar-driven catalysis of noxious dyes in diverse aquatic environments

“…Fringes were observed in the TEM selected area electron diffraction (SAED) pattern at distances of 3.0125, 2.7129, and 2.4157, respectively, corresponding to the (100), (002), and (101) hkl planes ( Baruah et al, 2021 ) ( Figures 3C,D ), indicating the crystalline nature of the NPs. This is strongly corroborated by the XRD results, which demonstrates that the particles were crystalline ( Rambabu et al, 2021 ; Shobha et al, 2023 ).…”

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