Trichoderma harzianum metabolites disturb Fusarium culmorum metabolism: Metabolomic and proteomic studies

Mironenka, Julia; Różalska, Sylwia; Soboń, Adrian; Bernat, Przemysław

doi:10.1016/j.micres.2021.126770

Cited by 18 publications

(21 citation statements)

References 58 publications

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“…The behavior of the systems after the addition of the herbicides and Fusarium , in which Trichoderma extract was used, was comparable with the control system. This might have been due to a limitation in the development of Fusarium , as had been previously observed on PDA plates [ 3 ].…”

Section: Resultsmentioning

confidence: 91%

“…The emergence of such a persistent competitor as Trichoderma limits the pathogen development and results in the activation of defense mechanisms. The most effective method turned out to be the addition of T. harzianum extracts, which were found to influence the development of the fungus [ 3 ]. This observation is consistent with the study by Gromadzka et al [ 26 ], who showed that two various Trichoderma isolates reduced the amounts of ZEA produced by four different Fusarium strains in solid substrate bioassays.…”

Section: Resultsmentioning

confidence: 99%

“…Trichoderma harzianum KKP534 from the fungal strain collection of the Department of Industrial Microbiology and Biotechnology, University of Lodz, and the strain F. culmorum DSM 1094 purchased from the German Collection of Microorganisms and Cell Cultures GmbH were used in the study. The strains were selected for the study based on our previous research [ 3 ], where T. harzianum extracellular metabolites caused the inhibition of F. culmorum growth and development.…”

Section: Methodsmentioning

confidence: 99%

“…are soil-inhabiting filamentous fungi, including species with antagonistic activity against plant pathogens, such as Pythium spp., Fusarium spp., and others. The process of the pathogen growth inhibition depends on various mechanisms, such as production of lytic enzymes and antibiotics and much faster space growth and nutrient consumption [ 3 ]. Trichoderma species are well known for their ability to produce various metabolites with antibiotic activity including peptaibols, polyketides, polypeptides, pyrones, and terpenes.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous work, the impacts of 14-aminoacids peptaibols and the metabolites T22-azophilone and harzianic acid in T. harzianum extracellular extract on Fusarium culmorum growth and development together with zearalenone production were studied [ 3 ]. After their ability to reduce F. culmorum growth and ZEA production had been found, it was decided to continue research using both T. harzianum and its metabolites to prevent the development of the pathogen in the plant–fungal system with wheat.…”

Section: Introductionmentioning

confidence: 99%

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Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Mironenka

Różalska

Bernat

2021

IJMS

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Wheat is a critically important crop. The application of fungi, such as Trichoderma harzianum, to protect and improve crop yields could become an alternative solution to synthetic chemicals. However, the interaction between the fungus and wheat in the presence of stress factors at the molecular level has not been fully elucidated. In the present work, we exposed germinating seeds of wheat (Triticum aestivum) to the plant pathogen Fusarium culmorum and the popular herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of T. harzianum or its extracellular metabolites. Then, the harvested roots and shoots were analyzed using spectrometry, 2D-PAGE, and MALDI-TOF/MS techniques. Although F. culmorum and 2,4-D were found to disturb seed germination and the chlorophyll content, T. harzianum partly alleviated these negative effects and reduced the synthesis of zearalenone by F. culmorum. Moreover, T. harzianum decreased the activity of oxidoreduction enzymes (CAT and SOD) and the contents of the oxylipins 9-Hode, 13-Hode, and 13-Hotre induced by stress factors. Under the influence of various growth conditions, changes were observed in over 40 proteins from the wheat roots. Higher volumes of proteins and enzymes performing oxidoreductive functions, such as catalase, ascorbate peroxidase, cytochrome C peroxidase, and Cu/Zn superoxide dismutase, were found in the Fusarium-inoculated and 2,4-D-treated wheat roots. Additionally, observation of the level of 12-oxo-phytodienoic acid reductase involved in the oxylipin signaling pathway in wheat showed an increase. Trichoderma and its metabolites present in the system leveled out the mentioned proteins to the control volumes. Among the 30 proteins examined in the shoots, the expression of the proteins involved in photosynthesis and oxidative stress response was found to be induced in the presence of the herbicide and the pathogen. In summary, these proteomic and metabolomic studies confirmed that the presence of T. harzianum results in the alleviation of oxidative stress in wheat induced by 2,4-D or F. culmorum.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Mironenka

Różalska

Bernat

2021

IJMS

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Effects of Trichoderma harzianum biofertilizer on growth, yield, and quality of Bupleurum chinense

Liu

Cao³

et al. 2022

Plant Direct

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The use of chemical fertilizers and pesticides led to a decline in the quality and yield of Bupleurum chinense. The aim of this study was to determine the effects of Trichoderma harzianum biofertilizer on the growth, yield, and quality of radix bupleuri and microbial responses. The results showed that T. harzianum biofertilizer promoted the growth of B. chinense and increased the yield and quality of radix bupleuri. In addition, it increased the contents of NH4+–N, NO3−–N, available K, and available P and increased the activities of sucrase and catalase in the rhizosphere soil. High‐throughput analysis showed that the dominant bacteria in the rhizosphere were Proteobacteria (28%), Acidobacteria (23%), and Actinobacteria (17%), whereas the dominant fungi were Ascomycota (49%), Zygomycota (30%), and Basidiomycota (6%). After the application of T. harzianum biofertilizer, the abundance of Proteobacteria and Actinobacteria (relative to total bacteria) and Ascomycota and Basidiomycota (relative to total fungi) increased, but the relative abundance of Acidobacteria decreased. Canonical correlation analysis (CCA) showed that the relative abundance of Pseudarthrobacter, Streptomyces, Rhizobium, Nocardioides, Minimedusa, and Chaetomium were positively correlated with NO3−–N, NH4+–N, available K, available P, sucrase, and catalase in microbial communities, whereas Aeromicrobium and Mortierella were positively correlated with soil organic matter and urease. These results suggest that T. harzianum biofertilizer could significantly improve the yield and quality of radix bupleuri by changing the structure of soil microbial flora and soil enzyme activity. Therefore, it could be recommended for commercial scale production of Bupleurum.

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A novel method for the extraction and characterization of metabolites from Basidiomycota: Pleurotus ostreatus (Jacq.) P. Kumm., 1871 as a case study

Benvenuti,

Piazza,

Salis

et al. 2023

Separation Science Plus

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In recent years, the interest in the exploitation of fungal metabolites has grown considerably, given their application in numerous sectors involving human health. However, their identification and characterization by conventional analytical approaches is generally limited to single families of molecules per method of analysis. This constitutes a limiting factor of primary importance in the study of both the metabolic pattern of a single fungal sample and the discovery of its possible applications. In this work, a reverse‐phase high‐performance liquid chromatography coupled with mass spectrometry method for the profile determination of primary and secondary metabolites produced by the oyster‐mushroom Pleurotus ostreatus (Jacq.) P. Kumm., 1871, has been developed. By using a concomitant extraction in three different polarity‐decreasing solvents, namely methanol, ethanol, and acetonitrile, this method allowed the simultaneous analysis of all extracted metabolites belonging to the widest possible range of chemical families, giving an advantage for both qualitative and quantitative determination of known and unknown compounds. The method appears to be valuable and robust for the study of complex matrices like raw fungi extract such as those of Pleurotus ostreatus cultivated on different substrates and/or exposed to multiple stressors.

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Trichoderma harzianum metabolites disturb Fusarium culmorum metabolism: Metabolomic and proteomic studies

Cited by 18 publications

References 58 publications

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Effects of Trichoderma harzianum biofertilizer on growth, yield, and quality of Bupleurum chinense

A novel method for the extraction and characterization of metabolites from Basidiomycota: Pleurotus ostreatus (Jacq.) P. Kumm., 1871 as a case study

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