Trichoderma as a powerful fungal disease control agent for a more sustainable and healthy agriculture: recent studies and molecular insights

Rodrigues, Amanda Oliveira; Mio, Louise Larissa May De; Soccol, Carlos Ricardo

doi:10.1007/s00425-022-04053-4

Cited by 26 publications

(10 citation statements)

References 105 publications

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“…For all these reasons, in this work, biological control based on the application of Trichoderma species was evaluated as an alternative strategy for the control of G. castaneae in chestnut groves. The implementation of biological control against plant pathogens in agro-ecosystems by releasing competent Trichoderma strains is a safe technology that could solve issues related to environmental and health risks inherent with conventional control strategies [ 40 , 41 ]. Fungi of the genus Trichoderma are innate with the environment, being common inhabitants of soils, both agricultural and forest, and are often also found as endophytes in many plant species [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Trunk Injection Delivery of Biocontrol Strains of Trichoderma spp. Effectively Suppresses Nut Rot by Gnomoniopsis castaneae in Chestnut (Castanea sativa Mill.)

Benigno,

Aglietti,

Cacciola

et al. 2024

Biology

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Gnomoniopsis castaneae is responsible for brown or chalky nut rot in sweet chestnut (Castanea sativa), causing heavy reductions in nut production. Controlling it is challenging, due to its inconspicuous infections, erratic colonization of host tissues and endophytic lifestyle. Fungicides are not applicable because they are prohibited in chestnut groves. Trichoderma species are safe and wide-spectrum biocontrol agents (BCAs), with a variety of beneficial effects in plant protection. This study tested selected strains of T. viride, T. harzianum and T. atroviride for their ability to suppress G. castaneae. Field experiments were conducted in four chestnut groves (two test plots plus two controls) at two sites with a different microclimate. As the size of the trees were a major drawback for uniform and effective treatments, the Trichoderma strains were delivered directly by trunk injection, using the BITE® (Blade for Infusion in TrEes) endotherapic tool. The BCA application, repeated twice in two subsequent years, significantly reduced nut rot incidence, with a more marked, presumably cumulative, effect in the second year. Our data showed the tested Trichoderma strains retain great potential for the biological control of G. castaneae in chestnut groves. The exploitation of Trichoderma spp. as biopesticides is a novelty in the forestry sector and proves the benefits of these microbes in plant disease protection.

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Section: Discussionmentioning

confidence: 99%

Trunk Injection Delivery of Biocontrol Strains of Trichoderma spp. Effectively Suppresses Nut Rot by Gnomoniopsis castaneae in Chestnut (Castanea sativa Mill.)

Benigno,

Aglietti,

Cacciola

et al. 2024

Biology

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“…are being incorporated into crop plants by genetic engineering. In developing diseases resistant transgenic plants, a crucial step in the recognition of an efficient source and then introduction into the target crops (Rodrigues et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of Trichoderma Glucanase Gene for Plant Transformation

2023

IJAB

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Trichoderma harzianum is a potential biocontrol agent. A number of mechanisms showing their mycoparasitic ability have been explored i.e., antibiotic production, production of hydrolytic enzymes, parasitism, competition for nutrients, defense mechanism stimulations, and combination of all these. Considering the importance of Trichoderma harzianum derived glucanases in mycoparasitism, the present research work was planned to identify, isolate and characterize pathogenesis-related β-1, 6-glucanases gene from local strain of mycoparasite for plant transformation. Trichoderma harzianum genomic DNA was isolated by using modified CTAB method. Particular restriction sites were engineered at the far ends of β-1, 6-glucanase gene by Polymerase Chain Reaction. The amplified glucanase gene with engineered sites was cloned into pTZ57R/T cloning vector. Cloned fragment was sequenced and the resultant sequence was analyzed by using various bioinformatics tools i.e., Blast, Restriction mapper. It was found to have more than 97% homology with glucanase sequences reported in literature. Then regulatory sequences were cloned at the far ends of characterized glucanase gene for the development of plant transformation vector.

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“…Attempts to generate disease-suppressive soils in agriculture are a long-standing example of manipulating local species pools to limit pathogen availability for plant colonization [ 12 ]. In modern biocontrol, inoculations with the generalist fungus Trichoderma are common and often successful, likely due to its diverse lifestyles [ 13 ]. Environmental modifications might take the form of planned mosaics of agricultural and natural vegetation to generate more diverse source pools of fungi, as seen for foliar fungi in crops with more abundant natural vegetation located within 1 km of the farm [ 14 ].…”

Section: Manipulating Community Assembly Processesmentioning

confidence: 99%

Manipulating the plant mycobiome to enhance resilience: Ecological and evolutionary opportunities and challenges

Hawkes,

Allen,

Balint-Kurti

et al. 2023

PLoS Pathog

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Trichoderma as a powerful fungal disease control agent for a more sustainable and healthy agriculture: recent studies and molecular insights

Cited by 26 publications

References 105 publications

Trunk Injection Delivery of Biocontrol Strains of Trichoderma spp. Effectively Suppresses Nut Rot by Gnomoniopsis castaneae in Chestnut (Castanea sativa Mill.)

Trunk Injection Delivery of Biocontrol Strains of Trichoderma spp. Effectively Suppresses Nut Rot by Gnomoniopsis castaneae in Chestnut (Castanea sativa Mill.)

Cloning and Characterization of Trichoderma Glucanase Gene for Plant Transformation

Manipulating the plant mycobiome to enhance resilience: Ecological and evolutionary opportunities and challenges

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