Tricho- and atrichoblast cell files show distinct PIN2 auxin efflux carrier exploitations and are jointly required for defined auxin-dependent root organ growth

Löfke, Christian; Scheuring, David; Dünser, Kai; Schöller, Maria; Luschnig, Christian; Kleine‐Vehn, Jürgen

doi:10.1093/jxb/erv282

Cited by 20 publications

(21 citation statements)

References 38 publications

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“…As reported (Löfke et al, 2015), the average number of PIN2-GFPlabeled BFA bodies in trichoblast cells (1.73 6 0.65) was slightly higher than that of atrichoblast cells (1.46 6 0.46) in the wild type (Supplemental Fig. S13B).…”

Section: Sa But Not 24-d Affects the Membrane Association Of The Asupporting

confidence: 73%

“…Recent work showing that PIN2 endocytosis is differentially regulated between trichoblast versus atrichoblast cells in root epidermis (Löfke et al, 2015) prompted us to examine whether the loss of AP2s or AP2m and CME effectors/inhibitor differentially impacts PIN2 internalization in these two different cell types. As reported (Löfke et al, 2015), the average number of PIN2-GFPlabeled BFA bodies in trichoblast cells (1.73 6 0.65) was slightly higher than that of atrichoblast cells (1.46 6 0.46) in the wild type (Supplemental Fig.…”

Section: Sa But Not 24-d Affects the Membrane Association Of The Amentioning

confidence: 99%

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Differential Regulation of Clathrin and Its Adaptor Proteins during Membrane Recruitment for Endocytosis

Wang

et al. 2016

Plant Physiol.

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In plants, clathrin-mediated endocytosis (CME) is dependent on the function of clathrin and its accessory heterooligomeric adaptor protein complexes, ADAPTOR PROTEIN2 (AP-2) and the TPLATE complex (TPC), and is negatively regulated by the hormones auxin and salicylic acid (SA). The details for how clathrin and its adaptor complexes are recruited to the plasma membrane (PM) to regulate CME, however, are poorly understood. We found that SA and the pharmacological CME inhibitor tyrphostin A23 reduce the membrane association of clathrin and AP-2, but not that of the TPC, whereas auxin solely affected clathrin membrane association, in Arabidopsis (Arabidopsis thaliana). Genetic and pharmacological experiments revealed that loss of AP2m or AP2s partially affected the membrane association of other AP-2 subunits and that the AP-2 subunit AP2s, but not AP2m, was required for SA-and tyrphostin A23-dependent inhibition of CME. Furthermore, we show that although AP-2 and the TPC are both required for the PM recruitment of clathrin in wild-type cells, the TPC is necessary for clathrin PM association in AP-2-deficient cells. These results indicate that developmental signals may differentially modulate the membrane recruitment of clathrin and its core accessory complexes to regulate the process of CME in plant cells.

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Section: Sa But Not 24-d Affects the Membrane Association Of The Asupporting

confidence: 73%

Section: Sa But Not 24-d Affects the Membrane Association Of The Amentioning

confidence: 99%

Differential Regulation of Clathrin and Its Adaptor Proteins during Membrane Recruitment for Endocytosis

Wang

et al. 2016

Plant Physiol.

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“…The localization and endosomal recycling rates of PIN2 depend on auxin and other hormones (Paciorek et al, 2005;Willige et al, 2011;Löfke et al, 2013;Marhavý et al, 2014), light (Laxmi et al, 2008;Wan et al, 2012), temperature (Shibasaki et al, 2009;Hanzawa et al, 2013), cell types (Löfke et al, 2015), and gravity (Abas et al, 2006;Rahman et al, 2010).…”

Section: Escrt Gravitropism and Pinsmentioning

confidence: 99%

Role of SKD1 Regulators LIP5 and IST1-LIKE1 in Endosomal Sorting and Plant Development

et al. 2016

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SKD1 is a core component of the mechanism that degrades plasma membrane proteins via the Endosomal Sorting Complex Required for Transport (ESCRT) pathway. Its ATPase activity and endosomal recruitment are regulated by the ESCRT components LIP5 and IST1. How LIP5 and IST1 affect ESCRT-mediated endosomal trafficking and development in plants is not known. Here we use Arabidopsis mutants to demonstrate that LIP5 controls the constitutive degradation of plasma membrane proteins and the formation of endosomal intraluminal vesicles. Although lip5 mutants were able to polarize the auxin efflux facilitators PIN2 and PIN3, both proteins were mis-sorted to the tonoplast in lip5 root cells. In addition, lip5 root cells over-accumulated PIN2 at the plasma membrane. Consistently with the trafficking defects of PIN proteins, the lip5 roots showed abnormal gravitropism with an enhanced response within the first 4 h after gravistimulation. LIP5 physically interacts with IST1-LIKE1 (ISTL1), a protein predicted to be the Arabidopsis homolog of yeast IST1. However, we found that Arabidopsis contains 12 genes coding for predicted IST1-domain containing proteins (ISTL1-12). Within the ISTL1-6 group, ISTL1 showed the strongest interaction with LIP5, SKD1, and the ESCRT-III-related proteins CHMP1A in yeast two hybrid assays. Through the analysis of single and double mutants, we found that the synthetic interaction of LIP5 with ISTL1, but not with ISTL2, 3, or 6, is essential for normal plant growth, repression of spontaneous cell death, and post-embryonic lethality.

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“…Consistent with this, the process of plant endocytosis seems to be cell-type dependent (Baral et al, 2015;Löfke et al, 2015) and is responsive to endogenous plant signaling molecules (e.g. auxin, salicylic acid; Yu et al, 2016) as well as other environmental stimuli including plant pathogens (Smith et al, 2014b;Mbengue et al, 2016;Li and Pan, 2017), nutrients (Takano et al, 2010;Barberon et al, 2014), and light Zhang et al, 2017).…”

mentioning

confidence: 61%

Inroads into Internalization: Five Years of Endocytic Exploration

et al. 2017

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Tricho- and atrichoblast cell files show distinct PIN2 auxin efflux carrier exploitations and are jointly required for defined auxin-dependent root organ growth

Abstract: HighlightPIN2 shows distinct trafficking and vacuolar turnover in neighbouring tricho- and atrichoblast cell files. Differential abundance of PIN2 in the root epidermis could have developmental importance for root gravitropism.

Cited by 20 publications

References 38 publications

Differential Regulation of Clathrin and Its Adaptor Proteins during Membrane Recruitment for Endocytosis

Differential Regulation of Clathrin and Its Adaptor Proteins during Membrane Recruitment for Endocytosis

Role of SKD1 Regulators LIP5 and IST1-LIKE1 in Endosomal Sorting and Plant Development

Inroads into Internalization: Five Years of Endocytic Exploration

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