Trichloroethylene degradation by two independent aromatic-degrading pathways in Alcaligenes eutrophus JMP134

Harker, Alan R.; Kim, Y

doi:10.1128/aem.56.4.1179-1181.1990

Cited by 99 publications

(27 citation statements)

References 19 publications

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“…A mutant strain of JMP134 grows with 2,4-dichlorophenol and with 4-chloro-2-methylphenol [ 121. Trichloroethylene degradation mediated by aromatic catabolic pathways in strain JMP134 has also been reported [13]. In the present work, we further explore the degradative abilities of A. eutrophus JMP134, by growing it in cultures containing chlorophenols as a sole carbon and energy source.…”

Section: Introductionmentioning

confidence: 75%

Degradation of trichlorophenols by Alcaligenes eutrophus JMP134

CLEMENT

1995

FEMS Microbiology Letters

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The degradation of chlorophenols by Alcaligenes eutrophus JMP134 (pJP4) was studied. The strain grew on 2,4,6-trichlorophenol or 2,4,6-tribromophenol as the sole carbon and energy source. Complete degradation of 2,4,6-trichlorophenol was confirmed by chloride release and gas chromatography analysis of supernatants from growth cultures. The 2,3,5-, 2,3,4-, 2,3,6- and 2,4,5-isomers of trichlorophenol did not support growth. However, up to 40% of 2,4,5-trichlorophenol was mineralized during growth of A. eutrophus on chemostats fed with either phenol (0.4 mM) or 2,4,6-trichlorophenol (0.4 mM) plus 2,4,5-trichlorophenol (0.1 mM). Growth on 2,4,6-trihalophenols was also observed in A. eutrophus JMP222, the strain lacking pJP4, suggesting that this new degradative ability reported for A. eutrophus is not related to pJP4 encoded catabolic functions.

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Section: Introductionmentioning

confidence: 75%

Degradation of trichlorophenols by Alcaligenes eutrophus JMP134

CLEMENT

1995

FEMS Microbiology Letters

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“…Under methanogenic conditions, degradation was found to be relatively slow and mostly resulted in the accumulation of vinyl chloride, which is even more toxic (2,4,10,35,36). Laboratory studies have shown that TCE can be transformed aerobically by several oxygenase-producing bacterial cultures, including toluene-oxidizing strains (12,23,37,41), the ammonia-oxidizer Nitrosomonas europaea (1,34), and a propane-oxidizing Mycobacterium vaccae (38).…”

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confidence: 99%

Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichosporium OB3b and toxicity of trichloroethylene

Oldenhuis

Oedzes

Waarde

et al. 1991

Appl Environ Microbiol

287

187

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The kinetics of the degradation of trichloroethylene (TCE) and seven other chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b were studied. All experiments were performed with cells grown under copper stress and thus expressing soluble methane monooxygenase. Compounds that were readily degraded included chloroform, trans-1,2-dichloroethylene, and TCE, with Vmax values of 550, 330, and 290 nmol min'mg of cells-1, respectively. 1,1-Dichloroethylene was a very poor substrate. TCE was found to be toxic for the cells, and this phenomenon was studied in detail. Addition of activated carbon decreased the acute toxicity of high levels of TCE by adsorption, and slow desorption enabled the cells to partially degrade TCE. TCE was also toxic by inactivating the cells during its conversion. The degree of inactivation was proportional to the amount of TCE degraded; maximum degradation occurred at a concentration of 2 ,umol of TCE mg of cells-'. During conversion of [14C]TCE, various proteins became radiolabeled, including the a-subunit of the hydroxylase component of soluble methane monooxygenase. This indicated that TCE-mediated inactivation of cells was caused by nonspecific covalent binding of degradation products to cellular proteins.

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“…In all cases examined so far, the active microorganisms contain catabolic oxygenases with relaxed substrate specificities which catalyze a fortuitous oxygenation of TCE upon being stimulated with their respective substrates (43). Such microorganisms include methanotrophs (1,9,13,14,16,21,30,37), propane oxidizers (41), ethylene oxidizers (13), toluene, phenol, or cresol oxidizers (10,12,26,27,28,42), 2,4dichlorophenoxyacetic acid oxidizers (12), and ammonia oxidizers (2,38).…”

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confidence: 99%

Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer

Henry

Grbić-Galić

1991

Appl Environ Microbiol

125

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Trichloroethylene (TCE)-transforming aquifer methanotrophs were evaluated for the influence of TCE oxidation toxicity and the effect of reductant availability on TCE transformation rates during methane starvation. TCE oxidation at relatively low (6 mg liter-') TCE concentrations significantly reduced subsequent methane utilization in mixed and pure cultures tested and reduced the number of viable cells in the pure culture Methylomonas sp. strain MM2 by an order of magnitude. Perchloroethylene, tested at the same concentration, had no effect on the cultures. Neither the TCE itself nor the aqueous intermediates were responsible for the toxic effect, and it is suggested that TCE oxidation toxicity may have resulted from reactive intermediates that attacked cellular macromolecules. During starvation, all methanotrophs tested exhibited a decline in TCE transformation rates, and this decline followed exponential decay. Formate, provided as an exogenous electron donor, increased TCE transformation rates in Methylomonas sp. strain MM2, but not in mixed culture MM1 or unidentified isolate, CSC-1. Mixed culture MM2 did not transform TCE after 15 h of starvation, but mixed cultures MM1 and MM3 did. The methanotrophs in mixed cultures MM1 and MM3, and the unidentified isolate CSC-1 that was isolated from mixed culture MM1 contained lipid inclusions, whereas the methanotrophs of mixed culture MM2 and Methylomonas sp. strain MM2 did not. It is proposed that lipid storage granules serve as an endogenous source of electrons for TCE oxidation during methane starvation.

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Trichloroethylene degradation by two independent aromatic-degrading pathways in Alcaligenes eutrophus JMP134

Cited by 99 publications

References 19 publications

Degradation of trichlorophenols by Alcaligenes eutrophus JMP134

Degradation of trichlorophenols by Alcaligenes eutrophus JMP134

Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichosporium OB3b and toxicity of trichloroethylene

Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer

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