Trichinella spiralis: Secreted antigen of the infective L1 larva localizes to the cytoplasm and nucleoplasm of infected host cells

Despommier, Dickson D.; Gold, Allen M.; Buck, Stephen W.; Capó, Virginia; Silberstein, David S.

doi:10.1016/0014-4894(90)90005-w

Cited by 77 publications

(41 citation statements)

References 27 publications

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“…38 Antigenic epitopes and monoclonal antibodies have been studied mainly for T. spiralis. 7,20 Some of the research has focused on tyvelose-bearing antigens involved in penetration of the gut epithelium. 22 Characterization of T. spiralis antigens has shown that some are stage specific.…”

Section: Discussionmentioning

confidence: 99%

Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

et al. 2002

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Abstract. Three experimental groups of six male raccoon dogs (Nyctereutes procyonoides) each were formed by placing one of three littermates from six litters into each group. One group was inoculated with pigorigin Trichinella spiralis, the second was inoculated with raccoon dog-origin T. nativa, and the third served as a control group. The infective dose was 1,000 larvae/kg of body weight. Every third week, biopsies from M. triceps brachii were taken, and serum samples were collected for up to 12 weeks postinfection. In the early phase of the infection, cysts of both parasites were elongated cylinders that later became more spherical. However, at the end of the experiment, the cysts of T. nativa were more rounded than those of T. spiralis (mean length/width ϭ 2.5 versus 1.5 in T. spiralis versus T. nativa, respectively). Both species accumulated a collagen-rich capsule around the nurse cell, but the capsule was thicker in T. nativa. In both parasites, the total surface area of the sagittal section of the cyst was equal. Inflammation was more intense around T. nativa cysts. Specific antibodies were recognizable 2 weeks after infection by both enzyme-linked immunosorbent assay (ELISA) and western blot. In western blots, serum from both T. nativa-and T. spiralis-infected animals recognized the same components, but reaction with the homologous antigen was stronger. The same pattern was also seen in the ELISA. Immunoreactive epitopes were localized only in internal organs and cuticula of larvae in muscle.

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Section: Discussionmentioning

confidence: 99%

Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

et al. 2002

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“…This MAb is specific to a tyvelosecontaining glycan determinant synthesized by T. spiralis (1,8,21). Antideglycosylated p43 antibodies (␣-DG p43; IgG and IgA; kindly provided by Dickson Despommier) are rabbit antibodies raised against the p43 T. spiralis stichocyte protein as previously characterized (6,14). The nuclear binding characteristics of and the NA recognized by MAb 9D4 and ␣-DG p43 are indistinguishable (27).…”

Section: Methodsmentioning

confidence: 99%

“…These antigens are referred to as nuclear antigens (NA). NA become apparent in infected cell nuclei by 9 dpi and chronically persist in these nuclei for the duration of the infection (6). NA colocalize with host chromatin in the nucleoplasm, exclusive of nucleoli or the nucleoskeleton.…”

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confidence: 99%

Trichinella spiralis -Infected Muscle Cells: Abundant RNA Polymerase II in Nuclear Speckle Domains Colocalizes with Nuclear Antigens

Yao

Jasmer

2001

Infect Immun

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Infection of mammalian skeletal muscle cells by Trichinella spiralis causes host nuclei to become polyploid (ca. 4N) and abnormally enlarged. It has been postulated that this enlargement reflects an infection-induced elevation of host transcription. Anthelmintic treatment of T. spiralis-infected rodents with mebendazole (MBZ) causes a reduction in the size of infected cell nuclei and a significant reduction in the total RNA content of individual infected muscle cells. A monoclonal antibody to the large subunit of RNA polymerase II (Pol II) was used here to assess the effects of infection on Pol II levels in isolated infected cell nuclei. Pol II was localized to speckle domains in isolated infected cell nuclei. Similar domains have been previously localized to sites of RNA synthesis or processing. When compared to the levels in nuclei from other, uninfected host cells, speckle-localized Pol II (SL-Pol II) levels were significantly elevated in infected cell nuclei by a mean of 3.9-to 6.8-fold. Nuclear antigens (NA) recognized by antibodies against T. spiralis localized to infected cell nuclei. By use of confocal microscopy, a subpopulation of NA was found colocalized with most speckle domains defined by Pol II. MBZ treatment of chronically infected mice, which depletes NA from infected cell nuclei, caused a significant depletion of SL-Pol II from infected cell nuclei. Control nuclei had a mean of 70% more SL-Pol II than MBZ-treated nuclei. The mean residual level of Pol II in these polyploid nuclei remained elevated by 120% over the level in 2N control nuclei. These observations may indicate two distinct effects of infection on Pol II levels in host cells.

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“…Trichinella infection causes local and systemic changes in the host. The former includes muscle cell transformation to a nurse cell in the cyst [6], and the latter includes immunological responses which act in two ways: one is resisting the second challenge of Trichinella infection and the other is suppressing the immune attack against the already-existing Trichinella in the host muscle. In relation to this research interest, we have previously studied Fig.…”

Section: Animals and Infectionmentioning

confidence: 99%

Effect of nematode <i>Trichinella</i> infection on glucose tolerance and status of macrophage in obese mice

et al. 2013

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Trichinella spiralis: Secreted antigen of the infective L1 larva localizes to the cytoplasm and nucleoplasm of infected host cells

Cited by 77 publications

References 27 publications

Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

Trichinella nativaandT. spiralisInduce Distinguishable Histopathologic and Humoral Responses in the Raccoon Dog (Nyctereutes procyonoides)

Trichinella spiralis -Infected Muscle Cells: Abundant RNA Polymerase II in Nuclear Speckle Domains Colocalizes with Nuclear Antigens

Effect of nematode <i>Trichinella</i> infection on glucose tolerance and status of macrophage in obese mice

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