Tributyltin exposure disrupted the locomotor activity rhythms in adult zebrafish (Danio rerio) and the mechanism involved

Guo, Rui-ying; Xiang, Jing; Wang, Lijun; Li, Erchao; Zhang, Jiliang

doi:10.1016/j.aquatox.2022.106287

Cited by 8 publications

(4 citation statements)

References 51 publications

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“…Exposure to 0.37 µg STXeq/mL induced paralysis by 46 h on 2 days post-fertilization (dpf) embryos, and the same effect was observed by exposure times of eight and five hours in 4 dpf and 6 dpf larvae, respectively, with higher mortality rates in the latter cases [5]. The locomotor activity in adults and larval zebrafish is a simple behavior that has been reported to be affected by several drugs and environmental pollutants at sub-lethal concentrations [21][22][23][24][25][26] and is well known to be influenced by age, exposure volume, photoperiod, and group size [27][28][29].…”

Section: Introductionmentioning

confidence: 87%

A New Bioassay for the Detection of Paralytic and Amnesic Biotoxins Based on Motor Behavior Impairments of Zebrafish Larvae

Paz

Zambrano

Ortiz

et al. 2023

IJMS

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The global concern about the increase of harmful algal bloom events and the possible impacts on food safety and aquatic ecosystems presents the necessity for the development of more accessible techniques for biotoxin detection for screening purposes. Considering the numerous advantages that zebrafish present as a biological model and particularly as a toxicants sentinel, we designed a sensitive and accessible test to determine the activity of paralytic and amnesic biotoxins using zebrafish larvae immersion. The ZebraBioTox bioassay is based on the automated recording of larval locomotor activity using an IR microbeam locomotion detector, and manual assessment of four complementary responses under a simple stereoscope: survival, periocular edema, body balance, and touch response. This 24 h acute static bioassay was set up in 96-well microplates using 5 dpf zebrafish larvae. For paralytic toxins, a significant decrease in locomotor activity and touch response of the larvae was detected, allowing a detection threshold of 0.1–0.2 µg/mL STXeq. In the case of the amnesic toxin the effect was reversed, detecting hyperactivity with a detection threshold of 10 µg/mL domoic acid. We propose that this assay might be used as a complementary tool for environmental safety monitoring.

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Section: Introductionmentioning

confidence: 87%

A New Bioassay for the Detection of Paralytic and Amnesic Biotoxins Based on Motor Behavior Impairments of Zebrafish Larvae

Paz

Zambrano

Ortiz

et al. 2023

IJMS

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“…Antifouling paints containing TBT are still manufactured and sold in certain countries (Uc-Peraza et al, 2022) [27] . Total organotin concentrations range values of 1.76-486.62 ng Sn/g (dry weight) in Asian harbors (Wang et al, 2019) [28] The visual system has a high sensitivity to TBT (Guo et al, 2022) [11] . TBT targets the retina and causes apoptosis in developing zebrafish which may be related to oxidative stress (Dong et al, 2006) [6] .…”

Section: Introductionmentioning

confidence: 99%

Effect of tributyltin exposure on the eye of zebrafish

Delvadiya,

Patel,

Makwana

et al. 2024

Int. J. Adv. Biochem. Res.

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Tributyltin (TBT), as an organotin substance, is recognized as one of the important xenobiotic pollutants. The present study was carried out to investigate the toxicological effects of TBT on the sensory organ eye of adult female zebrafish. Adult zebrafish were exposed to three concentrations of TBT for 28 days (125, 250, and 500 ng/L) to evaluate the oxidative stress markers and histological changes in the eye. Mortality was not observed in any toxicity group. TBT exposure at 250 and 500 ng/L significantly decreased the CAT activity and total antioxidant capacity (TAC) of eye tissue. The higher levels of TBT exposure (250 and 500 ng/L) significantly increased lipid peroxidation as indicated by increased levels of MDA. Histopathological evaluation of the retina of adult zebrafish following TBT exposure at the highest concentration revealed mild depigmentation of the retinal pigmented epithelium layer.

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“…During the early development stages of aquatic organisms, melanocytes secrete melanin to form patches to adapt the light changes of the environment as required by survival needs such as self-protection. , Aquatic organisms adjust the distribution of melanin in skin according to the changes in light levels because melanocytes are photosensitive and regulated by circadian rhythm, which depends largely on the retinal receipt of light. − Visually impaired aquatic organisms are unable to detect light changes and thus lose the ability of adjusting skin color to adapt to the surrounding environment. Many environmental pollutants, such as polybrominated diphenyl ethers (TPT), and triclocarban (TCC) may affect eye development of aquatic organisms and alter the phototransduction cascade and circadian network. , This makes them insensitive to light changes and ultimately result in abnormal pigmentation and reducing their overall survival rates. − Therefore, pigmentation is considered to be an important and visually observable toxic endpoint of developmental toxicity caused by environmental pollutants . It was reported that some PFAS could cross the blood-retina barrier and accumulate in the eye of zebrafish, thus impairing the visual development and function. , However, previous studies did not pay attention to the relationship between visual impairment and skin pigmentation.…”

Section: Introductionmentioning

confidence: 99%

“…Many environmental pollutants, such as polybrominated diphenyl ethers (TPT), and triclocarban (TCC) may affect eye development of aquatic organisms and alter the phototransduction cascade and circadian network. 22,23 This makes them insensitive to light changes and ultimately result in abnormal pigmentation and reducing their overall survival rates. 24−27 Therefore, pigmentation is considered to be an important and visually observable toxic endpoint of developmental toxicity caused by environmental pollutants.…”

Section: Introductionmentioning

confidence: 99%

Novel Insights on 6:6 Perfluoroalkyl Phosphonic Acid-Induced Melanin Synthesis Disorders Leading to Pigmentation in Tadpoles

Zhu

Sun

Yang

et al. 2023

Environ. Sci. Technol.

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As alternatives to traditional per- and polyfluoroalkyl substances, perfluoroalkyl phosphonic acids (PFPiAs) are widely present in aquatic environments and can potentially harm aquatic organisms. Pigmentation affects the probability of aquatic organisms being preyed on and serves as an important toxic endpoint of development, but little is known about the impacts of PFPiAs on the development of aquatic organisms. In this study, Xenopus laevis embryos were exposed to 6:6 PFPiA (1, 10, and 100 nM) for 14 days. The developed tadpoles exhibited evident pigmentation with increased melanin particle size and density on the skin. Pathological and behavioral experiments revealed that the retinal layers became thinner, reducing the photosensitivity and disturbing the circadian rhythm of the tadpoles. Compared to the control group, the exposed tadpoles showed higher levels but less changes of melanin throughout the light/dark cycle, as well as distinct oxidative damage. Consequently, the expression level of microphthalmia-associated transcription factor (MITF), a key factor inducing melanin synthesis, increased significantly. Molecular docking analysis suggested that 6:6 PFPiA forms strong interactions in the binding pocket of MITF, implying that it could activate MITF directly. The activation of MITF ultimately promotes melanin synthesis, resulting in pigmentation on tadpoles.

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Tributyltin exposure disrupted the locomotor activity rhythms in adult zebrafish (Danio rerio) and the mechanism involved

Cited by 8 publications

References 51 publications

A New Bioassay for the Detection of Paralytic and Amnesic Biotoxins Based on Motor Behavior Impairments of Zebrafish Larvae

A New Bioassay for the Detection of Paralytic and Amnesic Biotoxins Based on Motor Behavior Impairments of Zebrafish Larvae

Effect of tributyltin exposure on the eye of zebrafish

Novel Insights on 6:6 Perfluoroalkyl Phosphonic Acid-Induced Melanin Synthesis Disorders Leading to Pigmentation in Tadpoles

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