Tributyltin contamination of bivalves in coastal areas around northern Kyushu, Japan

Inoue, Suguru; Abe, Shingo; Oshima, Yuji; Kai, Norihisa; Honjo, Tasuku

doi:10.1002/tox.20177

Cited by 35 publications

(18 citation statements)

References 15 publications

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“…Their study revealed that though there was a decrease in the frequency of imposex occurrence in the coasts, no significant change in the frequency of imposex was observed in harbors and bays, indicating persistence of TBT. Widespread contamination of TBT is detectable along the coastal regions of many countries (Sudaryanto et al 2002;Inoue et al 2006;Kim and Kim 2008).…”

Section: Introductionmentioning

confidence: 99%

Biochemistry of TBT-Degrading Marine Pseudomonads Isolated from Indian Coastal Waters

Sampath

Venkatakrishnan

Ravichandran

et al. 2011

Water Air Soil Pollut

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Tributyltin (TBT) is a very effective biocide and an active ingredient in antifouling paints. Screening along the Indian coast yielded 49 bacterial isolates capable of TBT assimilation. The screening was done based on the ability of bacteria to grow in mineral salt medium (MSM) containing TBT as the sole source of carbon. All the isolates produced exopolysaccharides (biosurfactants) in the medium which aid in emulsification and thus ease bioavailability of TBT. Five isolates were identified as potent TBT degraders (namely, Pseudomonas pseudoalcaligenes, Pseudomonas stutzeri, Pseudomonas mendocina, Pseudomonas putida, and Pseudomonas balearica) based on their biomass production in MSM containing TBT as the sole source of carbon. In addition to evaluating the potential of individual bacterial strains, the study also focused on using a consortium of bacteria to explore their synergistic effect when grown on TBT. Further tests like growth profile, rhamnolipid secretion profile, extracellular protein secretion profile, and detection of siderophores were performed on these isolates when grown in MSM supplemented with 2 mM TBT concentration. Emulsification activity of the crude extracellular polysaccharides against kerosene was evaluated. It can be therefore inferred that TBT degradation by these marine pseudomonads is a twostep process: (a) dispersion of TBT in the aqueous phase and (b) tin-carbon bond cleavage by siderophores affecting debutylation of TBT. The consortium of bacteria may be effective in the treatment of TBT-contaminated waste water in dry docks.

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Section: Introductionmentioning

confidence: 99%

Biochemistry of TBT-Degrading Marine Pseudomonads Isolated from Indian Coastal Waters

Sampath

Venkatakrishnan

Ravichandran

et al. 2011

Water Air Soil Pollut

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“…The Ariake Sea is a typical semi-closed bay located in Kyushu Island, western Japan, with a maximum tidal range of 6 m in the spring tide. Inoue et al (2002) conducted on-the-spot examinations of TBT concentration in the northern Ariake Sea in 1998. In his examinations, the relatively high average TBT concentrations of 38.7 µg m −3 in the water and 4,382 µg m −3 (in unit volume of soil and water (i.e., wet weight); or 39.4 µg kg −1 in unit mass of dry soil (i.e., dry weight) at 2 cm below the bottom surface were observed at the head of the Ariake Sea, the estuary of Chikugo River, and offshore Miike Harbor.…”

Section: Study Areamentioning

confidence: 99%

“…As a result of this ban, the application of TBT in antifouling paints has become less important; however, TBT continues to adversely affect the aquatic environment, due to their use in large vessels and in countries without regulations (Fent 1996). On-the-spot examinations in semiclosed bays around Kyushu Island, Japan, revealed relatively high concentrations of TBT in coastal and harbor waters and bottom sediments (Inoue et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Numerical modeling of environmental behavior and fate of tributyltin in a semi-closed bay

Hiramatsu

Oshima

Inoue

et al. 2005

Paddy Water Environ

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The environmental behavior and fate of tributyltin (TBT) in the northern Ariake Sea, resulting from the use of TBT-containing antifouling paints on hulls of ships, pleasure crafts and docking facilities, was assessed by numerical simulations. First, a mathematical model was devised on the basis of a non-steady state equilibrium, one box multi-compartment model consisting of the surface micro-layer, the water column, the mud-layer, and the bottom sediment compartments. The movement of TBT among the four compartments was modeled by resuspension of bottom mud, deposition of suspended sediment, film penetration and water advection in each compartment. Furthermore, a one-dimensional diffusion equation was introduced into the bottom sediment compartment to calculate profile distribution of TBT. The reactivity of TBT considered within the compartments included biological degradation, adsorption to particulate matter and diffusion. Next, the optimal amount of past TBT loads, reflecting the recent observations of TBT concentration in the waters and sediments of the northern Ariake Sea, was searched by a simple genetic algorithm. The relative sensitivity of various model parameters were also determined to identify the more important parameters for estimating the environmental behavior and fate of TBT. Finally, the future status of TBT contamination of the northern Ariake Sea was predicted assuming the discontinued use of TBT-containing antifouling paints. Despite its simple model structure relative to hydraulics, it was concluded that this multi-compartment model adequately estimated the environmental behavior and fate of TBT.

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“…Among marine pollutants, organotins, such as TBT, are highly toxic to a wide range of organisms (Inoue et al 2006a(Inoue et al , 2006bOhji et al 2005;Antizar-Ladislao 2008). The use of TBT in antifouling paints has been prohibited since 2008 (IMO, International Maritime Organization, www.imo.org).…”

Section: Introductionmentioning

confidence: 99%

“…In aquatic animals, TBT is known to induce the development of male sex characteristics in females, particularly in several gastropods (Hagger et al 2006;Horiguchi 2006;Horiguchi et al 2006;Janer et al 2006;Viglino et al 2006), thus causing sterility. It also makes aquatic animals more vulnerable to infectious diseases (Anderson et al 1996) and has deleterious effects on oyster growth and reproduction (Alzieu 1991;Gagné et al 2003;Inoue et al 2004Inoue et al , 2006aInoue et al , 2006bInoue et al , 2007. Human exposure may arise from consumption of TBT-contaminated food products (Jurkeviewicz et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

Droguet

Devauchelle

Pennec

et al. 2012

Aquat. Living Resour.

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-European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10 −6 M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, significantly decreased after two days of treatment with 10 −6 M TBT, and after six days with 10 −10 M TBT. The percentage of apoptotic cells, quantified by flow cytometry and YO-PRO r -1 iodide, a nucleic acid stain that only permeates cells that are beginning to undergo apoptosis, increased significantly in these cases. Moreover, intracellular concentration of Ca ++ had increased after 10 min of exposition to 10 −6 M, and could be associated with apoptotic processes. As patch clamp experiments showed that Ca ++ conductance was decreased, intracellular calcium increase could mainly be due to a release from internal stores. The decreases in beating rhythm could be explained by the decrease in adenosine triphosphate (ATP) production revealed by 31 P nuclear magnetic resonance (NMR) spectroscopy and confirmed by the increase of the K ATP channel conductance. The related hyperpolarization and the disturbances of the energetic metabolism were clearly related to the loss of the atrial cell contractility and viability.

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Tributyltin contamination of bivalves in coastal areas around northern Kyushu, Japan

Cited by 35 publications

References 15 publications

Biochemistry of TBT-Degrading Marine Pseudomonads Isolated from Indian Coastal Waters

Biochemistry of TBT-Degrading Marine Pseudomonads Isolated from Indian Coastal Waters

Numerical modeling of environmental behavior and fate of tributyltin in a semi-closed bay

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

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