Tributyltin and triphenyltin exposure promotes in vitro adipogenic differentiation but alters the adipocyte phenotype in rainbow trout

Lutfi, Esmail; Riera‐Heredia, Natàlia; Córdoba, Marlon; Porte, Cinta; Gutiérrez, Joaquím; Capilla, Encarnación; Navarro, Isabel

doi:10.1016/j.aquatox.2017.05.001

Cited by 30 publications

(26 citation statements)

References 68 publications

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“…Key genes implicated in adipogenesis and energy metabolism regulation were analyzed by real-time quantitative PCR (qPCR) following the procedure previously described in a previous study in the group [72]. The genes evaluated comprise the following (Table 2): the transcription factors or nuclear receptors: cebpa, cebpb, ppara, pparb, pparg, lxr, and rxr; the enzymes: fas, lpl, and hsl; and lipid transporters: cd36, fatp1, fabp11, and ATP-binding cassette transporter (abca1).…”

Section: Quantitative Pcr Analysesmentioning

confidence: 99%

Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout (Oncorhynchus mykiss)

Riera‐Heredia

Lutfi

Sánchez-Moya

et al. 2020

IJMS

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Fish are rich in n-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Due to the increasing use of vegetable oils (VO), their proportion in diets has lowered, affecting lipid metabolism and fillet composition. Rainbow trout cultured preadipocytes were treated with representative FA found in fish oils (EPA and DHA) or VO (linoleic, LA and alpha-linolenic, ALA acids), while EPA and LA were also orally administered, to evaluate their effects on adipogenesis and lipid metabolism. In vitro, all FA increased lipid internalization, with ALA producing the highest effect, together with upregulating the FA transporter fatp1. In vivo, EPA or LA increased peroxisome proliferator-activated receptors ppara and pparb transcripts abundance in adipose tissue, suggesting elevated β-oxidation, contrary to the results obtained in liver. Furthermore, the increased expression of FA synthase (fas) and the FA translocase/cluster of differentiation (cd36) in adipose tissue indicated an enhanced uptake of lipids and lipogenesis de novo, whereas stable or low hepatic expression of genes involved in lipid transport and turnover was found. Thus, fish showed a similar tissue metabolic response to the short-term availability of EPA or LA in vivo, while in vitro VO-derived FA demonstrated greater potential inducing fat accumulation.

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Section: Quantitative Pcr Analysesmentioning

confidence: 99%

Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout (Oncorhynchus mykiss)

Riera‐Heredia

Lutfi

Sánchez-Moya

et al. 2020

IJMS

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“…In the present study, primary culture of preadipocytes was used to investigate for the first time, to the best of our knowledge, the impacts of MeHg in rainbow trout preadipocytes. This in vitro model is a relevant tool to assess the effects of potential obesogenic compounds as suggested by Lutfi et al (2017). Specific culture conditions were applied to enable the differentiation of precursor cells.…”

Section: Model Of Primary Cultured Preadipocytes From Rainbow Troutmentioning

confidence: 99%

“…On the other hand, the liver transcriptome of HgCl 2 -treated zebrafish revealed the upregulation of fatty acid synthesis pathway within 96 h without, however, specifying the enzyme(s) involved (Ung et al, 2010). Triphenyltin (TPT), a well-known obesogen, also induced an increased expression of FASN in trout preadipocytes (Lutfi et al, 2017). On the other hand, after two and six days of contamination, a significant and negative impact of MeHg was observed on the expression of ACLY, another enzyme involved in de novo fatty acid synthesis pathway (Fig.…”

Section: Effects Of Mehg On Lipid Metabolism At Transcriptional Levelmentioning

confidence: 99%

“…This transcriptional cascade activates the expression of adipocyterelated genes and leads to the differentiated adipocyte phenotype. Various compounds such as polychlorobiphenyl (PCB) and polybrominated diphenyl ether congeners, bisphenol A or tributyltin (TBT) have been reported as obesogens in in vitro and in vivo animal and human models (Newbold et al, 2009;Ferrante et al, 2014;Ariemma et al, 2016;Lutfi et al, 2017). Obesogens mainly act through increasing the number or the size of adipocytes, or altering the endocrine regulation and the lipid homeostasis (Darbre, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Methylmercury displays pro-adipogenic properties in rainbow trout preadipocytes

et al. 2021

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h i g h l i g h t s MeHg induced a dose-dependent accumulation of neutral lipids in preadipocytes. Accumulation of lipids persisted beyond the cellular exposure to MeHg. MeHg increased n-3 PUFA levels in lipid droplets and dropped AA levels in membranes. Expression of adipocyte-and lipid-specific genes was modulated by MeHg. Total mercury accumulated in cells was released when MeHg exposure stopped.

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“…The ability of TBT and triphenyltin (TPT) to promote adipocytes differentiation was demonstrated in trout ( 60 ). Primary cultured adipocyte treated with TBT and TPT induced lipid accumulation and slightly enhanced PPARγ and C/EBPα protein expression, suggesting that the use of a primary adipocyte cell culture from this species is a valuable in vitro tool to estimate the capacity of different compounds and their synergism to interfere with adipocyte differentiation and lipid accumulation.…”

Section: Effects Of Edcs Exposure On Lipid Metabolism In Animal Modelmentioning

confidence: 99%

Lipid Metabolism Alteration by Endocrine Disruptors in Animal Models: An Overview

Maradonna

Carnevali

2018

Front. Endocrinol.

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Exposure to potential Endocrine Disrupting Chemicals (EDCs) pose a documented risk to both wildlife and human health. Many studies so far described declining sperm counts, genital malformations, early puberty onset, highlighting the negative impact on reproduction caused by the exposure to many anthropogenic chemicals. In the last years, increasing evidence suggested that these compounds, other than altering reproduction, affect metabolism and induce the onset of obesity and metabolic disorders. According to the “environmental obesogens” hypothesis, evidence exists that exposure to potential EDCs during critical periods when adipocytes are differentiating, and organs are developing, can induce diseases that manifest later in the life. This review summarizes the effects occurring at the hepatic level in different animal models, describing morphological alterations and changes of molecular pathways elicited by the toxicant exposure. Results currently available demonstrated that these chemicals impair normal metabolic processes via interaction with members of the nuclear receptor superfamily, including steroid hormone receptors, thyroid hormone receptors, retinoid X receptors, peroxisome proliferator–activated receptors, liver X receptors, and farnesoid X receptors. In addition, novel results revealed that EDC exposure can either affect circadian rhythms as well as up-regulate the expression of signals belonging to the endocannabinoid system, in both cases leading to a remarkable increase of lipid accumulation. These results warrant further research and increase the interest toward the identification of new mechanisms for EDC metabolic alterations. The last part of this review article condenses recent evidences on the ability of potential EDCs to cause “transgenerational effects” by a single prenatal or early life exposure. On this regard, there is compelling evidence that epigenetic modifications link developmental environmental insults to adult disease susceptibility. This review will contribute to summarize the mechanisms underlying the insurgence of EDC-induced metabolic alterations as well as to build integrated strategies for their better management. In fact, despite the large number of results obtained so far, there is still a great demand for the development of frameworks that can integrate mechanistic and toxicological/epidemiological observations. This would increase legal and governmental institution awareness on this critical environmental issue responsible for negative consequences in both wild species and human health.

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Tributyltin and triphenyltin exposure promotes in vitro adipogenic differentiation but alters the adipocyte phenotype in rainbow trout

Cited by 30 publications

References 68 publications

Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout (Oncorhynchus mykiss)

Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout (Oncorhynchus mykiss)

Methylmercury displays pro-adipogenic properties in rainbow trout preadipocytes

Lipid Metabolism Alteration by Endocrine Disruptors in Animal Models: An Overview

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