Tribromotoluquinone Induced Modifications of the Oscillation Pattern of Oxygen Evolution and of Herbicide Binding in Thylakoids and PS II Membrane Fragments from Spinach

Ренгер, Г.; Messinger, Johannes; Fromme, Raimund

doi:10.1515/znc-1989-5-614

Cited by 10 publications

(9 citation statements)

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“…This finding can be explained by the blockage of PS II due to photoinhibition and the assumption that the total pool capacity is available for the remaining active centers. An analogous effect has been observed recently in PS II-membrane fragments that were partially inhibited by DCMU (Renger et al 1989).…”

Section: Resultssupporting

confidence: 84%

Effects of photoinhibition on the PS II acceptor side including the endogenous high spin Fe2+ in thylakoids, PS II-membrane fragments and PS II core complexes

1992

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Effects of photoinhibition at 0 °C on the PS II acceptor side have been analyzed by comparative studies in isolated thylakoids, PS II membrane fragments and PS II core complexes from spinach under conditions where degradation of polypeptide(s) D1(D2) is highly retarded. The following results were obtained by measurements of the transient fluorescence quantum and oxygen yield, respectively, induced by a train of short flashes in dark-adapted samples: (a) in the control the decay of the fluorescence quantum yield is very rapid after the first flash, if the dark incubation was performed in the presence of 300 μM K3[Fe(CN)6]; whereas, a characteristic binary oscillation was observed in the presence of 100 μM phenyl-p-benzoquinone with a very fast relaxation after the even flashes (2nd, 4th. . . ) of the sequence; (b) illumination of the samples in the presence of K3[Fe(CN)6] for only 5 min with white light (180 W m(-2)) largely eliminates the very fast fluorescence decay after the first flash due to QA (-) reoxidation by preoxidized endogenous non-heme Fe(3+), while a smaller effect arises on the relaxation kinetics of the fluorescence transients induced by the subsequent flashes; (c) the extent of the normalized variable fluorescence due to the second (and subsequent) flash(es) declines in all sample types with a biphasic time dependence at longer illumination. The decay times of the fast (6-9 min) and the slow degradation component (60-75 min) are practically independent of the absence or presence of K3[Fe(CN)6] and of anaerobic and aerobic conditions during the photo-inhibitory treatment, while the relative extent of the fast decay component is higher under anaerobic conditions. (d) The relaxation kinetics of the variable fluorescence induced by the second (and subsequent) flash(es) become retarded due to photoinhibition, and (e) the oscillation pattern of the oxygen yield caused by a flash train is not drastically changed due to photoinhibition.Based on these findings, it is concluded that photoinhibition modifies the reaction pattern of the PS II acceptor side prior to protein degradation. The endogenous high spin Fe(2+) located between QA and QB is shown to become highly susceptible to modification by photoinhibition in the presence of K3[Fe(CN)6] (and other exogenous acceptors), while the rate constant of QA (-) reoxidation by QB(QB (-)) and other acceptors (except the special reaction via Fe(3+)) is markedly less affected by a short photoinhibition. The equilibrium constant between QA (-) and QB(QB (-)) is not drastically changed as reflected by the damping parameters of the oscillation pattern of oxygen evolution.

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Section: Resultssupporting

confidence: 84%

Effects of photoinhibition on the PS II acceptor side including the endogenous high spin Fe2+ in thylakoids, PS II-membrane fragments and PS II core complexes

1992

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“…at a molecular ratio of 15 quinone molecules per PS II in the reconstitution assay, all compounds other than PQ-9 are much less efficient or even without any effect. This idea is in line with previous failures to enhance the acceptor pool capacity with p-benzoquinone derivatives (Renger et al, 1989). Except for PQ-9, under the reconstitution conditions used here, the best results were obtained with DMDBQ, whereas UQ-9 gave rise to only marginal effects and Ph-p-BQ was totally inefficient.…”

Section: Discussionsupporting

confidence: 90%

“…On the other hand, a partial effect was obtained with DMDBQ. These findings provide convincing evidence for a comparatively high structural selectivity and readily explain our previous failure to achieve a significant increase of the pool size by using quiñones other than PQ-9 (Renger et al, 1989). If one considers the properties of the different quiñones studied, the present results suggest that the structure of the head group seems to be more important than the length of the hydrophobic tail and/or the general lipophilicity of the compound (see Discussion).…”

Section: Resultssupporting

confidence: 85%

“…It is known that several PS II complexes share a common PQ pool in thylakoids (Siggel et al, 1972). An analogous feature was observed in PS II membrane fragments (Renger et al, 1989). This phenomenon was ascribed to a "lake" of PQ molecules that can react with a large number of PS II complexes and permit a diffusionlimited functional connection with the cytochrome bfjf complex [see Haehnel (1984) and Cramer et al (1991)].…”

Section: Discussionmentioning

confidence: 83%

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Reconstitution of the Endogenous Plastoquinone Pool in Photosystem II (PS II) Membrane Fragments, Inside-Out-Vesicles, and PS II Core Complexes from Spinach

Kurreck¹,

Seeliger²,

Reifarth³

et al. 1995

Biochemistry

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The possibility of reconstituting a functionally competent endogenous plastoquinone pool in photosystem II (PS II) membrane fragments, inside-out-vesicles (ISO-vesicles), and PS II core complexes was analyzed by measuring (i) the characteristic period four oscillation of the oxygen yield due to excitation of dark-adapted samples with a train of short flashes and (ii) laser flash-induced transients of the relative quantum yield of chlorophyll fluorescence. The data obtained revealed that (a) an endogenous pool capacity comparable to that of intact thylakoids can be restored in PS II membrane fragments and ISO-vesicles by a sonication treatment using native plastoquinone-9, (b) a more pronounced oxygen oscillation pattern arises in PS II core complexes after application of the same reconstitution procedure, (c) the extent of the endogenous pool restoration at a ratio of 15 quinone molecules per PS II in the reconstitution assay strongly depends on the nature of the quinone molecule [maximum effects can be only achieved with PQ-9, while at the same concentration ubiquinone-45 (UQ-9) is almost inefficient], and (d) a sonication step is required for stable insertion of PQ-9 into PS II preparations. Measurements of the reconstruction degree as a function of the structure of different quinones with selected properties lead to the conclusion that specific binding domains exist in PS II in addition to the QB site. These domains exhibit a surprisingly high specificity for the type of quinone that can be bound. On the basis of a comparison of the results obtained, the structure of the quinone head group seems to be more important than the large hydrophobic side chain and/or the general lipophilicity of the compound.

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“…Since it was not possible to enrich the BBY with the tested commercial quinones (Renger et al 1989b), JM isolated plastoquinone (PQ) from spinach leaves and enriched the BBY samples with PQ by a short ultrasound treatment. This project was continued by Jens Kurreck and Angelika Seeliger (Kurreck et al 1995;Seeliger et al 1997).…”

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confidence: 99%

Gernot Renger (1937–2013): his life, Max-Volmer Laboratory, and photosynthesis research

2016

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Gernot Renger (October 23, 1937-January 12, 2013), one of the leading biophysicists in the field of photosynthesis research, studied and worked at the Max-Volmer-Institute (MVI) of the Technische Universität Berlin, Germany, for more than 50 years, and thus witnessed the rise and decline of photosynthesis research at this institute, which at its prime was one of the leading centers in this field. We present a tribute to Gernot Renger's work and life in the context of the history of photosynthesis research of that period, with special focus on the MVI. Gernot will be remembered for his thought-provoking questions and his boundless enthusiasm for science.

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Tribromotoluquinone Induced Modifications of the Oscillation Pattern of Oxygen Evolution and of Herbicide Binding in Thylakoids and PS II Membrane Fragments from Spinach

Cited by 10 publications

References 11 publications

Effects of photoinhibition on the PS II acceptor side including the endogenous high spin Fe2+ in thylakoids, PS II-membrane fragments and PS II core complexes

Effects of photoinhibition on the PS II acceptor side including the endogenous high spin Fe2+ in thylakoids, PS II-membrane fragments and PS II core complexes

Reconstitution of the Endogenous Plastoquinone Pool in Photosystem II (PS II) Membrane Fragments, Inside-Out-Vesicles, and PS II Core Complexes from Spinach

Gernot Renger (1937–2013): his life, Max-Volmer Laboratory, and photosynthesis research

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