Triboelectric effect to harness fluid flow energy

Haque, Rubaiyet Iftekharul; Arafat, Amr A.; Briand, D.

doi:10.1088/1742-6596/1407/1/012084

Cited by 13 publications

(17 citation statements)

References 10 publications

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“…This charge, accordingly, induces an electric field, which affects the protein properties. Such an effect can be observed for both water (and aqueous solutions [34][35][36][37]) and non-aqueous liquids [6,38]. We also demonstrated that the properties of a protein in a solution are affected by the flow of water flow through a coiled pipe [8].…”

Section: Discussionmentioning

confidence: 56%

AFM Study of the Influence of Glycerol Flow on Horseradish Peroxidase near the in/out Linear Sections of a Coil

et al. 2021

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Flow-based coiled systems, through which a heat transfer fluid (such as glycerol) is pumped, are widely used for thermal stabilization of bioreactors and biosensor cuvettes and cells. Previously, using horseradish peroxidase (HRP) as a model protein, we have demonstrated that the incubation of a protein solution in a flow-based system over coiled pipe with flowing glycerol leads to a change in the adsorption properties of the protein macromolecules. Herein, we have studied the effect of the glycerol flow on the properties of HRP, the solution of which was placed differently: i.e., near either the inflow or the outflow linear sections of the pipe, while the coiled section of the pipe was shielded with a grounded metallic cover. Atomic force microscopy (AFM) has been employed in order to visualize the HRP protein macromolecules adsorbed from its solution onto the mica substrate surface. The quantity of adsorbed protein was estimated based on the AFM data. The enzymatic activity of HRP was estimated by spectrophotometry. We demonstrate that a change in the properties of HRP enzyme was observed after the incubation of its solution near the inflow/outflow linear sections of the pipe with flowing glycerol. Namely, after the incubation of HRP solution near the inflow section, a decrease in the protein adsorption onto mica was observed, but its enzymatic activity remained unchanged in comparison to the control sample. In another case, when the HRP solution was incubated near the outflow section, an increased protein adsorption was observed, while the enzyme exhibited considerably lower activity.

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Section: Discussionmentioning

confidence: 56%

AFM Study of the Influence of Glycerol Flow on Horseradish Peroxidase near the in/out Linear Sections of a Coil

et al. 2021

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“…For these purposes, aqueous or non-aqueous media can be employed, depending on the process's design. In connection with this, in a number of papers, an occurrence of a triboelectric generation of charge upon the motion of liquid media along solid surfaces was demonstrated [5][6][7][8][9][10][11][12]. Such a triboelectric effect is known to occur upon the motion of both water and aqueous solutions [5][6][7][8][9][10][11][12], as well as of various non-aqueous liquids [13,14], including glycerol [14].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow

et al. 2020

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Atomic force microscopy is employed to study the influence of the motion of a glycerol solution through a coiled (spiral-wound) polymeric communication pipe on the aggregation state of a protein, with the example of a horseradish peroxidase (HRP) enzyme. The measuring cell with the buffered solution of the protein was placed within the experimental setup over the pipe coil, through which glycerol was pumped. It is demonstrated that, in such a system, the flow of a non-aqueous liquid (glycerol) leads to a change in the physicochemical properties of a protein, whose solution was incubated in the measuring cell placed over the coil. Namely, changes in both the adsorbability onto mica and the aggregation state of the model HRP protein were observed. As glycerol-containing liquids are commonly used in biosensor operations, the results reported herein can be useful to the development of biosensor systems, in which polymeric communications are employed in sample delivery and thermal stabilization systems. The data obtained herein can also be of use for the development of specified hydrodynamic models.

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“…The observed effect of the water, flowing through the coiled silicone pipe, on the adsorbability of HRP is apparently explained by the electromagnetic nature of the flow-induced field. The induction of such an electromagnetic field obviously occurs owing to a triboelectric effect consisting of a generation of charge in the liquid, flowing along a polymeric surface [ 8 , 29 , 30 , 31 , 32 , 33 , 34 ]. The triboelectrically induced charges induce an electromagnetic field, which, in turn, affects the protein during the incubation of its solution near the coil.…”

Section: Discussionmentioning

confidence: 99%

AFM and FTIR Investigation of the Effect of Water Flow on Horseradish Peroxidase

et al. 2021

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Atomic force microscopy (AFM)-based fishing is a promising method for the detection of low-abundant proteins. This method is based on the capturing of the target proteins from the analyzed solution onto a solid substrate, with subsequent counting of the captured protein molecules on the substrate surface by AFM. Protein adsorption onto the substrate surface represents one of the key factors determining the capturing efficiency. Accordingly, studying the factors influencing the protein adsorbability onto the substrate surface represents an actual direction in biomedical research. Herein, the influence of water motion in a flow-based system on the protein adsorbability and on its enzymatic activity has been studied with an example of horseradish peroxidase (HRP) enzyme by AFM, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and conventional spectrophotometry. In the experiments, HRP solution was incubated in a setup modeling the flow section of a biosensor communication. The measuring cell with the protein solution was placed near a coiled silicone pipe, through which water was pumped. The adsorbability of the protein onto the surface of the mica substrate has been studied by AFM. It has been demonstrated that incubation of the HRP solution near the coiled silicone pipe with flowing water leads to an increase in its adsorbability onto mica. This is accompanied by a change in the enzyme’s secondary structure, as has been revealed by ATR-FTIR. At the same time, its enzymatic activity remains unchanged. The results reported herein can be useful in the development of models describing the influence of liquid flow on the properties of enzymes and other proteins. The latter is particularly important for the development of biosensors for biomedical applications—particularly for serological analysis, which is intended for the early diagnosis of various types of cancer and infectious diseases. Our results should also be taken into account in studies of the effects of protein aggregation on hemodynamics, which plays a key role in human body functioning.

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Triboelectric effect to harness fluid flow energy

Cited by 13 publications

References 10 publications

AFM Study of the Influence of Glycerol Flow on Horseradish Peroxidase near the in/out Linear Sections of a Coil

AFM Study of the Influence of Glycerol Flow on Horseradish Peroxidase near the in/out Linear Sections of a Coil

Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow

AFM and FTIR Investigation of the Effect of Water Flow on Horseradish Peroxidase

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