Tribocorrosion studies on diamond-like carbon film deposited by PECVD on 304 stainless steel in simulated body fluid

Abstract

In this paper, a diamond-like carbon (DLC) film with a controlled content of hydrogen (20%) and carbon (80%) was deposited onto a 304 stainless steel (304SS). Tribocorrosion tests were performed following immersion in simulated body fluid (SBF) solution. The DLC film with hydrogen and carbon control decreased the SS304 corrosion susceptibility 59-fold, compared to the bare surface, improving on the corrosion protection provided by DLC reported in the literature. Debris or nanoparticles from corroded metals can cause cytotoxicity or genotoxicity towards many cells, so these possible effects were evaluated using A172 glial cells (ATCC #CRL-1620 glioblastoma). The DLC film was not genotoxic or cytotoxic towards the glial cells. Furthermore, no cytotoxicity or genotoxicity was observed in the A172 cells after exposure to the SS304 covered with DLC film, even after 24 h of incubation, when compared to untreated cells. The methodology adopted, using a silicon interface between the DLC and the 304SS, was effective in preventing delamination of the DLC film. This report provides full information concerning methods for the production and characterisation of this DLC film.

Diamond Deposition on Iron and Steel Substrates: A Review

“…Doped DLC films have been prepared and studied, such as B-doped [84,85], Ti-doped [81], Ag-doped [86,87], Ge-doped [88] and so on. These special doped DLC films are ideal protection for the biomedical alloys from oxidation, passivation and to reduce the ability for a bacterial biofilm to form after implantation [89]. Ag-containing DLC films were prepared on 316 steel substrates by magnetron sputtering at different silver target currents, which showed a long-lasting and reusable antibacterial activity through the antibacterial tests of Escherichia coli, attributed to the Ag particles distributed in the films [86,87].…”

Wear Rate, Tribo-Corrosion, and Plastic Deformation Values of Co-Cr-Mo Alloy in Ringer Lactate Solution

“…Sample A4, with grain sizes comparable to A3, also showed similar resilience during the rubbing phase, underscoring the importance of microstructural optimization in mitigating corrosion. Oliveira et al [57] further corroborated these findings, indicating that a potential reduction often signals heightened corrosion susceptibility.…”

Structural, biocompatibility, and antibacterial properties of Ge–DLC nanocomposite for biomedical applications

“…To monitor nuclear abnormalities, Hoechst 33258 fluorescent staining was performed and cellular structures were investigated under a never fluorescent microscope. Although there were not much information about DLC and genotoxicity relationship in literature, a tribocorrosion study on DLC deposited stainless steel claimed that debris of DLC film did not stimulate genotoxicological features on A172 glial cells 45 . Also, several studies proposed that Ge based alloys and molecules did not showed genotoxic and mutagenic properties on bacterial mutagenicity tests ( Salmonella typhimurium and Escherichia coli ), and also on animal studies (mouse bone marrow micronucleus test and sperm abnormality assay) 46–48 .…”

“…Although there were not much information about DLC and genotoxicity relationship in literature, a tribocorrosion study on DLC deposited stainless steel claimed that debris of DLC film did not stimulate genotoxicological features on A172 glial cells. 45 Also, several . [46][47][48] In the present study, genotoxic analysis of DLC-Ge surface investigated on human fibroblast cell culture showed that there was no significant nuclear abnormality (NA) rate on the coated surface (0.023 NA/1000 cells) compared to the (À) control (0.024 NA/1000 cells).…”