Tribes and Clades Within Apiaceae Subfamily Apioideae: The Contribution of Molecular Data

Abstract

Phylogenetic analyses of chloroplast gene (rbcL, matK ), intron (rpl16, rps16, rpoC1) and nuclear ribosomal DNA internal transcribed spacer (ITS) sequences and chloroplast DNA restriction sites, with supplementary data from variation in size of the chloroplast genome inverted repeat, have been used to elucidate major clades within Apiaceae (Umbelliferae) subfamily Apioideae Drude. This paper summarizes the results of previously published molecular cladistic analyses and presents a provisional classification of the subfamily based on taxonomic congruence among the data sets.Boiss., Scandiceae Spreng. and Smyrnieae Spreng.) are erected or confirmed as monophyletic, with Scandiceae comprising subtribes Daucinae Dumort., Scandicinae Tausch and Torilidinae Dumort. Seven additional clades are also recognized but have yet to be treated formally, and at least 23 genera examined to date are of dubious tribal or clade placement. The utility of these different molecular markers for phylogenetic inference in Apioideae is compared based on maximum parsimony analyses of subsets of previously published molecular data sets. Of the six loci sequenced, the ITS region is seen to be evolving most rapidly and rbcL is the most conservative. Intermediate in rate of evolution are matK and the three chloroplast introns; with rpl16 and rps16 evolving slightly faster than matK or rpoC1. The analysis of restriction sites, however, provided 2-4 times more parsimony informative characters than any single DNA locus sequenced, with estimates of divergence just slightly lower than that of the ITS region. The trees obtained from separate analyses of these reduced data sets are consistent with regard to the major clades inferred and the relationships among them. Similar phylogenies are obtained by combining data or combining trees, representing the supermatrix and supertree approaches to

Molecular evidence of polyphyletism in the plant genus Carum L. (Apiaceae)

“…tribe (also called the Aegopodium clade after Downie et al, 2001), as previously observed by other authors. We also found that C. heldreichii, C. appuanum and H. multiflorum clustered within the Pyramidoptereae Boiss.…”

“…We also found that C. heldreichii, C. appuanum and H. multiflorum clustered within the Pyramidoptereae Boiss. tribe (the Crithmum group in Downie et al, 2001). The first two species formed a well supported clade with 96% bootstrapping and 100% Bayesian support.…”

“…We used the tribal nomenclature after Downie et al (2001) particularly for the Careae Baill. and Pyramidoptereae Boiss.…”

Evaluation of the taxonomic status of water dropwort (Oenanthe, Apiaceae) accessions from East Asia based on nuclear rDNA internal transcribed spacer sequences

“…To date, many gene markers have been employed in the phylogenetic study of the Apiaceae (Downie et al, 2001Kadereit and Kadereit, 2005;Zhou et al, 2008Zhou et al, , 2009Spalik et al, 2009Spalik et al, , 2014, such as nrDNA ITS region, chloroplast DNA gene (rbcL and matK), intergenic spacer (psbI-5'trnK and rps16-5'trnK), and intron (rpl16, rps16, and rpoC1) sequences. Among these, the ITS region is the most frequently used marker.…”

“…The ITS region has also been successfully used for low-level phylogenetic analyses in the Apiaceae (Downie et al, 2001Kadereit and Kadereit, 2005;Zhou et al, 2008Zhou et al, , 2009Spalik et al, 2009). The numerous ITS sequences available in GenBank for almost all currently reported species of Oenanthe provide a reference for species discrimination.…”