Triamcinolone Suppresses Human Tenocyte Cellular Activity and Collagen Synthesis

Wong, Margaret Wan Nar; Tang, Yin Nei; Fu, Sai‐Chuen; Lee, Wayne; Chan, Kai Ming

doi:10.1097/01.blo.0000118184.83983.65

Cited by 102 publications

(70 citation statements)

References 32 publications

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“…There are a number of choices for injectable steroids, and all have potential side effects [20,26,39]. Triamcinolone was available in our institution.…”

Section: Discussionmentioning

confidence: 99%

Prospective Comparison of the Six-Item Carpal Tunnel Symptoms Scale and Portable Nerve Conduction Testing in Measuring the Outcomes of Treatment of Carpal Tunnel Syndrome with Steroid Injection

Craw

Church

Hutchison

2014

Hand (New York, N,Y.)

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Background We prospectively studied patients clinically diagnosed with carpal tunnel syndrome (CTS) and treated with the injection of corticosteroid into their carpal tunnel in order to compare changes in the six-item CTS symptoms scale and portable nerve conduction study (NCS) parameters as outcome tools. Our pilot study was the first to assess the utility of the six-item CTS symptom scale (CTS-6) with steroid injections as a patient-directed outcome measure for the treatment of CTS. Methods We enrolled patients who presented to our county hospital orthopedic surgery clinic from August 2012 through August 2013. The patients were clinically diagnosed with CTS. After completing the six-item CTS symptoms scale questionnaire, portable NCS was obtained. Each patient then received an injection of 1 ml of triamcinolone acetonide 40 mg/ml mixed with 1 ml of 1 % lidocaine into the carpal tunnel. Six weeks postinjection, each patient repeated a CTS-6 questionnaire and underwent a repeat portable NCS. The CTS-6 and NCS results were analyzed using the paired samples t test. A Pearson correlation was used to assess the correlation between the changes in the CTS-6 and the NCS measurements. Statistical significance was set at P<0.05. Results Thirty-two wrists in 20 patients were evaluated. There was a statistically significant difference between the CTS-6 scores before and after injection. There were also statistically significant changes in the five of the NCS parameters. None of the correlations between the CTS-6 and the NCS parameters were statistically significant. Conclusions The six-item CTS symptoms scale and portable NCS are both useful measures for evaluating the results of steroid injections. The CTS-6 is an effective tool because of its ease of use, low cost, correspondence with changes in NCS, and ability to monitor the outcome of steroid treatment for carpal tunnel syndrome.

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“…There are a number of choices for injectable steroids, and all have potential side effects [20,26,39]. Triamcinolone was available in our institution.…”

Section: Discussionmentioning

confidence: 99%

Prospective Comparison of the Six-Item Carpal Tunnel Symptoms Scale and Portable Nerve Conduction Testing in Measuring the Outcomes of Treatment of Carpal Tunnel Syndrome with Steroid Injection

Craw

Church

Hutchison

2014

Hand (New York, N,Y.)

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“…In vitro studies revealed degenerative changes, reduced synthesis of collagen type I and proteoglycans, as well as decreased cell proliferation, viability and metabolism following glucocorticoid exposure [26][27][28][29][30]. Experimental evidence on the effects of steroids in combination with quinolones is even more limited.…”

Section: Page 5 Of 37mentioning

confidence: 99%

Synergistic effects of dexamethasone and quinolones on human-derived tendon cells

Sendzik

Shakibaei

Schäfer‐Korting

et al. 2010

International Journal of Antimicrobial Agents

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Quinolones and glucocorticoids are frequently used drugs that may cause tendinopathy as a rare adverse effect. We exposed human tenocyte cultures to the steroid dexamethasone alone or in combination with either ciprofloxacin or levofloxacin at concentrations of 3 mg/L and 10 mg/L. At concentrations corresponding to peak levels in plasma and tissues during therapy (ca. 3-10 mg/L), ciprofloxacin caused a significant decrease in collagen type I and the 1 -integrin receptor. In contrast, no corresponding effect was induced by 3 mg/L levofloxacin.With both quinolones at 3 mg/L and 10 mg/L, the amount of matrix metalloproteinase-1 (MMP-1) and MMP-13 was increased. In addition, 3 mg/L ciprofloxacin and 10 mg/L levofloxacin activated caspase-3. Apoptotic changes were confirmed by electron microscopy. Incubation of human tenocytes with dexamethasone decreased the main matrix protein collagen type I, the

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“…31 Many studies have demonstrated that the administration of corticosteroids in cell cultures from tendons affect tenocyte viability, proliferation, collagen type I synthesis, and migration. 12,13,32,33 In the present study, we used a more similar model to the clinical situation, and instead of administering corticosteroids to cells in culture, we isolated tenocytes from the tendons of animals previously submitted to pharmacological therapy.…”

Section: Discussionmentioning

confidence: 99%

“…9 In the present study, cells derived from tendons were used and were characterized by means of cell morphology and ECM protein production as performed in many other studies. 12,13,21,23,26,29,30,33,35 Moreover, in our study in order to maintain the phenotype we used the explant technique, in which cells are permitted to grow from small fragments of tissue instead of enzymatic digestion that affects cell membrane and destroys the cell-cell and cell-matrix relationship. 36 It is reported that the explant technique is best to determine the phenotype of the cells derived from tendons.…”

Section: Discussionmentioning

confidence: 99%

“…12 However, cases of tendon rupture following local and systemic corticosteroid administration suggest that such treatment may impair the healing process and have a deleterious effect on tendon strength. 13 An in vitro study was performed to evaluate two de-cellularized collagen matrices already proposed for shoulder tendon healing: porcine small intestinal submucosa (SIS; DePuy Orthopaedics, Inc., Warsaw, IN), and human dermal matrix (Graftjacket 1 , GJ; Wright Medical Technology Inc., Arlington, TN). SIS has been reported to be an acellular collagen-based matrix primarily composed of more than 90% fibrillar collagen (type I, II, and V) and containing transforming growth factor b 1 (TGF-b1).…”

Section: Introductionmentioning

confidence: 99%

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In vitro study comparing two collageneous membranes in view of their clinical application for rotator cuff tendon regeneration

Fini

Torricelli

Giavaresi

et al. 2006

Journal Orthopaedic Research

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Tenocytes were isolated from the rotator cuff tendons of healthy (HT) and glucocorticoid (GC)-treated rats (GCT) and were cultured on polystyrene wells (TCP) as control, and on 2 de-cellularized collagen matrices: porcine small intestinal submucosa (SIS), and human dermal matrix (Graftjacket 1 , GJ). At 3 and 7 days cell proliferation and synthesis were evaluated. Proliferation of HT tenocytes increased between experimental times for both tested membranes, but already at 3 days, HT tenocytes cultured on GJ showed the highest WST-1 value. The collagen-I (CICP) synthesis on GJ membrane did not change between experimental times and was significantly higher than TCP and SIS at 7 days. Proteoglycans (PG), and fibronectin (FBN) synthesis increased when HT were cultured on GJ, between experimental times, and both PG and FBN synthesis on GJ membrane were higher than TCP and SIS at 7 days. GC determined decreases in cell proliferation, CICP and PG syntheses at 3 days of culture on TCP when compared to HT tenocytes while a decrease in WST-1 was maintained at 7 days. CICP, PG and FBN (only at 3 days) syntheses were significantly higher in GCT tenocytes cultured on GJ. The negative effects on GC on GCT tenocytes cultured on membrane were particularly evident on SIS for CICP (À18%) and FBN (À67%) synthesis. The obtained results support the conclusion that GJ is more suitable than SIS as a scaffold for in situ tissue engineering and for the in vitro bioengineering of tendons to heal massive tears of the rotator cuff tendon. ß

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Triamcinolone Suppresses Human Tenocyte Cellular Activity and Collagen Synthesis

Cited by 102 publications

References 32 publications

Prospective Comparison of the Six-Item Carpal Tunnel Symptoms Scale and Portable Nerve Conduction Testing in Measuring the Outcomes of Treatment of Carpal Tunnel Syndrome with Steroid Injection

Prospective Comparison of the Six-Item Carpal Tunnel Symptoms Scale and Portable Nerve Conduction Testing in Measuring the Outcomes of Treatment of Carpal Tunnel Syndrome with Steroid Injection

Synergistic effects of dexamethasone and quinolones on human-derived tendon cells

In vitro study comparing two collageneous membranes in view of their clinical application for rotator cuff tendon regeneration

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