Triamcinolone acetonide loaded-cationic nano-lipoidal formulation for uveitis: Evidences of improved biopharmaceutical performance and anti-inflammatory activity

Abstract

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Natural Ergot Alkaloids in Ocular Pharmacotherapy: Known Molecules for Novel Nanoparticle-Based Delivery Systems

“…The selection of ophthalmically acceptable excipients plays a vital role in successful design of functional and stable lipid nanoparticles. There is currently increased interest in promoting a positive charge onto lipid nanoparticles by coating them with a cationic moiety, e.g., chitosan [ 89 ], l -arginine [ 90 ], and/or other cationic lipids (cetyl trimethyl ammonium bromide and stearylamine) [ 91 ]. Stearylamine is a lipid with surface-modifying property that is commonly used to produce positively charged lipid nanoparticles.…”

Dexamethasone-Loaded Nanostructured Lipid Carriers for the Treatment of Dry Eye Disease

“…Similarly, Yu et al used coumarin-6 loaded NLCs in their investigation of the HCEC uptake of NLCs designed to deliver quercetin to the ocular surface, though the fluorescent material was there quantified after the cells were lysed [ 51 ]. In the present study, the focus was on a qualitative investigation of the presence of coumarin-6 loaded NLCs in HCECs when compared with untreated cells, in a method similar to that used by Nirbhavane et al where fluorescence was used to demonstrate HCEC internalization of NLCS [ 27 ]. Once internalized within the cell, as demonstrated in Figure 2 d it is hypothesized that the NLCs could release their therapeutic payload as enzyme cleavage in the endosomes, acid mediated degradation in the lysosomes, or both, would degrade the NLC components [ 29 , 52 , 53 , 54 , 55 ].…”

“…After 4 h of incubation at 37 °C, the medium was removed, and the cornea washed. Fluorescent microscopy under DIC and FITC modes of analysis was used to determine the corneal distribution [ 27 ].…”

Appraising the Competency of Lipid Nano-Platforms as Non-invasive Paradigms for the Treatment of Ocular Inflammations: A review

“…The ex vivo corneas isolated from animals were used to localize the designated fluorescently-labeled nanoparticles across the corneal epithelium and stroma using fluorescence or confocal laser microscopy (CLSM) [29], [60]. Younes et al [69] studied the in vivo corneal uptake by instilling the Rhodamine B-labeled nanoparticles into the rabbits" eyes, then the eyes were gently washed and the corneas were excised and fixed on glass slides for examination using CLSM.…”