Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid

Warda, Safaa A.; Abd-Elaziz, Wafaa R.; Ebied, E. M.; Madkour, Nashwa K.; Sawy, Hala El; Warda, Fatma F.; Al-Shamandy, Omaima A.A.; Kotb, Attyat M.

doi:10.21608/javs.2021.140096

Cited by 1 publication

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“…However, the technology for the use of these vaccines provides for two and three times immunization. [26,27,28]. Vaccines are not registered in the Russian Federation, except for the "Pneumequin" vaccine [21].…”

Section: Introductionmentioning

confidence: 99%

Preclinical tests of Vaccine against Rhinopneumonia and Strangles of young horses

2022

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Efficacy and safety of the combined vaccine against rhinopneumonia and strangles of horses were evaluated on outbred mice and rabbits. Acute toxicity, pyrogenicity and immunogenicity were carried out in accordance with the methods regulated by the Rosselkhoznadzor of the Russian FederationThe absence of toxicity was established with a single intragastric administration of the vaccine to white mice. An analysis of the dynamics of the body weight of white mice revealed an increase in both the control and experimental groups. Thus, the increase was 9.2% in the experimental group that received the vaccine preparation, and in the control group -5.05%. Therefore, in mice treated intragastrically with the vaccine preparation, active growth is observed in comparison with the control. The difference was 4.15%. The disease and mortality among vaccinated mice were not noted, which indicates its harmlessness and safety.The test of the vaccine preparation on rabbits showed its non-pyrogenicity. In the third hour after the administration of the drug, the temperature of rabbits in the control group rose by 0.2ºC, and in the experimental group by 0.4ºC. However, during a clinical examination of the experimental animals of both groups, signs of illness and changes in the general condition were not recorded. Thus, the studied vaccine preparation was considered non-pyrogenic, since after its administration to rabbits, a slight short-term increase in body temperature is observed in animals of both groups, in which the general condition of the animals remains favorable.The immunogenicity of the viral component of the associated vaccine against rhinopneumonia and strangles of horses was studied on outbred mice. Studies have shown sufficient immunogenicity (75%) when the vaccine is administered subcutaneously at a dose of 0.3 cm3 twice with an interval of 14 days, followed by control infection with an adapted strain of equine rhinopneumonia virus at a dose of 0.02x6.0 lgTCD50/ml intracerebral.Evaluation of the immunogenic activity of the strangles composition of the vaccine was carried out in experiments with a single immunization of white outbred mice, followed by infection with a virulent culture of the strangles streptococcus Streptococcus equi "H-5/1" at a dose of LD50 (200 thousand microbial bodies per 1 cm3). The bacterial component protects against the causative agent of strangles by 80% of the number of infected animals.The aim of our research was to study the efficacy and safety of the combined vaccine against rhinopneumonia and strangles of horses in laboratory conditions. This paper presents the results of preclinical studies on laboratory animals. The absence of acute toxicity, allergenic, pyrogenic properties and the safety of using the associated vaccine against rhinopneumonia and strangles of horses on laboratory animals have been proven.

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Section: Introductionmentioning

confidence: 99%

Preclinical tests of Vaccine against Rhinopneumonia and Strangles of young horses

2022

pnr

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show abstract

Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid

Cited by 1 publication

References 7 publications

Preclinical tests of Vaccine against Rhinopneumonia and Strangles of young horses

Preclinical tests of Vaccine against Rhinopneumonia and Strangles of young horses

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