Triacylglycerol Analysis in Human Milk and Other Mammalian Species: Small-Scale Sample Preparation, Characterization, and Statistical Classification Using HPLC-ELSD Profiles

Ten-Doménech, Isabel; Beltrán-Iturat, Eduardo; Herrero‐Martínez, José Manuel; Sancho, Juan V.; Simó‐Alfonso, Ernesto Francisco

doi:10.1021/acs.jafc.5b01158

Cited by 49 publications

(48 citation statements)

References 49 publications

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“…44 Outros métodos de extração, baseados em Folch et al (1957) e em Bligh e Dyer (1959), foram desenvolvidos posteriormente, com o intuito de substituir e reduzir o volume dos solventes tóxicos, como o clorofórmio, além de aumentar o rendimento da extração e otimizar o tempo de análise. 45,46 Uma destas técnicas utiliza o éter metil-terc-butilílico, baseando-se também na separação de fases e tem sido utilizada para a análise de lipídios contidos no LH. 22 A extração em fase sólida (SPE) também pode ser utilizada na extração de diferentes classes de lipídios, utilizando-se cartuchos contendo uma fase sólida para a extração dos lipídios, que são posteriormente eluidos com solventes orgânicos adequados, enquanto os interferentes permanecem retidos na fase sólida, promovendo a separação das fases de interesse.…”

Section: Extração E Posterior Dosagem Lipídica Do Leite Humanounclassified

“…No entanto, este último gradiente pode levar a baixa resolução dos picos de TAGs. 46 Garwolińska et al (2018) utilizaram HPLC acoplada a MS (HPLC-MS) para a caracterização de glicerofosfolipídios, glicerolipídios, esfingolipídios e esteróis, e mostraram que o método utilizado é rápido, livre de solventes tóxicos e adequado para a análise qualitativa da composição lipídica de amostras de LH. 15 A HPLC-MS é uma técnica que pode ser utilizada para a caracterização e quantificação de fosfolipídios do LH, podendo estes serem separados por uma coluna C18 em classes como fosfatidilinositol, fosfatidiletanolamina, fosfatidilserina, fosfatidilcolina e esfingomielina.…”

Section: Cromatogra Ia Líquida (Lc)unclassified

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Analytical Methods Used for the Determination of Lipids in Human Milk: A Review

Rydlewski¹,

Pizzo²,

Manin³

et al. 2020

Rev. Virtual Quim.

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Human milk is considered the ideal and most complete food for the neonate, due to its balanced nutritional composition, which contributes to the full physical and neurological development of the infant. The lipids present in human milk are sources of saturated, monounsaturated and polyunsaturated fatty acids. The polyunsaturated fatty acids of the omega-6 series, such as linoleic acid and omega-3 series, such as α-linolenic acid, are essential and are also precursors of long-chain polyunsaturated fatty acids, such as arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid, which are involved in a number of functions, such as the development of the nervous system during childhood and the increase in the intelligence quotient of the infant. Due to the lipid importance of human milk, the aim of this review article was to present the analytical methods that are currently being employed to determine its lipid composition. There are few scientific advances in lipid extraction methods and to date, most studies have evaluated the composition of fatty acids by gas chromatography. Liquid chromatography and mass spectrometry have been widely used to identify and quantify different classes of lipids of human milk. O leite humano é considerado o alimento ideal e mais completo para o neonato, devido à sua composição nutricional equilibrada, contribuindo para o pleno desenvolvimento físico e neurológico do lactente. Os lipídios presentes no leite humano são fontes de ácidos graxos saturados, monoinsaturados e poliinsaturados. Os ácidos graxos poli-insaturados da série ômega-6, como o ácido linoleico e da série ômega-3, como o α-linolênico, são essenciais e também são precursores de ácidos graxos poli-insaturados de cadeia longa, como o ácido araquidônico, ácido eicosapentaenoico e ácido docosahexaenoico, que estão envolvidos no desenvolvimento do sistema nervoso e no aumento do quociente de inteligência do lactente. Devido à importância lipídica do leite humano, o objetivo deste artigo de revisão foi apresentar os métodos analíticos que têm sido atualmente mais empregados para determinar sua composição lipídica. Há poucos avanços científicos quanto aos métodos de extração lipídica e até o presente momento, grande parte dos estudos avaliaram, a composição em ácidos graxos, a partir de cromatografia em fase gasosa. A cromatografia líquida e a espectrometria de massas têm sido utilizadas para identificação e quantificação de distintas classes lipídicas presentes no leite humano.

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Section: Extração E Posterior Dosagem Lipídica Do Leite Humanounclassified

Section: Cromatogra Ia Líquida (Lc)unclassified

Analytical Methods Used for the Determination of Lipids in Human Milk: A Review

Rydlewski¹,

Pizzo²,

Manin³

et al. 2020

Rev. Virtual Quim.

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“…After the acquisition, Bruker raw data .d files were automatically calibrated using the internal calibration and converted into mzXML format using a custom DataAnalysis script (Bruker, Version 4.3). The mzXML files were then subjected to the standard alignment and peak picking procedure using xcms software [21]. We then filtered from the output table all lipid features with the coefficient of variation (CoV, calculated as the standard deviation over the mean across QC samples) > 30%, and peaks with zero values in > 50% of the individual samples.…”

Section: Data Preprocessingmentioning

confidence: 99%

“…TAGs have simple chemical composition, consisting of glycerol and three fatty acid residues. Several studies analyzed milk TAG composition in mammalian species, including humans [17][18][19][20][21], demonstrating differences in the distribution of docosahexaenoic acid (DHA)-containing TAGs in comparison with the baby formula [22]. However, the systematic characterization of the milk lipidome composition across mammalian species remained incomplete.…”

mentioning

confidence: 99%

Lipidome analysis of milk composition in humans, monkeys, bovids, and pigs.

Mitina

Mazin

Vanyushkina

et al. 2020

Preprint

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Background Lipids contained in milk are an essential source of energy and structural materials for a growing infant. Furthermore, lipids' long-chain unsaturated fatty acid residues can directly participate in infant tissue formation. Here, we used untargeted mass spectrometric measurements to assess milk lipid composition in seven mammalian species: humans, two macaque species, cows, goats, yaks, and pigs.Results Analysis of the main milk lipid class, triacylglycerides, revealed species-specific quantitative differences in the composition of fatty acid residues for each of seven species. Overall, differences in milk lipid composition reflect evolutionary distances among species, with each species group demonstrating specific lipidome features. Among them, human milk contained more medium and long-chain unsaturated fatty acids compared to other species, while pig milk was the most distinct, featuring the highest proportion of long-chain polyunsaturated fatty acids. ConclusionsWe show that milk lipidome composition is dynamic across mammalian species, changed extensively in pigs, and contains features particular to humans.

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“…TAGs profile is also important for food of animal origin, and the possibility of classifying milks according to their mammalian origin by using this parameter obtained by HPLC-ELSD has been explored by Ten-Doménech and coauthors and a high throughput method employing a C18 core-shell column was developed [ 45 ].…”

Section: Applications In Food Analysismentioning

confidence: 99%

Core-Shell Columns in High-Performance Liquid Chromatography: Food Analysis Applications

Preti

2016

International Journal of Analytical Chemistry

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The increased separation efficiency provided by the new technology of column packed with core-shell particles in high-performance liquid chromatography (HPLC) has resulted in their widespread diffusion in several analytical fields: from pharmaceutical, biological, environmental, and toxicological. The present paper presents their most recent applications in food analysis. Their use has proved to be particularly advantageous for the determination of compounds at trace levels or when a large amount of samples must be analyzed fast using reliable and solvent-saving apparatus. The literature hereby described shows how the outstanding performances provided by core-shell particles column on a traditional HPLC instruments are comparable to those obtained with a costly UHPLC instrumentation, making this novel column a promising key tool in food analysis.

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Triacylglycerol Analysis in Human Milk and Other Mammalian Species: Small-Scale Sample Preparation, Characterization, and Statistical Classification Using HPLC-ELSD Profiles

Cited by 49 publications

References 49 publications

Analytical Methods Used for the Determination of Lipids in Human Milk: A Review

Analytical Methods Used for the Determination of Lipids in Human Milk: A Review

Lipidome analysis of milk composition in humans, monkeys, bovids, and pigs.

Core-Shell Columns in High-Performance Liquid Chromatography: Food Analysis Applications

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