Tri-n-butyltin-induced cytotoxicity on rat thymocytes in presence and absence of serum

Umebayashi, Chisato; Oyama, Yasuo; Chikahisa-Muramastu, L; Nakao, Hiromi; Nishizaki, Yasutaka; Nakata, Mami; Soeda, Fumio; Takahama, Kazuo

doi:10.1016/j.tiv.2003.08.003

Cited by 19 publications

(13 citation statements)

References 25 publications

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“…Several studies demonstrated that TBT affects the viability of a wide range of normal and tumour cells [13][14][15][16]. To examine the effect of TBT on EoL-1 cell viability, in the first series of experiments, the cells were incubated with TBT in concentrations ranging from 6.25 to 100 nM for 24 hours, and the percentage of viable cells was calculated.…”

Section: Resultsmentioning

confidence: 99%

The effect of tributyltin on human eosinophylic leukemia EoL-1 cells

Sroka

Włosiak

Wilk

et al. 2008

Cellular and Molecular Biology Letters

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Organotin compounds are chemicals that are widely used in industry and agriculture as plastic stabilizers, catalysts and biocides. Many of them, including tributyltin (TBT), have been detected in human food and, as a consequence, detectable levels have been found in human blood. As organotin compounds were shown to possess immunotoxic activity, we focused our attention on the effect of TBT on the basic determinants of the function of eosinophils, i.e. cell adhesiveness and motility. We used human eosinophylic leukemia EoL-1 cells, a common in vitro cellular model of human eosinophils. Here, we demonstrate that TBT causes a dose-dependent decrease in the viability of EoL-1 cells. When administered at sub-lethal concentrations, TBT significantly decreases the adhesion of EoL-1 cells to human fibroblasts (HSFs) and inhibits their migration on fibroblast surfaces. Since the basic function of eosinophils is to invade inflamed tissues, our results indicate that TBT, and possibly other organotin compounds, may affect major cellular properties involved in the determination of in vivo eosinophil function.

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Section: Resultsmentioning

confidence: 99%

The effect of tributyltin on human eosinophylic leukemia EoL-1 cells

Sroka

Włosiak

Wilk

et al. 2008

Cellular and Molecular Biology Letters

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“…17) The cells were incubated with this solution for 1 hr at room temperature. Thereafter, the propidium fluorescence was measured at 600 ± 20 nm by using the flow cytometer.…”

Section: Isolation Of Cytotoxic Dimmers --Oxidation Ofmentioning

confidence: 99%

Cytotoxic Characteristics of Two Isomeric Dimers Produced by Oxidation of Sesamol, an Antioxidant in Sesame Oil

Shingai

Fujimoto

Nakajima

et al. 2011

JOURNAL OF HEALTH SCIENCE

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Antioxidants themselves are oxidized to prevent oxidation of other molecules. One may ask if the oxidized antioxidants are safe for humans. However, there is very little information on the toxicity of oxidized antioxidants. We previously identified cytotoxic compounds in the products from oxidation of sesamol, a potent antioxidant in sesame oil. In this study using a flow cytometer with fluorescent probes, we revealed cytotoxic characteristics of two isomeric dimers (dimer A and B) in rat thymocytes. Increase in cell lethality by dimer A was more profound than those by sesamol and dimer B. The incubation of cells with dimer A increased the populations of shrunken cells and the cells with phosphatidylserine exposed on outer surface of cell membranes. Since these phenomena are parameters for early stage of apoptosis, the results indicate that dimer A may promote the process of apoptosis. However, the population of the cells containing hypodiploid DNA, a parameter for late stage of apoptosis, was decreased by the incubation with dimer A. It was not the case for dimer B. Results indicate that dimer A may inhibit the degradation of DNA during apoptosis. Taken together, it is likely that dimer A exerts both proapoptotic action and inhibitory action on late stage of apoptosis in rat thymocytes.

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“…Almost all dead cells belong to the Asterisks indicate a significant difference (*P Ͻ 0.05, **P Ͻ 0.005) between the control and the group treated with TeCl 2 and between the group treated with DPDT and the group treated simultaneously with DPDT and Z-VAD-FMK, as indicated with arrows. population of shrunken cells, and the membranes of other shrunken cells remain selectively permeable across the membranes only because they are not stained with propidium (Nakao et al, 2003;Umebayashi et al, 2004). Furthermore, cell shrinkage is one of the common morphological changes in cells undergoing apoptosis (Beauvais et al, 1995), and it seems to occur before cytochrome C release from mitochondria, caspase activation, and DNA fragmentation (Wolf et al, 1997;Hughes and Cidlowski, 1998;Maeno et al, 2000).…”

Section: Cell Shrinkage As Parametermentioning

confidence: 99%

“…Shrinkage seems to occur before some characteristic changes in apoptosis such as cytochrome C release from mitochondria, caspase activation, and DNA fragmentation, although controversy remains regarding the relationship between cell shrinkage and ensuing cell death (Wolf et al, 1997;Hughes and Cidlowski, 1998;Maeno et al, 2000). By using the parameter of cell shrinkage, the cytotoxicity test is highly sensitive to cytotoxic chemical compounds (Nakao et al, 2003;Umebayashi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Cytometric analysis of adverse action of diphenyl ditelluride on rat thymocytes: Cell shrinkage as a cytotoxic parameter

Iwase

Tatsuishi

Nishimura

et al. 2004

Environmental Toxicology

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Despite the growing use of organotellurium compounds in the chemical and biomedical fields, there has been no great concern about their toxicity until now. To test the possibility that diphenyl ditelluride (DPDT) and tellurium chloride (TeCl2), organic and inorganic tellurium compounds, may exert adverse action on mammals, their effects on rat thymocytes were examined under in vitro conditions using a flow cytometer with fluorescent probes. Incubation of thymocytes with DPDT at 300 nM or more for 24 h significantly increased the populations of shrunken cells and of cells with hypodiploidal DNA. Z-VAD-FMK, a paninhibitor of caspases, greatly suppressed the DPDT-induced increase in the hypodiploidal cell population, suggesting the involvement of caspase activation in DPDT toxicity. Hence, it is possible that DPDT would increase the population of thymocytes undergoing apoptosis if the blood concentration in mammals reached at least 300 nM or more. TeCl2 was much less potent than DPDT in increasing the population of hypodiploidal cells.

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Tri-n-butyltin-induced cytotoxicity on rat thymocytes in presence and absence of serum

Cited by 19 publications

References 25 publications

The effect of tributyltin on human eosinophylic leukemia EoL-1 cells

The effect of tributyltin on human eosinophylic leukemia EoL-1 cells

Cytotoxic Characteristics of Two Isomeric Dimers Produced by Oxidation of Sesamol, an Antioxidant in Sesame Oil

Cytometric analysis of adverse action of diphenyl ditelluride on rat thymocytes: Cell shrinkage as a cytotoxic parameter

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