TRH regulates action potential shape in cerebral cortex pyramidal neurons

Rodríguez-Molina, Víctor; Patiño, Javier González; Vargas, Yamili; Sánchez-Jaramillo, Edith; Joseph‐Bravo, Patricia; Charlí, Jean-Louis

doi:10.1016/j.brainres.2014.05.015

Cited by 7 publications

(6 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, visible signal was developed with DAB solution. Then, stained sections were mounted, desiccated overnight and processed for ISHH against GAD67 as described before in detail (Rodriguez-Molina et al, 2014). Briefly, sections were hybridized with a 572 bp single stranded [ 35 S]–UTP (Perkin-Elmer NEG 039H) labeled RNA probe, complementary to the rat GAD67 cDNA [nucleotides 1,265–1,836 of NM_017007.1 sequence], donated by Dr. Petersen (Hays et al, 2002).…”

Section: Methodsmentioning

confidence: 99%

Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus

Cabral

Portiansky

Sánchez-Jaramillo

et al. 2016

Psychoneuroendocrinology

Self Cite

View full text Add to dashboard Cite

Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of γ-aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)/NPY neurons. These data support the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore, these data suggest that the neuronal circuits mediating ghrelin’s orexigenic action vs. its role as a stress signal are anatomically dissociated.

show abstract

Section: Methodsmentioning

confidence: 99%

Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus

Cabral

Portiansky

Sánchez-Jaramillo

et al. 2016

Psychoneuroendocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Measurements started with control recording (1 min). Then a single bolus of the dopamine D2 receptor agonist sumanirole (30 μM, Tocris No.2773 Abingdon, UK) (52) or TRH (10 μM, Thermo Fisher [Bachem] No.4002023.0025 MA, USA) (53) was pipetted into the aCSF-filled measurement chamber and recording continued for another 7 min. Each neuron served as its own control when treatment effects were evaluated.…”

Section: Methodsmentioning

confidence: 99%

Development of a versatile LCM-Seq method for spatial transcriptomics of fluorescently-tagged cholinergic neuron populations

Rumpler

Göcz

Skrapits

et al. 2023

Preprint

View full text Add to dashboard Cite

Single-cell transcriptomics are powerful tools to define neuronal cell types based on co-expressed gene clusters. Limited RNA input in these technologies necessarily compromises transcriptome coverage and accuracy of differential expression analysis. We propose that bulk RNA-sequencing of neuronal pools defined by spatial position offers an alternative strategy to overcome these technical limitations. We report an LCM-Seq method which allows deep transcriptome profiling of fluorescently-tagged neuron populations isolated with laser-capture microdissection (LCM) from histological sections of transgenic mice. Mild formaldehyde-fixation of ZsGreen marker protein, LCM sampling of ∼300 pooled neurons, followed by RNA isolation, library preparation and RNA-sequencing with methods optimized for nanogramm amounts of moderately degraded RNA enabled us to detect ∼15,000 different transcripts in fluorescently-labeled cholinergic neuron populations. The versatile LCM-Seq method showed excellent accuracy in quantitative studies, with 2,891 transcripts expressed differentially between the spatially defined and clinically relevant cholinergic neuron populations of the caudate-putamen and medial septum.

show abstract

“…The AP threshold was detected using a custom algorithm described previously by Baufreton et al ( 2005 ). The half-width of AP was measured as a duration between 50% rise time and 50% decay time (measured from baseline) of APs (Rodríguez-Molina et al, 2014 ). The afterhyperpolarization (AHP) peak amplitude was the difference between the AP threshold and the most negative voltage reached during the AHP.…”

Section: Methodsmentioning

confidence: 99%

Estradiol decreases the excitability of RA projection neurons in adult male zebra finches

Zhang

Sun²,

Wu³

et al. 2023

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Zebra finches are essential animal models for studying learned vocal signals. The robust nucleus of the arcopallium (RA) plays an important role in regulating singing behavior. Our previous study showed that castration inhibited the electrophysiological activity of RA projection neurons (PNs) in male zebra finches, demonstrating that testosterone modulates the excitability of RA PNs. Testosterone can be converted into estradiol (E2) in the brain through aromatase; however, the physiological functions of E2 in RA are still unknown. This study aimed to investigate the electrophysiological activities of E2 on the RA PNs of male zebra finches through patch-clamp recording. E2 rapidly decreased the rate of evoked and spontaneous action potentials (APs) of RA PNs, hyperpolarized the resting membrane potential, and decreased the membrane input resistance. Moreover, the G-protein–coupled membrane-bound estrogen receptor (GPER) agonist G1 decreased both the evoked and spontaneous APs of RA PNs. Furthermore, the GPER antagonist G15 had no effect on the evoked and spontaneous APs of RA PNs; E2 and G15 together also had no effect on the evoked and spontaneous APs of RA PNs. These findings suggested that E2 rapidly decreased the excitability of RA PNs and its binding to GPER suppressed the excitability of RA PNs. These pieces of evidence helped us fully understand the principle of E2 signal mediation via its receptors to modulate the excitability of RA PNs in songbirds.

show abstract

TRH regulates action potential shape in cerebral cortex pyramidal neurons

Cited by 7 publications

References 45 publications

Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus

Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus

Development of a versatile LCM-Seq method for spatial transcriptomics of fluorescently-tagged cholinergic neuron populations

Estradiol decreases the excitability of RA projection neurons in adult male zebra finches

Contact Info

Product

Resources

About