TRH and TRH-like peptide expression in rat following episodic or continuous corticosterone

Pekary, A. Eugene; Sattin, Albert; Blood, James; Furst, Stephanie

doi:10.1016/j.psyneuen.2008.06.001

Cited by 12 publications

(7 citation statements)

References 58 publications

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“…Reproductive functions and testosterone release are strongly inhibited by stress as survival is paramount for successful procreation [59]. We have previously shown that, in contrast to all other brain and peripheral tissues studied, testicular TRH-like peptides decline markedly in response to a single high-dose ip injection of corticosterone [42] or following episodic but not continuous administration of this glucocorticoid due to downregulation of the cognate receptors [60]. Serum corticosterone levels are highly correlated with TRH and 5 TRH-like peptide levels in testis, consistent with the pronounced sensitivity of rat testis to stress [61], even the diurnal fluctuations of serum corticosterone in unstressed rats (▶ table 3).…”

Section: Discussionmentioning

confidence: 99%

TRH and TRH-Like Peptide Levels Co-Vary with Reproductive and Metabolic Rhythms

Pekary

Sattin

2016

Horm Metab Res

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Photoperiod-synchronized rhythms in non-CSN tissues persist in total darkness. Clock genes involved in maintaining regular biorhythms within the suprachiasmatic nucleus (SCN) of the hypothalamus are expressed in extra-CNS tissues and continue periodic expression in vitro. Understanding the details of how the SCN clock is coupled with peripheral clocks is only incompletely understood and may involve a multiplicity of feedback systems. The present study is an extension of our previous work showing that brain levels of TRH (pGlu-His-Pro-NH) and TRH-like peptides (X-TRH: pGlu-X-Pro-NH, where "X" can be any amino acid residue) fluctuate throughout the day-night cycle. Male rats were maintained in a stable environment, lights on 6-18 h. TRH and TRH-like peptides in liver, pancreas, testis, prostate, epididymis, and heart were measured at 3, 10, 16, and 22 h. The greatest change in peptide level was a 12-fold increase for TRH in prostate at 16 h relative to the corresponding value at 3 h. The TRH, Tyr-TRH and Phe-TRH levels in liver declined steadily to about 40% of the 3-h values by 22 h. Changes, in the order of decreasing number of significant increases (↑) and/or decreases (↓), were: testis (5↑, 1↓), liver (3↓), epididymis (2↑), prostate (1↑, 1↓) and heart (1↑). Peptide levels in liver and testis correlated with serum leptin and serum corticosterone, respectively, which are potent releasers of these peptides. Testosterone and glucose were also highly correlated. These tripeptides may participate in the regulation of metabolic and reproductive functions, which change during the day-night cycle.

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Section: Discussionmentioning

confidence: 99%

TRH and TRH-Like Peptide Levels Co-Vary with Reproductive and Metabolic Rhythms

Pekary

Sattin

2016

Horm Metab Res

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“…In an effort to identify molecular correlates of riluzole efficacy, a separate group of mice were chronically exposed to CORT using a protocol that mimics the circadian rhythmicity of CORT hyper-secretion in stressed rats (Pekary et al, 2008), induces multiple depressive-like behaviors that persist for a significant duration of the animal’s lifespan (Gourley et al 2008a,b,c; Gourley and Taylor 2009), diminishes hippocampal neurogenesis (David et al 2009), and disrupts hypothalamic-pituitary-adrenal axis negative feedback (Gourley et al 2009). Consistent with neurotrophic hypotheses of depression and antidepressant efficacy (Duman et al 1997), the corticosteroid protocol used here also reduces hippocampal BDNF and phosphorylation of downstream targets including cAMP-response Element Binding Protein (CREB) (fig.4; Gourley et al 2008b).…”

Section: Discussionmentioning

confidence: 99%

Antidepressant-like properties of oral riluzole and utility of incentive disengagement models of depression in mice

et al. 2011

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Rationale The neuroprotective agent riluzole has antidepressant-like properties in humans, but its mechanisms of action are unclear. Despite the increasing utility of transgenic and knockout mice in addressing such issues, previous studies aimed at characterizing biochemical mechanisms have been conducted in rats. Objectives We sought to optimize an oral riluzole administration protocol with antidepressant-like consequences in C57BL/6 mice, a common background strain in genetically-modified mice. Methods Riluzole (6–60 μg/ml) was dissolved in tap water and replaced regular drinking water for up to 3 weeks; sensitivity to tail suspension, forced swimming, and the locomotor response to extinction training in a model of “incentive disengagement” were tested. Peripheral and central effects of long-term 60 μg/ml treatment were also evaluated. Results Riluzole had dose-dependent antidepressant-like effects in the forced swim test and like chronic fluoxetine, exerted antidepressant-like actions in an adaptation of the “incentive disengagement” model at the highest concentration tested. This 60 μg/ml concentration also restored hippocampal Brain-derived Neuroptrophic Factor (BDNF) expression after chronic corticosteroid exposure and increased glutamate glial transporter 1 (GLT-1, or EAAT2) expression without significantly affecting baseline locomotor activity, thymus and adrenal gland weights, or blood serum corticosterone. The lowest 6 μg/ml concentration increased locomotor activity, consistent with an anxiolytic-like effect. Conclusions Riluzole’s therapeutic potential for treating mood disorders may involve GLT-1 and BDNF, and we suggest this protocol could be used to further characterize its precise long-term biochemical mechanisms of action in animal models of depression.

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“…[180, 181] For TRH, Pekary and colleagues did a series of studies on relative TRH and TRH-like peptides level changes in various rat brain region tissue with HPLC-RIA under different drug treatment. [182–184] And SOM levels in the rat nucleus accumbens (NAc) was investigated, showing release evoked by chronic administration of antidepressants. [185]…”

Section: Neuropeptidesmentioning

confidence: 99%

Review of recent advances in analytical techniques for the determination of neurotransmitters

Perry

Kennedy

2009

Analytica Chimica Acta

330

221

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Methods and advances for monitoring neurotransmitters in vivo or for tissue analysis of neurotransmitters over the last five years are reviewed. The review is organized primarily by neurotransmitter type. Transmitter and related compounds may be monitored by either in vivo sampling coupled to analytical methods or implanted sensors. Sampling is primarily performed using microdialysis, but low-flow push-pull perfusion may offer advantages of spatial resolution while minimizing the tissue disruption associated with higher flow rates. Analytical techniques coupled to these sampling methods include liquid chromatography, capillary electrophoresis, enzyme assays, sensors, and mass spectrometry. Methods for the detection of amino acid, monoamine, neuropeptide, acetylcholine, nucleoside, and soluable gas neurotransmitters have been developed and improved upon. Advances in the speed and sensitivity of these methods have enabled improvements in temporal resolution and increased the number of compounds detectable. Similar advances have enabled improved detection at tissue samples, with a substantial emphasis on single cell and other small samples. Sensors provide excellent temporal and spatial resolution for in vivo monitoring. Advances in application to catecholamines, indoleamines, and amino acids have been prominent. Improvements in stability, sensitivity, and selectivity of the sensors have been of paramount interest.

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TRH and TRH-like peptide expression in rat following episodic or continuous corticosterone

Cited by 12 publications

References 58 publications

TRH and TRH-Like Peptide Levels Co-Vary with Reproductive and Metabolic Rhythms

TRH and TRH-Like Peptide Levels Co-Vary with Reproductive and Metabolic Rhythms

Antidepressant-like properties of oral riluzole and utility of incentive disengagement models of depression in mice

Review of recent advances in analytical techniques for the determination of neurotransmitters

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