Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic development during in vitro oocyte maturation

Lucas, Caroline Gomes; Remião, Mariana H.; Komninou, Eliza Rossi; Domingues, William Borges; Haas, Cristina; Leon, Priscila Marques Moura de; Campos, Vinícius Farias; Ourique, Aline Ferreira; Guterres, Sílvia Stanisçuaski; Pohlmann, Adriana Raffin; Basso, Andrea; Seixas, Fabiana Kömmling; Beck, Ruy Carlos Ruver; Collares, Tiago

doi:10.1016/j.reprotox.2015.10.004

Cited by 16 publications

(15 citation statements)

References 51 publications

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“…Many groups worldwide have achieved blastocyst rates similar to the ones reported here (∼40 %), indicating that co-culture with stem cells was as efficient as current IVEP protocols in bovines, most of them using supplemented IVC medium [10,[50][51][52][53][54]. Therefore, further identification of molecules produced by stem cells for embryo development could help to improve IVEP not only in bovine but also in other species such as humans.…”

Section: Discussionsupporting

confidence: 76%

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Miranda

Nascimento

Costa

et al. 2016

J Assist Reprod Genet

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Purpose Despite advances in the composition of defined embryo culture media, co-culture with somatic cells is still used for bovine in vitro embryo production (IVEP) in many laboratories worldwide. Granulosa cells are most often used for this purpose, although recent work suggests that co-culture with stem cells of adult or embryonic origin or their derived biomaterials may improve mouse, cattle, and pig embryo development. Materials and methods In experiment 1, in vitro produced bovine embryos were co-cultured in the presence of two concentrations of bovine adipose tissue-derived mesenchymal cells (b-ATMSCs; 10 3 and 10 4 cells/mL), in b-ATMSC preconditioned medium (SOF-Cond), or SOF alone (control). In experiment 2, co-culture with 10 4 b-ATMSCs/mL was compared to the traditional granulosa cell co-culture system (Gran). Results In experiment 1, co-culture with 10 4 b-ATMSCs/mL improved blastocyst rates in comparison to conditioned and control media (p < 0.05). Despite that it did not show difference with 10 3 b-ATMSCs/mL (p = 0.051), group 10 4 bATMSCs/mL yielded higher results of blastocyst production.In experiment 2, when compared to group Gran, co-culture with 10 4 b-ATMSCs/mL improved not only blastocyst rates but also quality as assessed by increased total cell numbers and mRNA expression levels for POU5F1 and G6PDH (p < 0.05). Conclusions Co-culture of bovine embryos with b-ATMSCs was more beneficial than the traditional co-culture system with granulosa cells. We speculate that the microenvironmental modulatory potential of MSCs, by means of soluble substances and exosome secretions, could be responsible for the positive effects observed. Further experiments must be done to evaluate if this beneficial effect in vitro also translates to an increase in offspring following embryo transfer. Moreover, this study provides an interesting platform to study the basic requirements during preimplantation embryo development, which, in turn, may aid the improvement of embryo culture protocols in bovine and other species.

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Section: Discussionsupporting

confidence: 76%

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Miranda

Nascimento

Costa

et al. 2016

J Assist Reprod Genet

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“…Nevertheless, incorporation of drugs into nanoparticles with a larger size (> 500 ηm) could increase their permanence in certain types of tissues (Tada et al, ) and then increase their time of action. The lipidic nanoparticles containing tretinoin (average size of 250 ηm) (Lucas et al, ) and the polymeric nanoparticles containing melatonin (average size of 197 and 171 ηm) (Komninou et al, ; Remião et al, ) were successfully used on the in vitro maturation of bovine oocytes. Although the size of the nanoparticles obtained in this study was considered large, the NP20 treatment had more metabolically active mitochondria (an indicative of mitochondrial function (Brand & Nicholls, )) than the control group (MEM), which was probably due to the relation between larger size and longer time of action.…”

Section: Discussionmentioning

confidence: 99%

“…An innovative way of improving drug solubility and chemical stability, and reducing its toxicity on the in vitro culture is the use of polymeric carrier nanoparticles for substance transportation. Some studies have reported that such nanoparticles containing the antioxidant compounds tretinoin (Lucas et al, ) or melatonin (Komninou et al, ; Remião et al, ) improved bovine embryo quality after in vitro culture. Among synthetic polymers used in the production of nanoparticles, polylactic‐co‐glycolic acid (PLGA) stands out due to its excellent biocompatibility, low toxicity and high capacity to encapsulate drugs (Essa, Rabanel, & Hildgen, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

Nascimento

Silva

Alves

et al. 2018

Reprod Domestic Animals

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Contents This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in the culture medium of isolated ovine preantral follicles. HERP was characterized by high‐performance liquid chromatography (HPLC) and determination of flavonoid content, and the nanoparticles by the mean particle diameter, polydispersity index (PI) and encapsulation efficiency (EE). The effect of HERP (10 and 20 ηg/ml—HERP10 and HERP20 groups) and nanoparticles (NP10 and NP20 groups) on isolated secondary follicles cultured in vitro for 12 days was observed by morphological evaluation, oxidative stress markers (reactive oxygen species—ROS and glutathione—GSH) and active mitochondria. HPLC showed formononetin as the major compound in the HERP (63.92 ± 0.21 μg/mL). The content of flavonoids ranged from 2.14% to 11.00%. The nanoparticles showed mean diameter of 287.5 ± 3.9 and 479 ± 18.1 ηm; PI of 0.117 ± 0.018 and 0.316 ± 0.039; and EE of 67.15% and 41%, respectively, for the NP10 and NP20 groups. After 12 days of culture, HERP20 and NP20 increased (p < 0.05) the percentage of normal follicles compared to NP10. HERP20 showed significantly higher percentages of antrum formation than control medium (MEM) and NP10 (p < 0.05). HERP20 also showed an increase (p < 0.05) in mitochondrial activity compared to the other treatments, except NP20 (p > 0.05), and increased GSH levels (p < 0.05) compared to MEM and HERP10. In conclusion, use of HERP (20 ηg/ml) on in vitro culture of isolated ovine preantral follicles can increase antrum formation, mitochondrial activity and GSH levels.

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“…However, a growing number of studies have applied nanotechnology in reproductive biology to target and sort specific populations of gametes and to optimize gene transfer and editing methodologies (Barkalina, Charalambous, Jones, & Coward, ; Remião et al, ). In addition, nanoparticulated systems have shown great potential as carriers to deliver molecules into gametes and embryos to improve their developmental competence in vitro (Barkalina, Charalambous, et al, ; Barkalina et al, ; Komninou et al, ; Lucas et al, ; Remião et al, ).…”

Section: Nanotechnologymentioning

confidence: 99%

“…IVM medium supplementation with tretinoin (TTN)‐loaded lipid‐core nanocapsules (TTN‐LNC) decreased ROS production and increased the percentage of embryos that developed to the blastocyst stage when compared to nonencapsulated TTN. In addition, nanoencapsulation allowed for the use of lower concentrations of TTN, minimizing the risk of cytotoxic effects related with its use (Lucas et al, ). Similarly, melatonin‐loaded lipid‐core nanocapsules (Mel‐LNC) added during bovine IVM and IVC reduced ROS levels and apoptosis in embryos, increased the cleavage and blastocyst rates, upregulated antioxidant genes, and downregulated proapoptotic genes when compared to nonencapsulated melatonin (Mel) (Komninou et al, ; Remião et al, ).…”

Section: Nanotechnologymentioning

confidence: 99%

Applications of omics and nanotechnology to improve pig embryo production in vitro

Lucas

Chen

Seixas

et al. 2019

Molecular Reproduction Devel

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An appropriate environment to optimize porcine preimplantation embryo production in vitro is required as genetically modified pigs have become indispensable for biomedical research and agriculture. To provide suitable culture conditions, omics technologies have been applied to elucidate which metabolic substrates and pathways are involved during early developmental processes. Metabolomic profiling and transcriptional analysis comparing in vivo‐ and in vitro‐derived embryos have demonstrated the important role of amino acids during preimplantation development. Transcriptional profiling studies have been helpful in assessing epigenetic reprogramming agents to allow for the correction of gene expression during the cloning process. Along with this, nanotechnology, which is a highly promising field, has allowed for the use of engineered nanoplatforms in reproductive biology. A growing number of studies have explored the use of nanoengineered materials for sorting, labeling, and targeting purposes; which demonstrates their potential to become one of the solutions for precise delivery of molecules into gametes and embryos. Considering the contributions of omics and the recent progress in nanoscience, in this review, we focused on their emerging applications for current in vitro pig embryo production systems to optimize the generation of genetically modified animals.

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Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic development during in vitro oocyte maturation

Cited by 16 publications

References 51 publications

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

Applications of omics and nanotechnology to improve pig embryo production in vitro

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