Treponemicidal Antibody Measured by the “Washed-Killing” Assay Correlates With Immunity in Experimental Rabbit Syphilis

Lewinski, Michael A.; Miller, James N.; Champion, Cheryl I.; Walker, Emma C; Borenstein, L A; Gayek, Richard J.; Lovett, Michael; Blanco, David R.

doi:10.1097/00007435-199501000-00006

Cited by 5 publications

(7 citation statements)

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“…Protection against sexual transmission of most of these pathogens has been associated with local production of specific antibodies (6,19,20,23,30,31,33,40,45,46). Both immunoglobulin G (IgG) and secretory IgA appear to be important.…”

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confidence: 99%

Specific-Antibody-Secreting Cells in the Rectums and Genital Tracts of Nonhuman Primates following Vaccination

Eriksson¹,

Quiding‐Järbrink²,

Osek³

et al. 1998

Infect Immun

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To determine optimal strategies to induce specific-antibody-secreting cells (specific ASC) in the rectal and vaginal mucosae, we immunized monkeys with a prototype mucosal immunogen, cholera toxin (CT), given locally or via gastric or parenteral administration. Repeated rectal or vaginal CT immunizations induced strong mucosal and systemic ASC responses. The mucosal responses were, however, confined to the immunization sites and comprised high levels of both specific antitoxin immunoglobulin A (IgA) and IgG. Large numbers of specific IgA and IgG ASC were detected in cell suspensions from dissociated genital and rectal tissues, demonstrating local accumulation of effector B cells at these sites. Intragastric immunization with CT did not per se give rise to cervicovaginal or rectal ASC responses but did prime for a rectal IgA ASC response to local booster immunization. Both rectal and vaginal immunizations also induced circulating blood IgG ASC and IgA ASC. In conclusion, these results show that local administration of antigen to the rectal or vaginal mucosa results in higher ASC responses than systemic or distant mucosal delivery. Furthermore, both the vaginal and the rectal mucosae can serve as inductive sites for systemic ASC responses. These observations should be relevant to the development of vaccines against sexually transmitted diseases such as that caused by human immunodeficiency virus.

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confidence: 99%

Specific-Antibody-Secreting Cells in the Rectums and Genital Tracts of Nonhuman Primates following Vaccination

Eriksson¹,

Quiding‐Järbrink²,

Osek³

et al. 1998

Infect Immun

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“…However, complete protection of treponemes from treponemicidal antibodies by cardiolipin antibodies did not occur. According to Lewinski et al [23] the TPI test proceeds in a 'two hit' mechanism. In the first step, antibodies directed against the O W antigens activate the complement membrane attack complex C5b-9 and the latter perforates the outer membrane.…”

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confidence: 99%

Influence of cardiolipin antibodies on the binding of treponemal specific antibodies in the fluorescence treponemal antibody absorption test and the Treponema pallidum immobilisation test

Borkhardt

Zielinski²

1997

Journal of Medical Microbiology

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The aim of the present study was to investigate the biological role of cardiolipin antibodies during Treponema pallidum infection. Inhibition of the binding of treponemal specific antibodies at the early and late stages of infection by cardiolipin antibodies was shown in the fluorescence treponemal antibody absorption (FTA-ABS) test and T.pallidurn immobilisation (TPI) test. Incubation of treponemes with cardiolipin antibodies followed by a second incubation with treponemal specific antibodies resulted in a reduction of the titres of the FTA-ABS test and the TPI test. The findings suggest that cardiolipin antibody production should be considered as a virulence mechanism of pathogenic treponemes with the purpose of evading the host defence mechanisms.

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“…T. paraluiscuniculi is sensitive to some antibiotics, similar to T. palidum, the pathogenic organism in human syphilis [5,6,10,15,16,19]. Penicillin (PC) is commonly used to treat human syphilis [2,3,7], except in PC allergic individuals [1], but antibiotic use is more strictly limited in rabbits, due to frequent adverse effects [8,9,12,14,20].…”

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confidence: 99%

Chloramphenicol Treatment for Rabbit Syphilis

Saito

Hasegawa

2004

The Journal of Veterinary Medical Science

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ABSTRACT. Penicillin, the recommended treatment for rabbit syphilis, sometimes induces adverse effects. The efficacy of oral chloramphenicol was evaluated in 39 cases of rabbit syphilis to establish a safe and efficient treatment for this disease in companion rabbits. All cases clinically improved and recovered promptly. Fourteen of 39 cases (35.9%) relapsed, but most remained chloramphenicol sens itive. Since safety take priority over efficacy in treating syphilis in companion rabbits, chloramphenicol should be chosen as a first-line agent, as a general rule. Three-week administration of chloramphenicol may be adequate at the initial onset of disease. When relapse occurs repeatedly or the rabbit owner cannot administer the medicine adequately, treatment with penicillin should be considered. KEY WORDS: antibiotic, rabbit syphilis, relapse.

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Treponemicidal Antibody Measured by the “Washed-Killing” Assay Correlates With Immunity in Experimental Rabbit Syphilis

Cited by 5 publications

References 0 publications

Specific-Antibody-Secreting Cells in the Rectums and Genital Tracts of Nonhuman Primates following Vaccination

Specific-Antibody-Secreting Cells in the Rectums and Genital Tracts of Nonhuman Primates following Vaccination

Influence of cardiolipin antibodies on the binding of treponemal specific antibodies in the fluorescence treponemal antibody absorption test and the Treponema pallidum immobilisation test

Chloramphenicol Treatment for Rabbit Syphilis

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