Trennung einiger Thyreostatica durch Hochdruck-Flüssigkeits-Chromatographie

Wildanger, W.

doi:10.1007/bf02279281

Cited by 13 publications

(5 citation statements)

References 2 publications

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“…Detection limits in the low mg/L range are comparable or better than those obtained in the chromatographic assays. The advantages of this CE method over HPLC [5][6][7] are the following: it is rapid; it does not require solvent gradient or column equilibration, and no organic solvents are needed for elution purposes. Compared with GC methods [8][9][10], this approach is simpler, more rapid, and inexpensive.…”

Section: Discussionmentioning

confidence: 99%

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An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection

et al. 2005

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This paper reports the development of a method based on capillary electrophoresis with laser-induced fluorescence detection for the simultaneous determination of thiouracil (TU) and phenylthiouracil (PhTU) with high sensitivity (nanomolar range, i.e., attomoles detected). After derivatization with 5-iodoacetamidofluorescein, the analytes were separated by capillary zone electrophoresis using 20 mM phosphate buffer (pH 10.0) and quantified by fluorescence detection. The linearity range, precision, recovery, and detection limits were determined, and the method was shown to be applicable for the determination of TU and PhTU in spiked feed samples and urine.

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Section: Discussionmentioning

confidence: 99%

“…and animal feeds; these include TLC [4], HPLC with UV and/or electrochemical detection [5][6][7], GC with electron-capture nitrogen-phosphorus, or mass spectrometric detection [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection

et al. 2005

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“…Only Buick et al (1998) was able to reach a lower LOD, in the range of 10 g kg −1 [65]. As for the detection UV (ultraviolet) [85][86][87][88][89][90], electrochemical [88,89], chemiluminescence [91] and diode array detection (DAD) [65] were reported. With the introduction of atmospheric pressure interfaces like ESI (electrospray ionization) and APCI (atmospheric pressure chemical ionization), liquid chromatography coupled to mass spectrometry (LC-MS) became the method of choice for (thyreostatic) residue control [52,61,64].…”

Section: Detectionmentioning

confidence: 99%

Analysis of thyreostats: A history of 35 years

Bussche

Noppe

Verheyden

et al. 2009

Analytica Chimica Acta

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“…A lot of methods have been developed for the determination of thyreostatics residue in samples, such as highperformance thin layer chromatography [7], HPLC with ultraviolet (UV) or electrochemical or MS detection [8][9][10], GC with electron-capture or nitrogen-phosphorus or MS detection [11][12][13][14], our recently developed flow-injection analysis equipped with electrochemiluminescent detector [15], electrochemical analysis [16][17][18][19][20] and electrophoresis [21][22][23]. Although GC and HPLC are powerful analytical methods, they suffer from relatively high instrumental cost, use of too much samples and solvents, which may increase environmental burden, and too much time consumption.…”

Section: Introductionmentioning

confidence: 99%

Determination of thyreostatics in animal feeds by CE with electrochemical detector

et al. 2009

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A simple, rapid, reproducible and sensitive method based on CE with electrochemical detector was developed for the simultaneous determination of five thyreostatics including 2-thiouracil (TU), 6-methyl-2-thiouracil (MTU), 6-propyl-2-thiouracil (PTU), 6-phenyl-2-thiouracil (PhTU) and methimazole (TAP) in animal feeds. A home-made wall-jet electrochemical detector with a 300 microm diameter platinum-disk-working electrode was equipped at the end of separation capillary and used to detect oxidation currents of these thyreostatics. Under the optimum experimental conditions, TU, MTU, PTU, PhTU and TAP could be well separated within 15 min at the separation voltage of 16 kV in 20 mmol/L sodium borate buffer (pH 9.2). The detection limits (S/N=3) of the five thyreostatics in animal feeds were found to be 7.6 microg/kg for TAP, 25 microg/kg for PTU, 15 microg/kg for PhTU, 18 microg/kg for TU and 20 microg/kg for MTU by the developed CE with electrochemical detector method coupled with solid-phase extraction sample pretreatment technique.

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Trennung einiger Thyreostatica durch Hochdruck-Flüssigkeits-Chromatographie

Cited by 13 publications

References 2 publications

An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection

An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection

Analysis of thyreostats: A history of 35 years

Determination of thyreostatics in animal feeds by CE with electrochemical detector

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