Trends on (elemental and molecular) mass spectrometry based strategies for speciation and metallomics

Montes-Bayón, Marı́a; Sharar, Mona; Corte‐Rodríguez, Mario

doi:10.1016/j.trac.2017.09.025

Cited by 33 publications

(29 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 103 However, it is important to understand that these preservatives can affect some chemical properties of the interest species, changing their oxidation states. 104 Centrifugation and filtration are also common steps for removing materials such as cellular components. 96 In some cases, vacuum devices are needed to increase speed.…”

Section: State Of the Art In Metallomics: From Sampling To Quantificamentioning

confidence: 99%

“…LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry), 27 , 28 SIMS (secondary ion mass spectrometry), 29 , 30 X-ray fluorescence spectrometry (XRF), 31 , 32 and LIBS (laser-induced breakdown spectroscopy) 33 , 34 are also important techniques to obtain information on the spatial distribution of metals in solid samples, such as tissues and cells. 17 , 19 , 35 , 36 …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Understanding the relationship between viral infections and trace elements from a metallomics perspective: implications for COVID-19

Jesus

Andrade

2020

Metallomics

View full text Add to dashboard Cite

show abstract

Section: State Of the Art In Metallomics: From Sampling To Quantificamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Understanding the relationship between viral infections and trace elements from a metallomics perspective: implications for COVID-19

Jesus

Andrade

2020

Metallomics

View full text Add to dashboard Cite

show abstract

“…It provides data for the elucidation of the mechanism of antitumor therapy, the behaviour of the intact drug, its biotransformation in clinical samples at physiologically relevant concentrations and, as the final goal, its dosage adjustment. This information can be largely acquired by a speciation analysis [22][23][24][25][26][27]. For a metalloprotein speciation analysis, the most frequently applied was high-performance liquid chromatography (HPLC) hyphenated to inductively coupled plasma mass spectrometry (ICP-MS) detector.…”

Section: Introductionmentioning

confidence: 99%

Binding Kinetics of Ruthenium Pyrithione Chemotherapeutic Candidates to Human Serum Proteins Studied by HPLC-ICP-MS

et al. 2020

View full text Add to dashboard Cite

The development of ruthenium-based complexes for cancer treatment requires a variety of pharmacological studies, one of them being a drug’s binding kinetics to serum proteins. In this work, speciation analysis was used to study kinetics of ruthenium-based drug candidates with human serum proteins. Two ruthenium (Ru) complexes, namely [(η6-p-cymene)Ru(1-hydroxypyridine-2(1H)-thionato)Cl] (1) and [(η6-p-cymene)Ru(1-hydroxypyridine-2(1H)-thionato)pta]PF6 (2) (where pta = 1,3,5-triaza-7-phosphaadamantane), were selected. Before a kinetics study, their stability in relevant media was confirmed by nuclear magnetic resonance (NMR). Conjoint liquid chromatography (CLC) monolithic column, assembling convective interaction media (CIM) protein G and diethylamino (DEAE) disks, was used for separation of unbound Ru species from those bound to human serum transferrin (Tf), albumin (HSA) and immunoglobulins G (IgG). Eluted proteins were monitored by UV spectrometry (278 nm), while Ru species were quantified by post-column isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS). Binding kinetics of chlorido (1) and pta complex (2) to serum proteins was followed from 5 min up to 48 h after incubation with human serum. Both Ru complexes interacted mainly with HSA. Complex (1) exhibited faster and more extensive interaction with HSA than complex (2). The equilibrium concentration for complex (1) was obtained 6 h after incubation, when about 70% of compound was bound to HSA, 5% was associated with IgG, whereas 25% remained unbound. In contrast, the rate of interaction of complex (2) with HSA was much slower and less extensive and the equilibrium concentration was obtained 24 h after incubation, when about 50% of complex (2) was bound to HSA and 50% remained unbound.

show abstract

“…The researchers in this field generally use combination of high-sensitivity atomic detectors, such as inductively coupled plasma mass spectrometry (ICP-MS), different techniques of high performance liquid chromatography (HPLC), and various types of mass spectrometry (MS), to enable metal/nonmetal analyses within the cells, as well as characterization and possible identification of specific metal-binding biomolecules [19][20][21][22][23][24] . Association of multidimensional separation approaches (e.g.…”

Section: Introductionmentioning

confidence: 99%

Characterization and identification of selected metal-binding biomolecules from hepatic and gill cytosols of Vardar chub (Squalius vardarensis Karaman, 1928) using various techniques of liquid chromatography and mass spectrometry

et al. 2019

View full text Add to dashboard Cite

show abstract

Trends on (elemental and molecular) mass spectrometry based strategies for speciation and metallomics

Cited by 33 publications

References 53 publications

Understanding the relationship between viral infections and trace elements from a metallomics perspective: implications for COVID-19

Understanding the relationship between viral infections and trace elements from a metallomics perspective: implications for COVID-19

Binding Kinetics of Ruthenium Pyrithione Chemotherapeutic Candidates to Human Serum Proteins Studied by HPLC-ICP-MS

Characterization and identification of selected metal-binding biomolecules from hepatic and gill cytosols of Vardar chub (Squalius vardarensis Karaman, 1928) using various techniques of liquid chromatography and mass spectrometry

Contact Info

Product

Resources

About