Trends in the biomanufacture of polyhydroxyalkanoates with focus on downstream processing

Kosseva, Maria R.; Rusbandi, Edy

doi:10.1016/j.ijbiomac.2017.09.054

Cited by 124 publications

(58 citation statements)

References 123 publications

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“…The recovery and purification of PHAs are known to contribute significantly to the costs of the polymer. The roles of solvents in extracting PHAs granules from the biomass are to change the permeability of cell membrane and to dissolve the polymer inside the cells . Various studies have been performed to improve the process .…”

Section: Discussionmentioning

confidence: 99%

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

2018

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Polyhydroxyalkanoates (PHAs) has been paid great attention because of its useful thermoplastic properties and complete degradation in various natural environments. But, at industrial level, the successful commercialization of PHAs is limited by the high production cost due to the expensive carbon source and recovery processes. Pseudomonas mendocina PSU cultured for 72 h in mineral salts medium (MSM) containing 2% (v/v) biodiesel liquid waste (BLW) produced 79.7 wt% poly(3-hydroxybutyrate) (PHB) at 72 h. In addition, this strain produced 43.6 wt% poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with 8.6 HV mol% at 60 h when added with 0.3% sodium propionate. The synthesized intracellular PHA granules were recovered and purified by the recently reported biological method using mealworms. The weight average molecular weight (M ) and number average molecular weight (M ) of the biologically extracted PHA were higher than that from the chloroform extraction with comparable melting temperature (T ) and high purity. This study has successfully established a low-cost process to synthesize PHAs from BLW and subsequently confirmed the ability of mealworms to extract PHAs from various kinds of bacterial cells.

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Section: Discussionmentioning

confidence: 99%

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

2018

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“…After biosynthesis, the development of sustainable and inexpensive technologies to recover intracellular PHA from microbial biomass is the final crucial step in the entire PHA production chain. Several comprehensive reviews summarize reported methods for PHA recovery; in principle, such methods resort to the use of practicable PHA-solvents to extract PHA from the cells, the chemical or enzymatic digestion of the microbial biomass accommodating PHA granules, or the mechanical disintegration of the non-PHA cell mass (107)(108)(109). Importantly, well-established methods for PHA extraction, which result in best recovery yields and highest product purity, resort to toxic halogenated solvents, predominately chloroform, which should not take part in a sustainable production chain.…”

Section: Advanced Downstream Processing For Sustainable and Efficientmentioning

confidence: 99%

Switching from petro-plastics to microbial polyhydroxyalkanoates (PHA): the biotechnological escape route of choice out of the plastic predicament?

Koller

2019

The EuroBiotech Journal

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The benefit of biodegradable “green plastics” over established synthetic plastics from petro-chemistry, namely their complete degradation and safe disposal, makes them attractive for use in various fields, including agriculture, food packaging, and the biomedical and pharmaceutical sector. In this context, microbial polyhydroxyalkanoates (PHA) are auspicious biodegradable plastic-like polyesters that are considered to exert less environmental burden if compared to polymers derived from fossil resources. The question of environmental and economic superiority of bio-plastics has inspired innumerable scientists during the last decades. As a matter of fact, bio-plastics like PHA have inherent economic drawbacks compared to plastics from fossil resources; they typically have higher raw material costs, and the processes are of lower productivity and are often still in the infancy of their technical development. This explains that it is no trivial task to get down the advantage of fossil-based competitors on the plastic market. Therefore, the market success of biopolymers like PHA requires R&D progress at all stages of the production chain in order to compensate for this disadvantage, especially as long as fossil resources are still available at an ecologically unjustifiable price as it does today. Ecological performance is, although a logical argument for biopolymers in general, not sufficient to make industry and the society switch from established plastics to bio-alternatives. On the one hand, the review highlights that there’s indeed an urgent necessity to switch to such alternatives; on the other hand, it demonstrates the individual stages of the production chain, which need to be addressed to make PHA competitive in economic, environmental, ethical, and performance-related terms. In addition, it is demonstrated how new, smart PHA-based materials can be designed, which meet the customer’s expectations when applied, e.g., in the biomedical or food packaging sector.

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“…In this context, PHA extraction by solvent-based techniques, and various methods for chemical, enzymatic, and mechanical disintegration of the non-PHA ("residual") biomass are comprehensively summarized in the current literature. However, all these methods have certain disadvantages either from an economic point of view, for ecological considerations, safety aspects, low PHA recovery yield, product pureness, or because of scaling difficulties, which are industrially critical indeed (25)(26)(27). Recent developments to optimize PHA recovery involve the application of harmless solvents (28), supercritical fluids (29), or ionic liquids (30).…”

Section: Polyhydroxyalkanoates (Pha) -A Biological Alternativementioning

confidence: 99%

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

Koller

Braunegg

2018

The EuroBiotech Journal

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Polyhydroxyalkanoates (PHA), the only group of "bioplastics" sensu stricto, are accumulated by various prokaryotes as intracellular "carbonosomes". When exposed to exogenous stress or starvation, presence of these microbial polyoxoesters of hydroxyalkanoates assists microbes to survive."Bioplastics" such as PHA must be competitive with petrochemically manufactured plastics both in terms of material quality and manufacturing economics. Cost-effectiveness calculations clearly show that PHA production costs, in addition to bioreactor equipment and downstream technology, are mainly due to raw material costs. The reason for this is PHA production on an industrial scale currently relying on expensive, nutritionally relevant "1 st -generation feedstocks", such as like glucose, starch or edible oils. As a way out, carbon-rich industrial waste streams ("2 nd -generation feedstocks") can be used that are not in competition with the supply of food; this strategy not only reduces PHA production costs, but can also make a significant contribution to safeguarding food supplies in various disadvantaged parts of the world. This approach increases the economics of PHA production, improves the sustainability of the entire lifecycle of these materials, and makes them unassailable from an ethical perspective.In this context, our EU-funded projects ANIMPOL and WHEYPOL, carried out by collaborative consortia of academic and industrial partners, successfully developed PHA production processes, which resort to waste streams amply available in Europe. As real 2 nd -generation feedstocks", waste lipids and crude glycerol from animal-processing and biodiesel industry, and surplus whey from dairy and cheese making industry were used in these processes. Cost estimations made by our project partners determine PHA production prices below 3 € (WHEYPOL) and even less than 2 € (ANIMPOL), respectively, per kg; these values already reach the benchmark of economic feasibility.The presented studies clearly show that the use of selected high-carbon waste streams of (agro)industrial origin contributes significantly to the cost-effectiveness and sustainability of PHA biopolyester production on an industrial scale.

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Trends in the biomanufacture of polyhydroxyalkanoates with focus on downstream processing

Cited by 124 publications

References 123 publications

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

Switching from petro-plastics to microbial polyhydroxyalkanoates (PHA): the biotechnological escape route of choice out of the plastic predicament?

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

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