Trends in rabbit insemination extenders for fresh and frozen semen. A review

Viudes-de-Castro, M.P.; Vicente, J.S.

doi:10.4995/wrs.2023.18505

Cited by 4 publications

(3 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies have explored ways to enhance the cryopreservation of rabbit sperm by addressing aspects such as freezing techniques and the types and concentrations of cryoprotectants [ 1 , 2 , 3 ]. Although significant technical improvements have been made to the protocols used on rabbit semen in recent years [ 3 , 4 ], the absence of a well-established standard freezing procedure in this species is due to the inconsistent and varied outcomes observed in different studies. Currently, post-thaw results show variable sperm survival rates, averaging between 10% and 60% for total motility and viability and 5% to 35% for progressive motility.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cryopreserving Rabbit Semen: Impact of Varying Sperm Concentrations on Quality and the Standardization of Protocol

Di Iorio,

Lauriola,

Rusco

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

This study aimed to investigate the impact of sperm concentrations on the in vitro quality of cryopreserved rabbit semen. The semen pools (n = 8, from 80 donors) were split into five aliquots with final sperm concentrations of 15, 25, 35, 55, and 75 × 106 per straw. The sperm motility parameters (CASA system) and membrane integrity (flow cytometric analysis) were both evaluated at various stages of the cryopreservation process: fresh semen dilution, cooling, equilibration, and immediately after and 30 min post-thawing. The results indicated the significant influence of the sperm concentration on the total motility (TM) and progressive motility (PM), with a consistent decline in all sperm variables over the time points. Notably, the semen with a final concentration of 15 × 106 exhibited a higher TM and PM after cooling and equilibration. The post-thawing quality (TM, PM) was higher (p < 0.05) in the mid-range sperm concentrations of 25 × 106 (49.9% and 19.7%) and 35 × 106 (46.2% and 19.7%) compared to the other concentrations. This study demonstrated that the sperm concentration per straw played a significant role in specific phases of the cryopreservation process. These findings contribute valuable insights for refining and standardizing the cryopreservation protocol for rabbit semen, emphasizing the importance of the sperm concentration.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Currently, post-thaw results show variable sperm survival rates, averaging between 10% and 60% for total motility and viability and 5% to 35% for progressive motility. These fluctuations emphasize the urgent requirement to enhance and standardize the cryopreservation protocols for rabbit semen [ 1 , 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Cryopreserving Rabbit Semen: Impact of Varying Sperm Concentrations on Quality and the Standardization of Protocol

Di Iorio,

Lauriola,

Rusco

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…The commercial use of artificial insemination in rabbit farming is relatively recent, and it has motivated the introduction of new reproductive rhythms in rabbit production [27,28]. Comparative studies of the productivity of different reproductive rhythms have shown that the reproductive lifespan of the females is negatively affected and litter viability decreases when using intensive reproductive management (mating 4 d post-partum) compared to semi-intensive and semi-extensive management (mating 11 d and 18 d post-partum, respectively) [29,30].…”

Section: Introductionmentioning

confidence: 99%

Changes in Body Reserves, Non-Esterified Fatty Acids, and Leptin during the Reproductive Lifespan of the Rabbit Female

Peiró,

Argente,

García

2023

Animals

View full text Add to dashboard Cite

The aim of this work is to study changes in body weight, perirenal fat thickness (PFT), and non-esterified fatty acid (NEFA) and leptin concentrations throughout the reproductive life of the rabbit female and their correlations when a semi-intensive reproductive rhythm is applied. A total of 46 lactating females were used. Body weight, PFT, and NEFA and leptin concentration were recorded at 12 weeks of age, at first mating and delivery, and at second, third, and fourth mating, 12th d of gestation, and delivery. The highest body weight was detected on the 12th d of any gestation, around 4280 g, and the lowest weight was at delivery, around 4030 g. PFT increased until third mating. NEFA and leptin concentration showed a cyclical pattern throughout the reproductive lifespan of the females. NEFAs presented the highest concentration at delivery within each reproductive cycle and levels decreased over the course of the deliveries (0.423 mmol/L at first delivery, 0.406 mmol/L at second delivery, 0.371 mmol/L at third delivery, and 0.309 mmol/L at fourth delivery). Similar NEFA concentrations at mating and on the 12th d of gestation were obtained. Leptin showed the highest concentrations at mating within each reproductive cycle. Leptin decreased between mating and delivery in all reproductive cycles and it was close to 1 ng/mL HE. Low or null correlations were shown between body weight, PFT, and NEFA and leptin concentration at mating, 12th d of gestation, and delivery. In conclusion, females are able to maintain a semi-intensive reproductive rhythm across four parities weighing around 4 kg from first mating. Females had an increased perirenal fat thickness until third delivery, and their NEFA concentration was maximum at delivery and leptin concentration was maximum at mating. Body weight, PFT, and NEFA and leptin concentration should be measured during critical moments of reproductive life in order to determine body condition and energy mobilization, due to their low or null correlations.

show abstract

Influence of nano‐formulations of clove bud (Syzygium aromaticum) extract on freezing ability, antioxidant capacity, caspase‐3 activity, acrosome reaction and fertility of frozen rabbit semen

Khalil,

Atta,

El‐Ratel

et al. 2023

Reprod Domestic Animals

View full text Add to dashboard Cite

The current study aimed to investigate the impact of nano‐formulations of clove bud ethanolic extract (CBENF) in the extender on sperm characteristics, antioxidant capacity, oxidative biomarkers, enzymatic activity, apoptosis and fertility of post‐thawed rabbit semen. Twelve mature male rabbits semen samples were pooled and cryopreserved in a Tris‐egg yolk‐based extender containing varying concentrations of CBENF (0, 25, 50, 75 and 100 μg/mL). After the equilibration and freezing–thawing process, CBENF (100 μg /mL) significantly enhanced progressive motility, viability and membrane integrity. Conversely, sperm abnormality was significantly reduced by CBENF supplementation. Total antioxidant capacity was increased in the post‐thawed sperm medium, while nitric oxide and malondialdehyde were decreased in all CBENF concentrations. The lactic dehydrogenase and caspase‐3 activities were decreased, whereas the number of live spermatozoa with an intact acrosome was increased in all CBENF concentrations. Conception rate and litter size per doe were higher in doe rabbits inseminated with semen supplemented with 100 μg CBENF/mL than un‐supplemented group (76% vs. 52% and 8.4 vs. 7.7/doe), with no statistical differences. These findings suggest that supplementing rabbit extenders with 100 μg of CBENF/mL could be an effective strategy for enhancing freeze–thawing rabbit sperm attributes and fertility.

show abstract

Trends in rabbit insemination extenders for fresh and frozen semen. A review

Cited by 4 publications

References 78 publications

Cryopreserving Rabbit Semen: Impact of Varying Sperm Concentrations on Quality and the Standardization of Protocol

Cryopreserving Rabbit Semen: Impact of Varying Sperm Concentrations on Quality and the Standardization of Protocol

Changes in Body Reserves, Non-Esterified Fatty Acids, and Leptin during the Reproductive Lifespan of the Rabbit Female

Influence of nano‐formulations of clove bud (Syzygium aromaticum) extract on freezing ability, antioxidant capacity, caspase‐3 activity, acrosome reaction and fertility of frozen rabbit semen

Contact Info

Product

Resources

About