Trends in Population Dynamics of<i>Escherichia coli</i>Sequence Type 131, Calgary, Alberta, Canada, 2006–20161

Peirano, Gisele; Lynch, Tarah; Matsumara, Yasufumi; Nobrega, Diego; Finn, Thomas J.; DeVinney, Rebekah; Pitout, Johann

doi:10.3201/eid2612.201221

Cited by 29 publications

(25 citation statements)

References 36 publications

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“…Additionally, we detected QRDR (Quinolone Resistance Determining Regions) mutations in gyrA (S83L and D87N) and parC (S80I) in high-level quinolone resistant isolates (28), and 14 of these coharbored oqxAB ( Figure 1 ). This was consistent with a previous study where blood isolates of bla CTX-M-27 -producing ST131 E. coli possessed QRDR mutations that enabled high level resistance to quinolones [ 22 ]. Similarly, bla CTX-M-14 , bla CTX-M-55 , bla CTX-M-15 and bla CTX-M-9 -producing E. coli possessed QRDR mutations and carried PMQR genes that enabled high level resistance to quinolones [ 23 ].…”

Section: Resultssupporting

confidence: 93%

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

et al. 2021

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The aim of this study was to elucidate the prevalence of blaCTX-M-27-producing Escherichia coli and transmission mechanisms of blaCTX-M-27 from swine farms in China. A total of 333 E. coli isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive E. coli were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried blaCTX-M-27, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried blaCTX-M-27 genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The blaCTX-M-27 genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with blaCTX-M-27-plasmids indicated that the tra-trb region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing blaCTX-M-27 in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the blaCTX-M-27 gene in E. coli. This study indicated that the predominant vehicle for blaCTX-M-27 transmission has diversified over time and that control strategies to limit blaCTX-M-27 transmission in farm animals are necessary.

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Section: Resultssupporting

confidence: 93%

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

et al. 2021

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“… 44 In the CTX-M-9 group, CTX-M-9 and CTX-M-14 were the most common enzymes, but more recently CTX-M-27 has often been reported. 49–53 CTX-M-2, CTX-M-8, and CTX-M-25 are the most frequent variants within their own groups. The analysis of the upstream sequences flanking the genes encoding CTX-M-2 groups belonged to Kluyvera spp.…”

Section: Esbl Familiesmentioning

confidence: 98%

Extended-spectrum β-lactamases: an update on their characteristics, epidemiology and detection

Castanheira

Simner

Bradford³

2021

JAC-Antimicrobial Resistance

317

309

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Extended-spectrum β-lactamase (ESBL)-producing Gram-negative pathogens are a major cause of resistance to expanded-spectrum β-lactam antibiotics. Since their discovery in the early 1980s, they have spread worldwide and an are now endemic in Enterobacterales isolated from both hospital-associated and community-acquired infections. As a result, they are a global public health concern. In the past, TEM- and SHV-type ESBLs were the predominant families of ESBLs. Today CTX-M-type enzymes are the most commonly found ESBL type with the CTX-M-15 variant dominating worldwide, followed in prevalence by CTX-M-14, and CTX-M-27 is emerging in certain parts of the world. The genes encoding ESBLs are often found on plasmids and harboured within transposons or insertion sequences, which has enabled their spread. In addition, the population of ESBL-producing Escherichia coli is dominated globally by a highly virulent and successful clone belonging to ST131. Today, there are many diagnostic tools available to the clinical microbiology laboratory and include both phenotypic and genotypic tests to detect β-lactamases. Unfortunately, when ESBLs are not identified in a timely manner, appropriate antimicrobial therapy is frequently delayed, resulting in poor clinical outcomes. Several analyses of clinical trials have shown mixed results with regards to whether a carbapenem must be used to treat serious infections caused by ESBLs or whether some of the older β-lactam-β-lactamase combinations such as piperacillin/tazobactam are appropriate. Some of the newer combinations such as ceftazidime/avibactam have demonstrated efficacy in patients. ESBL-producing Gram-negative pathogens will continue to be major contributor to antimicrobial resistance worldwide. It is essential that we remain vigilant about identifying them both in patient isolates and through surveillance studies.

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“…This strain was sub-typed based on its fimH typing region, which is closely related and often associated to the H41 group of E. coli ST131 (1 SNP difference) [ 33 – 35 ] . To the best of our knowledge, there has been no outbreak report of this ST131 H89 E. coli subclone, which is anecdotally reported in population genomics [ 31 , 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of ESBL-producing Escherichia coli from repeated prevalence studies over 11 years in a long-term-care facility

Martischang

François

Cherkaoui

et al. 2021

Antimicrob Resist Infect Control

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Background Escherichia coli sequence type (ST) 131 H30 is an emerging multidrug resistant subclone, known to spread and cause outbreaks in long-term care facilities (LTCFs). Objectives and methods From 2010 through 2020, we performed 11 yearly surveillance studies for determining the prevalence of digestive carriage of ESBL-producing E. coli (ESBL-EC) among residents in a university-affiliated LCTF. Sequencing and genotyping of selected isolates were performed to characterize temporal trends in the prevalence and epidemic potential of ESBL-EC subclones, and for evaluating a potential rebound effect following discontinuation of contact precautions for ESBL-EC carriers in January 2019. Results This study included 2′403 LTCF residents, with 252 (10.5%) positive for ESBL-EC. Among the 236 ESBL-EC isolates available for typing, 58.0% belonged to the ST131 lineage, including 94/137 (68.6%) ST131 H30 isolates. An increasing yearly prevalence was observed for ESBL-EC (from 4.6 to 9.4%; p = 0.11), but not for the ST131 H30 subclone, which peaked in 2015 and declined thereafter. Multiple previously unnoticed ESBL-EC outbreaks occurred in the LTCF. Since 2018, we noted the clonal expansion of a rare ST131 H89 subclone (O16:H5) harboring CTX-M-14 and CTX-M-24. No rebound effect was observed in ESBL-EC prevalence nor in the different subclones following discontinuation of contact precautions for ESBL-EC carriers since 2019. Conclusion Clonal fluctuation was observed for ST131 H30 ESBL-EC with a current decline in prevalence. Surveillance should include the evolution of ST131 non-H30 subclones, which may spread in LTCFs. Our findings suggest that discontinuation of contact precautions for ESBL-EC carriers in LTCFs may be safely implemented, in support of European recommendations to limit ESBL-producing Enterobacteriaceae control measures in endemic settings to non-E. coli.

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Trends in Population Dynamics ofEscherichia coliSequence Type 131, Calgary, Alberta, Canada, 2006–20161

Cited by 29 publications

References 36 publications

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

Extended-spectrum β-lactamases: an update on their characteristics, epidemiology and detection

Epidemiology of ESBL-producing Escherichia coli from repeated prevalence studies over 11 years in a long-term-care facility

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