Trends, Fads and ART!

Dale, Brian; Ménézo, Yves; Coppola, G.

doi:10.1007/s10815-014-0420-z

Cited by 8 publications

(4 citation statements)

References 59 publications

(60 reference statements)

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“…Oocytes from women with polycystic ovary syndrome, while often appearing morphologically normal, are notoriously disorganized at the organelle level. Elsewhere, we have pointed out the heterogeneity of ovulated oocytes which, despite apparent synchrony in nuclear maturity, express asynchrony in cytoplasmic maturity (Dale et al, 2015).…”

Section: Clinical Concernsmentioning

confidence: 82%

Assisted yes, but where do we draw the line?

Santella

Dale²

2015

Reproductive BioMedicine Online

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In a recent report in Reproductive Biomedicine Online by Ebner et al., a comprehensive multi-centre study was presented on the use of a calcium ionophore, A23187, to artificially activate oocytes from patients who had poor fertilization rates in previous cycles. Under physiological conditions, the calcium increase in oocytes at activation is caused by influx and release from specific stores and ion channels, and has precise temporal, quantitative and spatial patterns. Calcium ionophores may release Ca(2+) in an uncontrolled fashion from intracellular stores that would not normally be involved in the activation process. Ionophores, including A23187, have a multitude of effects on cell homeostasis, not yet defined in oocytes, that may have long-term effects, for example on gene expression. We suspect that the successful births reported by Ebner et al. are a result of the overriding influence of the injected spermatozoa, rather than the effect of the ionophore; nevertheless, such an invasive non-physiological approach to assisted reproduction techniques is worrying, especially as epigenetic effects may result in future generations.

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Section: Clinical Concernsmentioning

confidence: 82%

Assisted yes, but where do we draw the line?

Santella

Dale²

2015

Reproductive BioMedicine Online

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“…Nonetheless, clinical implementation of the TLI culture system has allowed for an exponential evolution in embryo grading from conventional morphology grading to morphokinetic grading and finally integration of AI algorithms, a step towards automation [46][47][48][49][50][51][52]. Multiple studies investigating the potential benefit of multi-time point evaluation of morphokinetic parameters in embryos resulted in the growing interest and application of TLI and morphokinetic embryo grading [51,[53][54][55][56][57][58]. Consequently, embryo selection is currently based on a combination of morphological and kinetic parameters often utilising semi/automated and/ or AI algorithms such as KIDScore® (Vitrolife, Sweden) and Eeva® System (Merck group, Germany).…”

Section: Embryo Culture and Selectionmentioning

confidence: 99%

Automation in ART: Paving the Way for the Future of Infertility Treatment

et al. 2022

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In vitro fertilisation (IVF) is estimated to account for the birth of more than nine million babies worldwide, perhaps making it one of the most intriguing as well as commoditised and industrialised modern medical interventions. Nevertheless, most IVF procedures are currently limited by accessibility, affordability and most importantly multistep, labour-intensive, technically challenging processes undertaken by skilled professionals. Therefore, in order to sustain the exponential demand for IVF on one hand, and streamline existing processes on the other, innovation is essential. This may not only effectively manage clinical time but also reduce cost, thereby increasing accessibility, affordability and efficiency. Recent years have seen a diverse range of technologies, some integrated with artificial intelligence, throughout the IVF pathway, which promise personalisation and, at least, partial automation in the not-so-distant future. This review aims to summarise the rapidly evolving state of these innovations in automation, with or without the integration of artificial intelligence, encompassing the patient treatment pathway, gamete/embryo selection, endometrial evaluation and cryopreservation of gametes/embryos. Additionally, it shall highlight the resulting prospective change in the role of IVF professionals and challenges of implementation of some of these technologies, thereby aiming to motivate continued research in this field. Graphical abstract

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“…Elective single embryo transfer is a new technology in assisted reproduction (AR) that has contributed to an increase in pregnancy rate (Bissonnette et al , 2007). Therefore, identification of embryo quality predictors for reasonable embryo selection is of current importance (Dale et al , 2015; Albertini, 2016).…”

Section: Introductionmentioning

confidence: 99%

Cell and genetic predictors of human blastocyst hatching success in assisted reproduction

Syrkasheva

Долгушина

Romanov

et al. 2017

Zygote

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The aim was to identify cell and genetic predictors of human blastocyst hatching success in assisted reproduction programmes via a prospective case-control study. Blastocysts, donated by couples in assisted reproduction programmes were used. Hatching success assessment was performed after 144-146 h post-fertilization. The mRNA expression levels of cathepsin V (CTSV), GATA-binding protein 3 (GATA3) and human chorionic gonadotropin beta subunit 3, 5, 7 and 8 (CGB) genes were detected by quantitative real-time polymerase chain reaction. The odds ratio (OR) of hatching due to zona pellucida (ZP) thickness, oocyte and sperm quality, embryo quality and mRNA expression of CTSV, GATA3 and CGB genes in blastocysts was determined. From 62 blastocysts included in the study, 47 (75.8%) were unable to hatch spontaneously. The ZP thickening, and oocyte and sperm quality did not affect human blastocyst ability to hatch, except the combination of cytoplasmic and extracytoplasmic oocyte dysmorphisms (OR = 1.25; 95% confidence interval = 1.08, 1.45). Hatching-capable blastocysts had higher Gardner scale grade and mRNA expression of CTSV, GATA3 and CGB genes than hatching-incapable blastocysts. The human blastocyst hatching success depends on the blastocyst Gardner grade, but not on ZP and gamete quality. Blastocyst development was regulated by CTSV, GATA3 and CGB gene expression.

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Trends, Fads and ART!

Cited by 8 publications

References 59 publications

Assisted yes, but where do we draw the line?

Assisted yes, but where do we draw the line?

Automation in ART: Paving the Way for the Future of Infertility Treatment

Cell and genetic predictors of human blastocyst hatching success in assisted reproduction

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