Trends and recent development of the microelectrode arrays (MEAs)

Xu, Longqian; Hu, Chin-Hwa; Huang, Qi; Jin, Kai; Wang, Dongping; Hou, Wei; Dong, Lihua; Hu, Siyi; Ma, Hanbin

doi:10.1016/j.bios.2020.112854

Cited by 23 publications

(14 citation statements)

References 114 publications

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“…Utah-array-like devices (even if flexible) would cause too much damage to the pancreas, and are limited in depth compared to shank-based MEAs. Silicon or polymer shanks can be very long with electrodes placed along their length (Xu et al , 2021). The implantation of flexible MEAs is challenging but can be made possible by the use of insertion shuttles (Felix et al , 2013; Weltman, Yoo and Meng, 2016).…”

Section: Resultsmentioning

confidence: 99%

A flexible implant towards acute intrapancreatic electrophysiology

Pascual

Brauns

Domes

et al. 2023

Preprint

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Microelectrode arrays (MEAs) have proven to be a powerful tool to study electrophysiological processes over the last decades with most technology developed for investigation of the heart or brain. Other targets in the field of bioelectronic medicine are the peripheral nervous system and its innervation of various organs. Beyond the heart and nervous systems, the beta cells of the pancreatic islets of Langerhans generate action potentials during the production of insulin. In vitro experiments have demonstrated that their activity is a biomarker for blood glucose levels, suggesting that recording their activity in vivo could support patients suffering from diabetes mellitus with long-term automated read-out of blood glucose concentrations. Here, we present a flexible polymer-based implant having 64 low impedance microelectrodes designed to be implanted to a depth of 10 mm into the pancreas. As a first step, the implant will be used in acute experiments in pigs to explore the electrophysiological processes of the pancreas in vivo. Beyond use in the pancreas, our flexible implant and simple implantation method may also be used in other organs such as the brain.

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Section: Resultsmentioning

confidence: 99%

A flexible implant towards acute intrapancreatic electrophysiology

Pascual

Brauns

Domes

et al. 2023

Preprint

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“…With the advances in nano-fabrication, various miniaturized molecular sensing platforms have been fabricated for electrochemical gene detection [ 9 , 10 ]. These nanostructured devices are known for performing high throughput measurements, and having small resistor–capacitor constants and high mass-transfer rate [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Silver nano-reporter enables simple and ultrasensitive profiling of microRNAs on a nanoflower-like microelectrode array on glass

Gan

Zhou

et al. 2022

J Nanobiotechnol

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MicroRNAs (miRNAs) are small non-coding RNAs with ~ 22 nucleotides, playing important roles in the post-transcriptional regulation of gene expression. The expression profiles of many miRNAs are closely related to the occurrence and progression of cancer and can be used as biomarkers for cancer diagnosis and prognosis. However, their intrinsic properties, such as short length, low abundance and high sequence homology, represent great challenges in miRNA detection of clinical samples. To overcome these challenges, we developed a simple, ultrasensitive detection platform of electrochemical miRNAs chip (e-miRchip) with a novel signal amplification strategy using silver nanoparticle reporters (AgNRs) for multiplexed, direct, electronic profiling of miRNAs. A two-step hybridization strategy was used to detect miRNAs, where the target miRNA hybridizes with a stem-loop probe to unlock the probe first, and the opened stem-loop can further hybridize with AgNRs for signaling amplification. To enhance the detection sensitivity, the gold nanoflower electrodes (GNEs) were constructed in the microaperture arrays of the e-miRchips by electroplating. With the optimal size of the GNEs, the e-miRchip showed excellent performance for miR-21 detection with a detection limit of 0.56 fM and a linear range extended from 1 fM to 10 pM. The e-miRchip also exhibited good specificity in differentiating the 3-base mismatched sequences of the target miRNA. In addition, the e-miRchip was able to directly detect miR-21 expression in the total RNA extracts or cell lysates collected from lung cancer cells and normal cells. This work demonstrated the developed e-miRchip as an efficient and promising miniaturized point-of-care diagnostic device for the early diagnosis and prognosis of cancers. Graphical Abstract

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“…In fact, in the last 2 decades, the fast growth of organic electronics, and in particular of organic transistor-based sensors and biosensors, has led to the development of a new generation of measuring devices for cellular applications, including those for electrophysiological and metabolic activity monitoring, as recently reported in ( Spanu et al, 2021a ). This new technology brings about several advantages with respect to the previously mentioned approaches, like the reduction of production costs and the possibility to employ materials such as plastic or paper, thus making it possible to design flexible devices with large area fabrication methods ( Xu et al, 2021b ). In particular, there have been recently some attempts of using mainly Organic Electrochemical Transistors (OECTs) for the in vitro detection of the electrical ( Gu et al, 2019 ) and metabolic ( Pappa et al, 2018 ; Moldero et al, 2020 ; Cacocciola et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous recording of electrical and metabolic activity of cardiac cells in vitro using an organic charge modulated field effect transistor array

Spanu

Martines

Tedesco

et al. 2022

Front. Bioeng. Biotechnol.

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In vitro electrogenic cells monitoring is an important objective in several scientific and technological fields, such as electrophysiology, pharmacology and brain machine interfaces, and can represent an interesting opportunity in other translational medicine applications. One of the key aspects of cellular cultures is the complexity of their behavior, due to the different kinds of bio-related signals, both chemical and electrical, that characterize these systems. In order to fully understand and exploit this extraordinary complexity, specific devices and tools are needed. However, at the moment this important scientific field is characterized by the lack of easy-to-use, low-cost devices for the sensing of multiple cellular parameters. To the aim of providing a simple and integrated approach for the study of in vitro electrogenic cultures, we present here a new solution for the monitoring of both the electrical and the metabolic cellular activity. In particular, we show here how a particular device called Micro Organic Charge Modulated Array (MOA) can be conveniently engineered and then used to simultaneously record the complete cell activity using the same device architecture. The system has been tested using primary cardiac rat myocytes and allowed to detect the metabolic and electrical variations thar occur upon the administration of different drugs. This first example could lay the basis for the development of a new generation of multi-sensing tools that can help to efficiently probe the multifaceted in vitro environment.

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Trends and recent development of the microelectrode arrays (MEAs)

Cited by 23 publications

References 114 publications

A flexible implant towards acute intrapancreatic electrophysiology

A flexible implant towards acute intrapancreatic electrophysiology

Silver nano-reporter enables simple and ultrasensitive profiling of microRNAs on a nanoflower-like microelectrode array on glass

Simultaneous recording of electrical and metabolic activity of cardiac cells in vitro using an organic charge modulated field effect transistor array

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