Trends and opportunities in food pathogen detection

Nugen, Sam R.; Baeumner, Antje J.

doi:10.1007/s00216-008-1886-2

Cited by 119 publications

(63 citation statements)

References 28 publications

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“…Despite the advances in the development of the rapid detection of foodborne bacteria, there remain significant challenges for the improvement of the separation step. Without the ability to physically separate and concentrate the bacteria from the food matrix, many of these new detection techniques cannot be put into practice (6,39,49). Additionally, possible inhibitors, particulates, and competing chemistries can make conducting rapid and sensitive pathogen detection in food matrix difficult.…”

Section: Introductionmentioning

confidence: 99%

“…Currently the food and agricultural industries rely mostly on standard microbiological methods to detect the presence of bacteria (53). However, the complexity of foods makes the sample preparation difficult and raises the possibility of false negative results (39,49). Another obstacle to pathogen separation is that bacterial cells can form relatively strong attachments to the surface and interior of the food which also makes separation from the matrix much more complex (16).…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic Digestion for Improved Bacteria Separation from Leafy Green Vegetables

Wang

et al. 2016

Journal of Food Protection

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An effective and rapid method for the separation of bacteria from food matrix remains a bottleneck for rapid bacteria detection for food safety. Bacteria can strongly attach to a food surface or internalize within the matrix, making their isolation extremely difficult. Traditional methods of separating bacteria from food routinely involve stomaching, blending, and shaking. However, these methods may not be efficient at removing all the bacteria from complex matrices. Here, we investigate the benefits of using enzyme digestion followed by immunomagnetic separation to isolate Salmonella from spinach and lettuce. Enzymatic digestion using pectinase and cellulase was able to break down the structure of the leafy green vegetables, resulting in the detachment and release of Salmonella from the leaves. Immunomagnetic separation of Salmonella from the liquefied sample allowed an additional separation step to achieve a more pure sample without leaf debris that may benefit additional downstream applications. We have investigated the optimal combination of pectinase and cellulase for the digestion of spinach and lettuce to improve sample detection yields. The concentrations of enzymes used to digest the leaves were confirmed to have no significant effect on the viability of the inoculated Salmonella. Results reported that the recovery of the Salmonella from the produce after enzyme digestion of the leaves was significantly higher (P <0.05) than traditional sample preparation methods to separate bacteria (stomaching and manually shaking). The results demonstrate the potential for use of enzyme digestion prior to separation can improve the efficiency of bacteria separation and increase the likelihood of detecting pathogens in the final detection assay.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzymatic Digestion for Improved Bacteria Separation from Leafy Green Vegetables

Wang

et al. 2016

Journal of Food Protection

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“…They are based on either chromogenic media [14][15][16], antibodies [17][18][19], or nucleic acid-based techniques [20,21]. Identification of Listeria spp.…”

Section: Introductionmentioning

confidence: 99%

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food

Blažková

Koets

Rauch

et al. 2009

Eur Food Res Technol

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We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with two labelled primer sets, one generic and directed to a specific sequence of the gene encoding 16S rRNA from Listeria spp. and the other specific and directed to a part of the prfA gene encoding the central virulence gene regulator from the food pathogen Listeria monocytogenes (3.5 h). The PCR solution is directly added to the one-step assay device and the appearance of a grey/black line is indicative of the presence of specific amplicons (max 15 min). In all tests performed, the method correctly identified L. monocytogenes and strains of Listeria spp. PCR material of over 20 food samples was tested by NALFIA. The method proved to be useful for the detection of L. monocytogenes in different kinds of food samples.

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“…Afin de diminuer le temps d'obtention des résultats ainsi que les équipements requis pour la détection moléculaire de pathogènes, les méthodes LAMP (loop-mediated isothermal amplification) et NASBA (nucleic acid sequence based amplification), basées sur l'amplification isotherme d'acides nucléiques, ont été développées (KARANIS et al, 2007;HEIJNEN et MEDEMA, 2009). Un module de biocapteur microfluidique permet la détection d'organismes pathogènes (Escherichia coli, les spores de Bacillus anthracis, les oocystes de Cryptosporidium parvum) en seulement 15 à 20 minutes (BAEUMNER et al, 2003;NUGEN et BAEUMNER, 2008;zAyTSEvA et al, 2005). De plus, une plateforme automatisée a été mise au point, permettant ainsi la concentration, la purification et la détection de pathogènes par PCR (STRAUB et al, 2005).…”

Section: Le Développement Des Techniques De Biologie Moléculaireunclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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L’émergence de pathogènes dans l’eau destinée à la consommation humaine représente une préoccupation majeure en matière de santé publique pour les industriels et les pouvoirs publics concernés. Parmi ces pathogènes, certains sont d’origine fécale (Cryptosporidium, Campylobacter ou bien les rotavirus), alors que d’autres vivent dans l’environnent naturel (Legionella, Pseudomonas, Aeromonas ou bien les mycobactéries). Dans l’optique de mettre en place une analyse des risques liés à la présence de ces pathogènes, il est important d’accroître nos connaissances sur l’écologie de ces microorganismes et de développer des outils d’analyse afin de réaliser une meilleure surveillance sanitaire. Par conséquent, l’écologie microbienne du réseau de distribution d’eau potable doit être étudiée en détail, particulièrement vis-à-vis des propriétés physiologiques et la diversité des espèces microbiennes présentes, afin de mieux comprendre les interactions entre les espèces communément rencontrées et celles pathogènes.The emergence of pathogens in water intended for human consumption is a major concern in terms of the public health industry and the public authorities concerned. Among these pathogens, some are of faecal origin (Cryptosporidium, Campylobacter, or rotavirus), while others live in the natural environment (Legionella, Pseudomonas, Aeromonas or mycobacteria). In order to establish a risk analysis related to the presence of these pathogens, it is important to increase our knowledge on the ecology of these microorganisms and to develop analytical tools to achieve better health monitoring. Therefore, the microbial ecology of drinking water distribution networks must be studied in detail, especially with respect to the properties and physiological diversity of the microbial species present, in order to better understand the interactions between species commonly encountered and those that are pathogens

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Trends and opportunities in food pathogen detection

Cited by 119 publications

References 28 publications

Enzymatic Digestion for Improved Bacteria Separation from Leafy Green Vegetables

Enzymatic Digestion for Improved Bacteria Separation from Leafy Green Vegetables

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

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