Trehalose synthesis in Saccharomycopsis fibuligera does not respond to stress treatments

Lu, Liang; Wang, X. K.; Zhu, Kailing; M., Z.

doi:10.1007/s00253-006-0688-8

Cited by 7 publications

(2 citation statements)

References 18 publications

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“…fibuligera β-actin gene(Genbank Accession No. DQ420644;Liang et al, 2007). The sequences of the probes are listed inTable 2.Software ImageJ was used for quantification of the hybridization signal.…”

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confidence: 99%

Glycerol accumulation in the dimorphic yeast Saccharomycopsis fibuligera: cloning of two glycerol 3‐phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress

Hua

Jia

Lin

et al. 2008

Yeast

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Glycerol plays an important role in the osmoadaptation responses of Saccharomyces cerevisiae. However, there is no detailed investigation about the role of glycerol in the osmoadaptation responses of Saccharomycopsis fibuligera. Here we show that both intra-and extracellular glycerol concentrations in Sm. fibuligera cells responded very quickly when they were subjected to osmotic stress. We then cloned two isogenes encoding putative NAD + -dependent glycerol 3-phosphate dehydrogenase (GPD) from Sm. fibuligera PD70 by degenerate PCR and subsequent chromosome walking methods. Those two genes, designated SfGPD1 and SfGPD2, respectively, exhibited 86.6% pairwise identity in their encoding regions, while there was no obvious homology in their non-coding regions. Either SfGPD1 or SfGPD2 could complement the salt tolerance characteristics of the gpd1gpd2 double mutant strain of S. cerevisiae, further demonstrating that both of those genes are functional homologues of S. cerevisiae GPD1 and GPD2. Northern blot analysis revealed that SfGPD1 was induced markedly by osmotic stress, while SfGPD2 was not. In consistency with the observation that there was no obvious glycerol content change when the cells were transferred to anoxic conditions, neither SfGPD1 nor SfGPD2 was induced when the cells were transferred to anoxic conditions, thus suggesting a functional splitting of glycerol 3-phosphate dehydrogenase between S. cerevisiae and Sm. fibuligera. The Genbank Accession Nos for SfGPD1 and SfGPD2 were DQ536095 and DQ536096, respectively.

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confidence: 99%

Glycerol accumulation in the dimorphic yeast Saccharomycopsis fibuligera: cloning of two glycerol 3‐phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress

Hua

Jia

Lin

et al. 2008

Yeast

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“…Trehalose-6-phosphate synthase (Tps1) and trehalase activities (Ath1 and Nth1) were determined as reported previously [26]–[29]. One unit of Tps1 activity was defined as the amount of enzyme that produces 1.0 µmol of NAD + per minute at 37°C and pH 6.6 [26], [29]. Glucose concentration in the supernates was measured using the glucose oxidase/peroxidase assay.…”

Section: Methodsmentioning

confidence: 99%

A Novel Strategy to Construct Yeast Saccharomyces cerevisiae Strains for Very High Gravity Fermentation

et al. 2012

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Very high gravity (VHG) fermentation is aimed to considerably increase both the fermentation rate and the ethanol concentration, thereby reducing capital costs and the risk of bacterial contamination. This process results in critical issues, such as adverse stress factors (ie., osmotic pressure and ethanol inhibition) and high concentrations of metabolic byproducts which are difficult to overcome by a single breeding method. In the present paper, a novel strategy that combines metabolic engineering and genome shuffling to circumvent these limitations and improve the bioethanol production performance of Saccharomyces cerevisiae strains under VHG conditions was developed. First, in strain Z5, which performed better than other widely used industrial strains, the gene GPD2 encoding glycerol 3-phosphate dehydrogenase was deleted, resulting in a mutant (Z5ΔGPD2) with a lower glycerol yield and poor ethanol productivity. Second, strain Z5ΔGPD2 was subjected to three rounds of genome shuffling to improve its VHG fermentation performance, and the best performing strain SZ3-1 was obtained. Results showed that strain SZ3-1 not only produced less glycerol, but also increased the ethanol yield by up to 8% compared with the parent strain Z5. Further analysis suggested that the improved ethanol yield in strain SZ3-1 was mainly contributed by the enhanced ethanol tolerance of the strain. The differences in ethanol tolerance between strains Z5 and SZ3-1 were closely associated with the cell membrane fatty acid compositions and intracellular trehalose concentrations. Finally, genome rearrangements in the optimized strain were confirmed by karyotype analysis. Hence, a combination of genome shuffling and metabolic engineering is an efficient approach for the rapid improvement of yeast strains for desirable industrial phenotypes.

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UV-B Irradiation Regulates Apoptosis in Yeast

Chen

Liang

Yang

et al. 2013

Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

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Trehalose synthesis in Saccharomycopsis fibuligera does not respond to stress treatments

Cited by 7 publications

References 18 publications

Glycerol accumulation in the dimorphic yeast Saccharomycopsis fibuligera: cloning of two glycerol 3‐phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress

Glycerol accumulation in the dimorphic yeast Saccharomycopsis fibuligera: cloning of two glycerol 3‐phosphate dehydrogenase genes, one of which is markedly induced by osmotic stress

A Novel Strategy to Construct Yeast Saccharomyces cerevisiae Strains for Very High Gravity Fermentation

UV-B Irradiation Regulates Apoptosis in Yeast

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