Trehalose pretreatment induces drought tolerance in radish (Raphanus sativus L.) plants: some key physio-biochemical traits

Akram, Nudrat Aisha; Waseem, Muhammad; Ameen, Rabia; Ashraf, Muhammad

doi:10.1007/s11738-015-2018-1

Cited by 58 publications

(41 citation statements)

References 45 publications

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“…During this experiment, the activities of SOD, POD and CAT were found to increase with increasing levels of drought. These results are similar to other reports on different plants such as maize (Moussa & Abdel-Aziz 2008), radish (Akram et al 2016) and corn (Darvishan et al 2013).…”

Section: Discussionsupporting

confidence: 92%

“…In this study, chlorophyll b content decreased in spinach plants when they were subjected to varying levels of drought stress. Previously, Akram et al (2016) in radish and Yasmeen et al (2013) in wheat observed that drought stress decreased the contents of chlorophyll b where they attributed this decrease in chlorophyll pigments to waterstress induced damages to oxidative defense system. Chlorophyll b is a premier light harvesting pigment, and relatively more drought-induced reduction in chlorophyll b content compared to chlorophyll a could have been due to the reason that water deficiency adversely affected the PS-I, light harvesting/capturing system, energy transfer and antenna complex accessories (Ashraf & Harris 2013).…”

Section: Discussionmentioning

confidence: 92%

“…Various studies have reported that high accumulation of osmoprotectants is interlinked with better survival of plants under stress conditions. For example, Akram et al (2016) and Galahitigama and Wathugala (2016) in rice in radish plants observed improvement in stress tolerance due to an increase in proline and GB contents under water deficit conditions. Stress-induced oxidative stress causes the generation of a myriad of ROS which cause damage to several key biomolecules including bio-membranes of vital cellular organelles.…”

Section: Discussionmentioning

confidence: 98%

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Assessment of Biochemical Changes in Spinach (Spinacea oleracea L.) Subjected to Varying Water Regimes

Jabeen¹,

Akram²,

Ashraf³

et al. 2019

JSM

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It is known that leafy vegetables including spinach (Spinacea oleracea L.) contain relatively high amount of water, therefore, their water requirement during the life cycle is comparatively more than the other vegetables. In addition, there is an association between osmoprotection and antioxidants with reference to drought stress tolerance. Keeping in mind these facts, the present study was conducted to assess the changes in plant growth, osmoprotectants, chlorophyll pigments and activities/levels of antioxidative system in spinach (Spinacea oleracea L.) grown under varying water deficit regimes with 40%, 60%, 80% and 100% field capacity (FC). Imposition of varying water regimes significantly decreased shoot and root fresh and dry weights, shoot plus root lengths, and chlorophyll b contents of spinach plants. Increase in proline, glycinebetaine (GB), total phenolics, ascorbic acid and malondialdehyde (MDA) contents as well as the activities of antioxidant enzymes including superoxide dismutase, peroxidase and catalase were observed in the spinach plants particularly at 40% FC. The most effective level of water stress for elevating the proline, GB and antioxidant levels/ activities was observed at 40% FC followed by 60% FC. Hence, the results of this study suggested that upregulation of antioxidants and osmoprotectants is positively associated with the drought tolerance of spinach which depends on the severity of water stress level. These results can be used to narrow the gap between selection of plant species and requirement of irrigated water for the crops grown on dry land areas. ABSTRAKAdalah diketahui bahawa sayur-sayuran berdaun termasuk bayam (Spinacea oleracea L.) mengandungi jumlah air yang agak tinggi, oleh itu, mereka memerlukan lebih air sepanjang kitaran hidup berbanding sayur-sayuran lain. Di samping itu, terdapat hubungan antara osmoperlindungan dan antioksidan berkaitan toleransi tekanan kemarau. Dengan mengambil kira fakta tersebut, kajian ini dijalankan untuk menilai perubahan dalam pertumbuhan tanaman, osmoperlindungan, pigmen klorofil serta aktiviti/tahap sistem antioksidatif pada bayam (Spinacea oleracea L.) yang ditanam di bawah rejim defisit air berbeza dengan 40%, 60%, 80% dan 100% kapasiti lapangan (FC). Pengenaan rejim air yang berbeza dengan ketara mengurangkan berat segar dan kering pucuk dan akar, panjang pucuk dan akar serta kandungan klorofil b pada pokok bayam. Pertambahan kandungan prolin, glisinbetain (GB), jumlah fenolik, asid askorbik dan malondialdehid (MDA) serta aktiviti enzim antioksida termasuk superoksida dismutase, peroksidase dan katalase telah diperhatikan pada tanaman bayam terutamanya pada 40% FC. Tahap tekanan air paling berkesan untuk meningkatkan tahap/aktiviti proline, GB dan antioksidan diperhatikan pada 40% FC diikuti 60% FC. Oleh itu, keputusan kajian ini mencadangkan pengawalaturan atas antioksidan dan osmoperlindungan dikaitkan secara positif dengan toleransi kemarau oleh bayam yang bergantung pada keterukan aras tekanan air. Keputusan ini boleh digunakan unt...

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 98%

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Assessment of Biochemical Changes in Spinach (Spinacea oleracea L.) Subjected to Varying Water Regimes

Jabeen¹,

Akram²,

Ashraf³

et al. 2019

JSM

Self Cite

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“…In potato, leaf chlorophyll content was used as an efficient indicator of tuber yield under drought condition (Ramirez et al, 2014). Recent findings in some vegetable crops like eggplant (Mibei et al, 2017) and raddish (Akram et al, 2016) further supports our finding where drought stress reduces the chlorophyll content however the tolerant cultivar maintains the higher chlorophyll and thereby the photosynthesis rate as one of the drought tolerance mechanism. Drought stress induces the formation of detrimental reactive oxygen species (ROS) that directly damages the cellular membrane that affects the various cellular metabolic processes (Das and Roychoudhury, 2014).…”

Section: Physiological and Biochemical Traitssupporting

confidence: 83%

Physiological and Biochemical Responses in Onion Crop to Drought Stress

Ghodke¹,

Andhale²,

Gijare³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…Similar observation was reported by Horita and Saruyama (2006), wherein onion ( Allium cepa L.) seeds treated with 400 mM trehalose stimulated a rapid and uniform seed germination under normal conditions. More recently, Akram et al (2016) demonstrated that seed treatment with 25 mM trehalose significantly promoted radish ( Raphanus sativus L.) fresh and dry biomasses, as well as chlorophyll a and total soluble sugar contents in the radish plants. These results potentiate the important roles of exogenous trehalose treatment in seed germination and seedling vigor under normal conditions.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

et al. 2016

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In recent years, diverse physiological functions of various sugars are the subject of investigations. Their roles in signal transduction in plant responses to adverse biotic and abiotic stress conditions have become apparent, and growing scientific evidence has indicated that disaccharides like sucrose and trehalose mediate plant defense responses in similar way as those induced by elicitors against the pathogens. Trehalose is a well-known metabolic osmoregulator, stress-protectant and non-reducing disaccharide existing in a variety of organisms, including fungi, bacteria, and plants. Commercially procured trehalose was applied to seeds of susceptible pearl millet (Pennisetum glaucum) cultivar “HB3,” and tested for its ability to reduce downy mildew disease incidence by induction of resistance. Seed treatment with trehalose at 200 mM for 9 h recorded 70.25% downy mildew disease protection, followed by those with 100 and 50 mM trehalose which offered 64.35 and 52.55% defense, respectively, under greenhouse conditions. Furthermore, under field conditions treatment with 200 mM trehalose for 9 h recorded 67.25% downy mildew disease protection, and reduced the disease severity to 32.75% when compared with untreated control which displayed 90% of disease severity. Trehalose did not affect either sporangial formation or zoospore release from sporangia, indicating that the reduction in disease incidence was not due to direct inhibition but rather through induction of resistance responses in the host. Additionally, trehalose was shown to enhance the levels of polyphenol oxidase, phenylalanine ammonia lyase, and peroxidase, which are known as markers of both biotic and abiotic stress responses. Our study shows that osmoregulators like trehalose could be used to protect plants against pathogen attacks by seed treatment, thus offering dual benefits of biotic and abiotic stress tolerance.

show abstract

Trehalose pretreatment induces drought tolerance in radish (Raphanus sativus L.) plants: some key physio-biochemical traits

Cited by 58 publications

References 45 publications

Assessment of Biochemical Changes in Spinach (Spinacea oleracea L.) Subjected to Varying Water Regimes

Assessment of Biochemical Changes in Spinach (Spinacea oleracea L.) Subjected to Varying Water Regimes

Physiological and Biochemical Responses in Onion Crop to Drought Stress

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

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