Trehalose preincubation increases mesenchymal (CD271⁺) stem cells post-cryopreservation viability

Abstract

ABSTRAK ABSTRACTBackground: Dimethyl sulfoxide (Me2SO) is a common cryoprotective agent widely used in cell preservation system. Me2SO is currently known to cause epigenetic changes which are critical in stem cells development and cellular differentiation. Therefore, it is imperative to develop cryopreservation techniques that protect cellular functions and avert Me2SO adverse effect. Trehalose was able to protect organism in extreme condition such as dehydration and cold. This study aimed to verify the protective effect of trehalose preincubation procedure in cryopreservation.

Advances and challenges in umbilical cord blood and tissue bioprocessing: procurement and storage

“…DMSO readily permeates cell membranes and impedes extracellular ice formation by depressing the freezing point of water, and polysaccharides are thought to assist by confounding the formation of intracellular ice [111]. The addition of polysaccharide solutions also reduces the concentration of DMSO necessary for successful cryopreservation [106,112]. Hence, we recommend that the minimum concentration of DMSO required, validated with respect to optimal post-thaw cell recovery, in conjunction with the nontoxic additives previously listed, be used to cryopreserve UCT and UCB for translation/clinical application.…”

Benefit of trehalose preincubation prior to cryopreservation

“…However, this study only addressed viability after 24 hours in liquid nitrogen, 1 and did not address other functional variables that play a role in the success of cell therapy, which used cryopreserved cells, such as differentiation capacity, clonogenic efficiency, immune-modulation properties, and senescence after thawing.…”