Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model

Manai, Federico; Azzalin, Alberto; Morandi, Martina; Riccardi, Veronica; Zanoletti, Lisa; Giudici, Marco Dei; Gabriele, Fabio; Martinelli, Carolina; Bozzola, Mauro; Comincini, Sergio

doi:10.3390/cells8040348

Cited by 9 publications

(11 citation statements)

References 49 publications

(68 reference statements)

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“…Whole protein extraction and immunoblotting analysis was performed as previously described [26]. In detail, T98G cell pellets were resuspended in ice-cold RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 1 mM Triton X100, all from Sigma-Aldrich), supplemented with Complete Mini protease inhibitor cocktail (Roche, Basel, Switzerland).…”

Section: Immunoblotting Analysismentioning

confidence: 99%

Berberine Photo-Activation Potentiates Cytotoxicity in Human Astrocytoma Cells through Apoptosis Induction

Morra

Martinelli

Gabriele

et al. 2021

JPM

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Photodynamic therapy (PDT) has recently attracted interest as an innovative and adjuvant treatment for different cancers including malignant gliomas. Among these, Glioblastoma (GBM) is the most prevalent neoplasm in the central nervous system. Despite conventional therapeutic approaches that include surgical removal, radiation, and chemotherapy, GBM is characterized by an extremely poor prognosis and a high rate of recurrence. PDT is a physical process that induces tumor cell death through the genesis and accumulation of reactive oxygen species (ROS) produced by light energy interaction with a photosensitizing agent. In this contribution, we explored the potentiality of the plant alkaloid berberine (BBR) as a photosensitizing and cytotoxic agent coupled with a PDT scheme using a blue light source in human established astrocytoma cell lines. Our data mainly indicated for the combined BBR-PDT scheme a potent activation of the apoptosis pathway, through a massive ROS production, a great extent of mitochondria depolarization, and the sub-sequent activation of caspases. Altogether, these results demonstrated that BBR is an efficient photosensitizer agent and that its association with PDT may be a potential anticancer strategy for high malignant gliomas.

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Section: Immunoblotting Analysismentioning

confidence: 99%

Berberine Photo-Activation Potentiates Cytotoxicity in Human Astrocytoma Cells through Apoptosis Induction

Morra

Martinelli

Gabriele

et al. 2021

JPM

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“…Trehalose is a natural disaccharide, which is usually used as a medical desiccant. Nowadays, it has attracted much attention as a mTOR-independent autophagy inducer [ 12 , 174 ]. DeBosch et al [ 175 ] observed that Trehalose prevented cells from taking up glucose via blocking glucose transporters in the plasma membrane.…”

Section: Liver Steatosismentioning

confidence: 99%

Modulation of Autophagy: A Novel “Rejuvenation” Strategy for the Aging Liver

Tautenhahn

Dirsch

et al. 2021

Oxidative Medicine and Cellular Longevity

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Aging is a natural life process which leads to a gradual decline of essential physiological processes. For the liver, it leads to alterations in histomorphology (steatosis and fibrosis) and function (protein synthesis and energy generation) and affects central hepatocellular processes (autophagy, mitochondrial respiration, and hepatocyte proliferation). These alterations do not only impair the metabolic capacity of the liver but also represent important factors in the pathogenesis of malignant liver disease. Autophagy is a recycling process for eukaryotic cells to degrade dysfunctional intracellular components and to reuse the basic substances. It plays a crucial role in maintaining cell homeostasis and in resisting environmental stress. Emerging evidence shows that modulating autophagy seems to be effective in improving the age-related alterations of the liver. However, autophagy is a double-edged sword for the aged liver. Upregulating autophagy alleviates hepatic steatosis and ROS-induced cellular stress and promotes hepatocyte proliferation but may aggravate hepatic fibrosis. Therefore, a well-balanced autophagy modulation strategy might be suitable to alleviate age-related liver dysfunction. Conclusion. Modulation of autophagy is a promising strategy for “rejuvenation” of the aged liver. Detailed knowledge regarding the most devastating processes in the individual patient is needed to effectively counteract aging of the liver without causing obvious harm.

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“…The viability and apoptotic rates of glioma cells were quantified using the Muse Count & Viability or Annexin V and Dead Cell assays (Luminex, Austin, TX, USA), as described [40]. After trypsinization and collection, cells were washed 3 times with D-PBS, resuspended in D-PBS + 1% FBS (v/v) and 1 volume each of Count & Viability or Annexin V reagents, and incubated for 5 or 20 min at room temperature in the dark, respectively.…”

Section: Viability and Apoptosis Cytofluorimetric Analysismentioning

confidence: 99%

Development of Artificial Plasma Membranes Derived Nanovesicles Suitable for Drugs Encapsulation

Martinelli

Gabriele

Dini

et al. 2020

Cells

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Extracellular vesicles (EVs) are considered as promising nanoparticle theranostic tools in many pathological contexts. The increasing clinical employment of therapeutic nanoparticles is contributing to the development of a new research area related to the design of artificial EVs. To this aim, different approaches have been described to develop mimetic biologically functional nanovescicles. In this paper, we suggest a simplified procedure to generate plasma membrane-derived nanovesicles with the possibility to efficiently encapsulate different drugs during their spontaneously assembly. After physical and molecular characterization by Tunable Resistive Pulse Sensing (TRPS) technology, transmission electron microscopy, and flow cytometry, as a proof of principle, we have loaded into mimetic EVs the isoquinoline alkaloid Berberine chloride and the chemotherapy compounds Temozolomide or Givinostat. We demonstrated the fully functionality of these nanoparticles in drug encapsulation and cell delivery, showing, in particular, a similar cytotoxic effect of direct cell culture administration of the anticancer drugs. In conclusion, we have documented the possibility to easily generate scalable nanovesicles with specific therapeutic cargo modifications useful in different drug delivery contexts.

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Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model

Cited by 9 publications

References 49 publications

Berberine Photo-Activation Potentiates Cytotoxicity in Human Astrocytoma Cells through Apoptosis Induction

Berberine Photo-Activation Potentiates Cytotoxicity in Human Astrocytoma Cells through Apoptosis Induction

Modulation of Autophagy: A Novel “Rejuvenation” Strategy for the Aging Liver

Development of Artificial Plasma Membranes Derived Nanovesicles Suitable for Drugs Encapsulation

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