2021
DOI: 10.3389/fcimb.2021.670935
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Trehalose-Induced Remodelling of the Human Microbiota Affects Clostridioides difficile Infection Outcome in an In Vitro Colonic Model: A Pilot Study

Abstract: Within the human intestinal tract, dietary, microbial- and host-derived compounds are used as signals by many pathogenic organisms, including Clostridioides difficile. Trehalose has been reported to enhance virulence of certain C. difficile ribotypes; however, such variants are widespread and not correlated with clinical outcomes for patients suffering from C. difficile infection (CDI). Here, we make preliminary observations on how trehalose supplementation affects the microbiota in an in vitro model and show … Show more

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Cited by 22 publications
(27 citation statements)
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References 66 publications
(84 reference statements)
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“…Bacterial populations were sampled daily in each of the three models, in which a 10-fold dilution series of this sample was plated out onto various selective and non-selective agars for enumeration as described previously ( Buckley et al, 2021 ). Bacterial colonies were enumerated and confirmed using microbiological and biochemical techniques.…”
Section: Methodsmentioning
confidence: 99%
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“…Bacterial populations were sampled daily in each of the three models, in which a 10-fold dilution series of this sample was plated out onto various selective and non-selective agars for enumeration as described previously ( Buckley et al, 2021 ). Bacterial colonies were enumerated and confirmed using microbiological and biochemical techniques.…”
Section: Methodsmentioning
confidence: 99%
“…The concentration of each antibiotic was determined by bioassays as previously described ( Buckley et al, 2021 ). Briefly, to determine the concentration of sarecycline and minocycline, indicator organism Kocuria rhizophila (ATCC 9341) was inoculated into Wilkins-Chalgren agar plates.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Trehalose was found superior in preserving bacterial viability, with membrane integrity tests comparable to frozen products ( Staley et al., 2017a ), and it appears to work consistently among other groups pursuing lyophilized encapsulated microbiota ( Xu et al., 2021 ). While the use of trehalose has been suggested to enhance the virulence of some strains of C. difficile ( Collins et al., 2018 ), there are several studies that show trehalose supplementation does not interfere with the clinical efficacy of cFMT ( Saund et al., 2020 ; Buckley et al., 2021 ).…”
Section: Development Of the Capsule Productmentioning
confidence: 99%
“…It will, however, be more difficult to define the role of biofilms in recurrent CDI. Recent studies provide evidence that biofilm formation is dependent on metabolism and excreted metabolites [ 192 , 270 ]. Therefore, we think there is a need to refine our view on the infectious cycle of C. difficile .…”
Section: Persistence In the Gut: Spores Biofilms Or Both?mentioning
confidence: 99%