Trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in Nili Ravi buffaloes ( Bubalus bubalis )

Iqbal, Sajid; Andrabi, S. M. H.; Riaz, Amjad; Durrani, Aneela Zameer; Ahmad, Nasim

doi:10.1016/j.theriogenology.2015.11.004

Cited by 48 publications

(22 citation statements)

References 36 publications

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“…ROS have been known for decades to be a detrimental factor in many physiological and pathological processes. This includes a strong correlation between ROS production and cancer, cardiovascular disease, diabetic neuropathy and infertility (Iqbal, Andrabi, Riaz, Durrani, & Ahmad, 2016; Motlagh et al, 2014; Sarıözkan et al, 2015). Macleod first reported that ROS production arose in spermatozoa and that this increase in the partial pressure of oxygen would reduce sperm motility (MacLeod, 1943b).…”

Section: Discussionmentioning

confidence: 99%

Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress—A pilot study

Khashavi¹,

Homaei

Koohnavard

et al. 2020

Andrologia

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Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze–thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS‐scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500 µg/ml of H. parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p < .05). The addition of 250 µg/ml of the extract exerted a significant positive effect on sperm motility. Moreover, sperm DNA damage and ROS production were significantly reduced at extract concentrations of 250 and 500 µg/ml (p < .05). To the best of our knowledge, the results of this study represent the first demonstration of the possibility of improving sperm parameters and reducing ROS production and DNA damage by supplementing sperm freezing media with H. parva coelomic extract. Our results suggested that H. parva coelomic extract could be useful for improving the fertilising ability of frozen‐thawed human semen.

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Section: Discussionmentioning

confidence: 99%

Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress—A pilot study

Khashavi¹,

Homaei

Koohnavard

et al. 2020

Andrologia

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show abstract

“…It is well known that there is an overproduction of ROS because of oxidative stress during semen processing and cryopreservation (Andrabi, ). Addition of antioxidants in semen extender controls the degree of ROS generation during cryopreservation (Iqbal et al., , b). At post‐dilution, we found higher seminal total antioxidant content with all the concentrations of curcumin added in extender compared to control.…”

Section: Discussionmentioning

confidence: 99%

“…It is well documented that ROS formed by the univalent reduction of oxygen (Bilodeau, Chatterjee, Sirad, & Gagnon, ) are responsible for maintenance of physiological processes in spermatozoa, that is capacitation and the acrosome reaction (O'Flaherty, Beconi, & Beorlegui, ). Therefore, the balance between ROS generation and antioxidants should be precise for the protection of spermatozoa (Iqbal, Andrabi, Riaz, Durrani, & Ahmad, ).…”

Section: Introductionmentioning

confidence: 99%

Freezability of water buffalo bull (Bubalus bubalis ) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender

Shah

Andrabi²,

Qureshi

2016

Andrologia

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Effects of curcumin as antioxidant in extender were evaluated on freezability of buffalo spermatozoa. Semen from each of the five bulls (n = 3 replicates, six ejaculates/bull, a total of 30 ejaculates) was diluted in Tris-citric acid extender containing curcumin (0.5, 1.0, 1.5 or 2.0 mM) or control. At pre-freezing and post-thawing, total antioxidant contents (μM/L) and lipid peroxidation levels (μM/ml) were higher (p < .05) and lower (p < .05) respectively, with 1.5 and 2.0 mM compared to 0.5 and 1.0 mM curcumin and control. At post-thawing, progressive motility (PM, %) and rapid velocity (RV, %) were higher (p < .05) with 1.5 mM compared to other doses of curcumin and control (except in case of RV, 1.5 was similar with 1.0 mM). Kinematics (average path velocity, μm/s; straight-line velocity, μm/s; curved-line velocity, μm/s; straightness, %; linearity, %), in vitro longevity (%, PM and RV) and DNA integrity (%) at post-thawing were higher (p < .05) with 1.5 mM compared to control. At post-thawing, supravital plasma membrane integrity (%) and viable spermatozoa with intact acrosome (%) were higher with 1.5 compared to 2.0 mM curcumin and control. We concluded that freezability of water buffalo spermatozoa is improved with the addition of 1.5 mM curcumin in extender.

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“…Post-thaw spermatozoa SV-PMI was evaluated as reported by Iqbal et al (2016). Two hundred spermatozoa per sample with unstained heads, and swollen tails (structurally and functionally active membranes) and pink heads, and un-swollen tails (structurally and functionally inactive membranes) were evaluated under phase contrast microscope (magnification, 9400; Leica, Leitz Wetzlar, Germany).…”

Section: Supravital Plasma Membrane Integrity (Sv-pmi %)mentioning

confidence: 99%

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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SUMMARYThe effects of equilibration times (E1, 2 h; E2, 4 h; E3, 6 h), freezing rates (FR1, manual, 5 cm above liquid nitrogen (LN 2 ) for 10 min, plunging in LN 2 ; FR2, programmable ultra-fast, holding at +4°C for 2 min, from 4 to À10°C at À10°C/min, from À10 to À20°C at À15°C/min, from À20 to À120°C at À60°C/min, holding at À120°C for 30 sec, plunging in LN 2 ), and thawing rates (T1, 37°C for 30 sec; T2, 50°C for 15 sec; T3, 70°C for 7 sec) were evaluated on quality of buffalo bull spermatozoa. Progressive motility (%), rapid velocity (%), average path velocity (VAP, lm/s), straight line velocity (VSL, lm/s), and mitochondrial transmembrane potential (%) were higher (p < 0.05) with E2, FR2, and T3 compared to other groups. Sperm curved line velocity (VCL, lm/s) was higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm straightness (%) and linearity (LIN, %) were higher (p < 0.05) with E2 compared to other groups. Sperm LIN was affected (p < 0.05) with T3 compared to other groups. Supravital-plasma membrane integrity (%), viability and acrosome integrity (%) of spermatozoa were higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm DNA integrity (%) was higher (p < 0.05) with FR2 and T1 compared to other groups. We concluded that inclusion of 4 h-equilibration time, programmable ultra-fast freezing rate, and rapid thawing at 70°C for 7 sec in cryopreservation protocol improves the post-thaw quality of buffalo bull spermatozoa.

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Trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in Nili Ravi buffaloes ( Bubalus bubalis )

Cited by 48 publications

References 36 publications

Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress—A pilot study

Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress—A pilot study

Freezability of water buffalo bull (Bubalus bubalis ) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

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