Trehalose and glycogen accumulation is related to the duration of the G₁phase ofSaccharomyces cerevisiae

Abstract

Several factors may control trehalose and glycogen synthesis, like the glucose flux, the growth rate, the intracellular glucose-6-phosphate level and the glucose concentration in the medium. Here, the possible relation of these putative inducers to reserve carbohydrate accumulation was studied under well-defined growth conditions in nitrogen-limited continuous cultures. We showed that the amounts of accumulated trehalose and glycogen were regulated by the growth rate imposed on the culture, whereas other implicated inducers did not exhibit a correlation with reserve carbohydrate accumulation. Trehalose accumulation was induced at a dilution rate (D)</=0.10 h(-1), whereas glycogen accumulation gradually increased at decreasing growth rates. The growth rate dependency of trehalose accumulation was supported by studies in cells overexpressing the G(1)-cyclin CLN3. The trehalose level appeared to be dependent on the duration of the G(1) phase, as trehalose was only accumulated at a G(1) phase duration of more than 5 h in both wild-type and CLN3-overexpressing cells. On the other hand, the glycogen level was reduced by CLN3 overexpression in a cell cycle-independent manner. A possible regulatory mechanism that links trehalose and glycogen accumulation to the growth rate is discussed.

Gaseous environments modify reserve carbohydrate contents and cell survival in the brewing yeast Saccharomyces cerevisiae

“…In this study, intracellular glycogen and trehalose content of Saccharomyces cerevisiae BRAS291 varied between 0.2 and 12.4% of cell dry weight depending on the environmental conditions applied. These values are similar to the data obtained in the literature, i.e., between 0.1 and 25% of dry weight (Lillie and Pringle 1980;Attfield 1997;Mansure et al 1997;Hounsa et al 1998;Parrou et al 1999;Enjalbert et al 2000;Plourde-Owobi et al 2000;Jorgensen et al 2002;Paalman et al 2003;Guillou et al 2004;Samokhvalov et al 2004). The influences of different environmental factors on glycogen and trehalose metabolism in S. cerevisiae as well as their accumulation and mobilization have been studied previously.…”

Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate

“…The trehalose level might even be part of the system that gates entry into S phase of the cell-division cycle (29). The observation that growth on trehalose as the sole carbon source produces spontaneous metabolic cycling in batch cultures (22) may be relevant in this regard, as are the older observations that trehalose is metabolized preferentially during S phase of the cell-division cycle (30,31).…”

MIRACLE: mass isotopomer ratio analysis of U‐13C‐labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites

“…It is known that S. cerevisiae accumulates storage carbohydrates, i.e., glycogen and trehalose under carbon limited conditions. Moreover, the intracellular concentration of storage carbohydrates has been reported to increase with decreasing growth rates (Paalman et al, 2003;Sillje et al, 1999). Under the cultivation conditions we applied, the amount of storage material is f10% of the biomass dry weight.…”