Trehalose acts as a uridine 5′‐diphosphoglucose‐competitive inhibitor of trehalose 6‐phosphate synthase in <i>Corynebacterium glutamicum</i>

Oide, Shinichi; Inui, Masayuki

doi:10.1111/febs.14309

Cited by 8 publications

(4 citation statements)

References 60 publications

(105 reference statements)

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“…The protection might be accomplished by protecting cellular proteins from harmful oxidative attack, as trehalose has been proposed to protect proteins by physicochemical interactions with proteins [37]. Oide and Inui (2017) also recognized the importance of intracellular trehalose accumulation in cells in conferring heat resistance [38]. Furthermore, we found that otsB constitutes an operon with upstream genes NCgl2534 (hypothetical protein), otsA and NCgl2536 (membrane protein), and Pfeifer-Sancar et al also identified these genes as a sub-operon of a longer transcript that includes NCgl2533 ( thrE ) [36].…”

Section: Discussionmentioning

confidence: 99%

Trehalose biosynthetic gene otsB of Corynebacterium glutamicum is regulated by whcE in response to oxidative stress

et al. 2022

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The gene whcE of Corynebacterium glutamicum plays a positive role in oxidative stress responses and the WhcE protein interacts with SpiE. By utilizing 2D-PAGE analysis, we identified the otsB gene to be under the control of whcE. The transcription of otsB, encoding trehalose 6-phosphatase, was stimulated by oxidative stress, and whcE and spiE were involved in diamide-mediated transcriptional stimulation. The ΔotsB strain was created and found to be sensitive to the thiol-specific oxidant diamide, suggesting a role of the gene in stress responses. Genes located upstream of otsB, such as NCgl2534 and otsA, formed an operon and purified WhcE was able to bind to the promoter region of the operon (PNCgl2534), but the binding was only possible in the presence of the oxidant diamide. In addition, the transcriptional activation of PNCgl2534 by WhcE was demonstrated in in vivo assays and the transcription was stimulated in cells exposed to the oxidant diamide. These findings indicate that WhcE is a transcriptional activator, and otsB, which is involved in trehalose biosynthesis, has a role in oxidative stress responses in C. glutamicum .

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Section: Discussionmentioning

confidence: 99%

Trehalose biosynthetic gene otsB of Corynebacterium glutamicum is regulated by whcE in response to oxidative stress

et al. 2022

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“…8). The steP mutants, AD-G, Wild-G, and Wild ΔsteP, are also expected to acquire (partial) thermotolerance by changing the metabolic flux to the PPP: increasing the metabolic flux via G6P to the PPP, probably because of feedback inhibition of OtsA, with trehalose accumulating inside the cells, as seen in other organisms (52,53). In addition, because the steP mutants were found to have some defect in sorbose assimilation, sorbitol seems to be directly utilized instead of sorbose in these cells.…”

Section: Discussionmentioning

confidence: 99%

A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP ⁺ Ratio via Metabolic Change

Matsumoto

Hattori

Matsutani

et al. 2018

Appl Environ Microbiol

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Thermotolerant microorganisms are beneficial to the fermentation industry because they reduce the need for cooling and offer other operational advantages. Previously, we obtained a thermally adapted strain by experimental evolution. In the present study, we found only a single G insertion in the adapted strain, which causes a frameshift in a gene encoding a putative drug transporter. A mutant derivative strain with the single G insertion in the transporter gene (Wild-G) was constructed from the wild-type strain and showed increased thermotolerance. We found that the thermotolerant strains accumulated substantial intracellular trehalose and manifested a defect in sorbose assimilation, suggesting that the transporter is partly involved in trehalose efflux and sorbose uptake and that the defect in the transporter can improve thermotolerance. The Δ strain, constructed by elimination of the trehalose synthesis gene in the wild type, showed no trehalose production but, unexpectedly, much better growth than the adapted strain at high temperatures. The Δ mutant produced more acetate as the final metabolite than the wild-type strain did. We hypothesized that trehalose does not contribute to thermotolerance directly; rather, a metabolic change including increased carbon flux to the pentose phosphate pathway may be the key factor. The NADPH/NADP ratio was higher in strain Wild-G, and much higher in the Δ strain, than in the wild-type strain. Levels of reactive oxygen species (ROS) were lower in the thermotolerant strains. We propose that the defect of the transporter causes the metabolic flux to generate more NADPH, which may enhance thermotolerance in The biorefinery industry has to ensure that microorganisms are robust and retain their viability and function at high temperatures. Here we show that, an industrially important member of the acetic acid bacteria, exhibited enhanced thermotolerance through the reduction of trehalose excretion after thermal adaptation. Although intracellular trehalose may play a key role in thermotolerance, the molecular mechanisms of action of trehalose in thermotolerance are a matter of debate. Our mutated strain that was defective in trehalose synthase genes, producing no trehalose but a larger amount of acetic acid as the end metabolite instead, unexpectedly showed higher thermotolerance than the wild type. Our adapted and mutated thermotolerant strains showed increased NADPH/NADP ratios and reductions in ROS levels. We concluded that in , trehalose does not contribute to thermotolerance directly; rather, the metabolic change increases the NADPH/NADP ratio to enhance thermotolerance.

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“…Trehalose is a nonreducing disaccharide composed of two glucose units linked via an α-α-1,1-glycosidic bond. It is present in various invertebrates such as fungi, bacteria, plants, nematodes, and insects. − Trehalase (TRE), a glucosidase, is the only enzyme that irreversibly hydrolyzes trehalose into two glucose molecules. There are two different forms of TRE in insects: soluble trehalase (TRE1) and membrane-bound trehalase (TRE2), − which work in concert to maintain the insect’s energy supply.…”

Section: Introductionmentioning

confidence: 99%

Impact of Three Thiazolidinone Compounds with Piperine Skeletons on Trehalase Activity and Development of Spodoptera frugiperda Larvae

Tang,

Hu,

Luo

et al. 2024

J. Agric. Food Chem.

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Trehalases (TREs) are pivotal enzymes involved in insect development and reproduction, making them prime targets for pest control. We investigated the inhibitory effect of three thiazolidinones with piperine skeletons (6a, 7b, and 7e) on TRE activity and assessed their impact on the growth and development of the fall armyworm (FAW), Spodoptera frugiperda. The compounds were injected into FAW larvae, while the control group was treated with 2% DMSO solvent. All three compounds effectively inhibited TRE activity, resulting in a significant extension of the pupal development stage. Moreover, the treated larvae exhibited significantly decreased survival rates and a higher incidence of abnormal phenotypes related to growth and development compared to the control group. These results suggest that these TRE inhibitors affect the molting of larvae by regulating the chitin metabolism pathway, ultimately reducing their survival rates. Consequently, these compounds hold potential as environmentally friendly insecticides.

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Trehalose acts as a uridine 5′‐diphosphoglucose‐competitive inhibitor of trehalose 6‐phosphate synthase in Corynebacterium glutamicum

Cited by 8 publications

References 60 publications

Trehalose biosynthetic gene otsB of Corynebacterium glutamicum is regulated by whcE in response to oxidative stress

Trehalose biosynthetic gene otsB of Corynebacterium glutamicum is regulated by whcE in response to oxidative stress

A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP ⁺ Ratio via Metabolic Change

Impact of Three Thiazolidinone Compounds with Piperine Skeletons on Trehalase Activity and Development of Spodoptera frugiperda Larvae

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Trehalose acts as a uridine 5′‐diphosphoglucose‐competitive inhibitor of trehalose 6‐phosphate synthase in Corynebacterium glutamicum

Cited by 8 publications

References 60 publications

Trehalose biosynthetic gene otsB of Corynebacterium glutamicum is regulated by whcE in response to oxidative stress

Trehalose biosynthetic gene otsB of Corynebacterium glutamicum is regulated by whcE in response to oxidative stress

A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP + Ratio via Metabolic Change

Impact of Three Thiazolidinone Compounds with Piperine Skeletons on Trehalase Activity and Development of Spodoptera frugiperda Larvae

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A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP ⁺ Ratio via Metabolic Change