Trehalase fromDictyostelium discoideum: Evaluation of precision and sensitivity of continuous, coupled assays

Killick, Kathleen A.

doi:10.1007/bf02603064

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1980

1984

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Cited by 9 publications

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“…The oxidation product is fluorogenic, and can be estimated in a coupled system for continuous or discontinuous assay of trehalase (514). Eugenol can be replaced by p-hydroxyphenyl acetic acid, and the oxidation product measured fluorimetrically (515 Plant trehalases have not been purified to a great extent; the enzyme from the pollen of Pinus densifora has been purified 81-fold (506). There are no reports of multiple forms of the enzyme; however, it is not certain whether the soluble and insoluble enzymes from various sources are identical.…”

Section: A Occurrence and Localizationmentioning

confidence: 99%