Tree Peony R2R3-MYB Transcription Factor PsMYB30 Promotes Petal Blotch Formation by Activating the Transcription of the Anthocyanin Synthase Gene

Luan, Yuting; Tang, Yuhan; Wang, Xin; Xu, Cong; Tao, Jun; Zhao, Daqiu

doi:10.1093/pcp/pcac085

Cited by 32 publications

(15 citation statements)

References 66 publications

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“…And this result was consistent with the previous finding in the genus Rosa [42]. Compared with Paeonia suffruticosa cultivar 'High Noon' which had both of flavones and flavonols in petals [60] (Supplementary Table S4), Rosa 'Sunset Babylon Eyes' could directly start from the research on flavonol biosynthesis pathway. Thirdly, a variety of different carotenoids played an assisted role in the coloration of petals by adding orange or yellow hue as in other plants' flowers [29,64,65].…”

Section: A Potential Model Plant For Blotch Formation Studysupporting

confidence: 91%

“…However, it is difficult to know about the profile of other flavonoids and other pigments such as carotenoids (Supplementary Table S4). Even if the materials were used with different colors in both blotch and non-blotch parts, there were still few reports comparing anthocyanins, flavones and flavonols, as well as carotenoids at the same time between these two parts [60][61][62][63] (Supplementary Table S4). In this study, the flavonoids and carotenoids were identified and characterized together in a Rosa Hulthemia hybrid 'Sunset Babylon Eyes' with rose-red to dark red blotch and yellow nonblotch parts.…”

Section: A Potential Model Plant For Blotch Formation Studymentioning

confidence: 99%

“…Also, both of DFR and ANS are critical genes for anthocyanin biosynthesis. Despite of the differences, all these three enzyme genes played a vertical role in the molecular mechanism of different blotch color formation in different species [6,9,50,51,60,61,63] (Supplementary Table S4). In 'Sunset Babylon Eyes' , RC7G0055400 (F3'H), RC6G0467567 (DFR), and RC7G0212200 (ANS) which all expressed significantly highly may be the most significant enzyme genes that directly facilitate the pigmentation of blotch.…”

Section: Molecular Mechanism Comparisons Of Blotch Formationmentioning

confidence: 99%

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Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose

Wang

et al. 2023

BMC Plant Biol

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Background Petal blotch is a unique ornamental trait in angiosperm families, and blotch in rose petal is rare and has great esthetic value. However, the cause of the formation of petal blotch in rose is still unclear. The influence of key enzyme genes and regulatory genes in the pigment synthesis pathways needs to be explored and clarified. Results In this study, the rose cultivar ‘Sunset Babylon Eyes’ with rose-red to dark red blotch at the base of petal was selected as the experimental material. The HPLC-DAD and UPLC-TQ-MS analyses indicated that only cyanidin 3,5-O-diglucoside (Cy3G5G) contributed to the blotch pigmentation of ‘Sunset Babylon Eyes’, and the amounts of Cy3G5G varied at different developmental stages. Only flavonols but no flavone were found in blotch and non-blotch parts. As a consequence, kaempferol and its derivatives as well as quercetin and its derivatives may act as background colors during flower developmental stages. Despite of the differences in composition, the total content of carotenoids in blotch and non-blotch parts were similar, and carotenoids may just make the petals show a brighter color. Transcriptomic data, quantitative real-time PCR and promoter sequence analyses indicated that RC7G0058400 (F3’H), RC6G0470600 (DFR) and RC7G0212200 (ANS) may be the key enzyme genes for the early formation and color deepening of blotch at later stages. As for two transcription factor, RC7G0019000 (MYB) and RC1G0363600 (WRKY) may bind to the promoters of critical enzyme genes, or RC1G0363600 (WRKY) may bind to the promoter of RC7G0019000 (MYB) to activate the anthocyanin accumulation in blotch parts of ‘Sunset Babylon Eyes’. Conclusions Our findings provide a theoretical basis for the understanding of the chemical and molecular mechanism for the formation of petal blotch in rose.

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Section: A Potential Model Plant For Blotch Formation Studysupporting

confidence: 91%

Section: A Potential Model Plant For Blotch Formation Studymentioning

confidence: 99%

Section: Molecular Mechanism Comparisons Of Blotch Formationmentioning

confidence: 99%

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Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose

Wang

et al. 2023

BMC Plant Biol

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“…In Vaccinium myrtillus , two MYBA‐type genes, VmMYBA1 and VmMYBA2 , were revealed to regulate anthocyanin biosynthesis in berries and vegetative tissues, while MYBPA1‐type TF was found to promote anthocyanin biosynthesis during berry ripening by directly activating enzyme genes (Karppinen et al, 2021). In Paeonia suffruticosa , PsMYB30 regulates anthocyanin biosynthesis by activating the expression of the PsANS gene to promote petal blotch formation (Luan et al, 2022). In this study, six MYB genes ( RgMYB41 , RgMYB42 , RgMYB43 , RcMYB1 , RcMYB3 , and RhMYB1 ) were cloned from R. glutinosa , R. chingii , and R. henry .…”

Section: Discussionmentioning

confidence: 99%

Purple Rehmannia: Investigation of the activation of R2R3‐MYB transcription factors involved in anthocyanin biosynthesis

Zuo

Miao

et al. 2023

Physiologia Plantarum

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Engineering anthocyanin biosynthesis in herbs could provide health-promoting foods for improving human health. Rehmannia glutinosa is a popular medicinal herb in Asia, and was a health food for the emperors of the Han Dynasty (59 B.C.). In this study, we revealed the differences in anthocyanin composition and content between three Rehmannia species. On the 250, 235 and 206 identified MYBs in the respective species, six could regulate anthocyanin biosynthesis by activating the ANTHOCYANIDIN SYNTHASE (ANS) gene expression. Permanent overexpression of the Rehmannia MYB genes in tobacco strongly promoted anthocyanin content and expression levels of NtANS and other genes. A red appearance of leaves and tuberous/roots was observed, and the total anthocyanin content and the cyanidin-3-O-glucoside content were significantly higher in the lines overexpressing RgMYB41, RgMYB42, and RgMYB43 from R. glutinosa, as well as RcMYB1 and RcMYB3 in R. chingii and RhMYB1 from R. henryi plants. Knocking out of RcMYB3 by CRISPR/Cas9 gene editing resulted in the discoloration of the R. chingii corolla lobes, and decreased the content of anthocyanin. R. glutinosa overexpressing RcMYB3 displayed a distinct purple color in the whole plants, and the antioxidant activity of the transgenic plants was significantly enhanced compared to WT. These results indicate that Rehmannia MYBs can be used to engineer anthocyanin biosynthesis in herbs to improve their additional value, such as increased antioxidant contents.

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“…Except for avonols, there are luteolin glycosides detected in Michelia crassipes [51]. Luteolin and chrysoeriol glycosides had been found in petals of tree peony besides avonols [52]. In both of the blotch and the non-blotch parts, avonols (quercetin glycosides and kaempferol glycosides) existed in petals of 'Sunset Babylon Eyes' at the same time.…”

Section: Flavonols Act As Background Color In Petals Of 'Sunset Babyl...mentioning

confidence: 96%

Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose

Ji¹,

Wang

et al. 2022

Preprint

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Background Petal blotch is a unique ornamental trait in angiosperm families, and blotch in petals of rose is rare and has great esthetic value. Results In this study, the rose cultivar 'Sunset Babylon Eyes' with rose-red to dark red blotch at the base of petals was selected as the experimental material. The HPLC-DAD and UPLC-TQ-MS analyses showed that only cyanidin 3,5-O-diglucoside (Cy3G5G) contributed to the blotch pigmentation of 'Sunset Babylon Eyes', and the amounts of Cy3G5G varied at different developmental stages. Only flavonols but no flavone were found in blotch and non-blotch parts, thereby kaempferol and its derivatives as well as quercetin and its derivatives may act as background color during flower developmental stages. Despite of the differences in composition, the total contents of carotenoids in blotch and non-blotch parts were similar, and carotenoids may just make the petals show a brighter color. Transcriptomic data and quantitative real-time PCR analyses indicated that RC7G0058400 (F3'H), RC6G0470600 (DFR), and RC7G0212200 (ANS) may be the key enzyme genes for the early formation and color deepening of blotch at later stages, and two transcription factor, RC7G0019000 (MYB) and RC1G0363600 (WRKY) may bind to the promoters of critical enzyme genes, or RC1G0363600 (WRKY) may bind to the promoter of RC7G0019000 (MYB) to activate the anthocyanin accumulation in blotch parts of 'Sunset Babylon Eyes'. Conclusions Our findings provide a theoretical basis for the understanding of the chemical and molecular mechanism for the formation of petal blotch in rose.

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Tree Peony R2R3-MYB Transcription Factor PsMYB30 Promotes Petal Blotch Formation by Activating the Transcription of the Anthocyanin Synthase Gene

Cited by 32 publications

References 66 publications

Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose

Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose

Purple Rehmannia: Investigation of the activation of R2R3‐MYB transcription factors involved in anthocyanin biosynthesis

Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose

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