Treatment with Helicobacter pylori-derived VacA attenuates allergic airway disease

Reuter, Sebastian; Raspe, Jonas; Uebner, Hendrik; Contoyannis, Alexandros; Pastille, Eva; Westendorf, Astrid M.; Caso, Georgia C.; Cover, Timothy L.; Müller, Anne; Taube, Christian

doi:10.3389/fimmu.2023.1092801

Cited by 9 publications

(6 citation statements)

References 50 publications

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“…Gastric H. pylori colonization was associated with a shift in the composition of microbial communities in the lung and oral cavity; future studies would be needed to determine if an altered microbiome persists as H. pylori infected animals mature into adulthood. The therapeutic value of H. pyloriderived components for reducing allergic airways inflammation is under investigation; given our present findings it may be of interest to assess whether altering the lung microbiome during infancy could prevent development of childhood asthma 64,65 .…”

Section: Discussionmentioning

confidence: 93%

Helicobacter pylori infection in infant rhesus macaque monkeys is associated with an altered lung and oral microbiome

Siegel,

Jimenez,

Rocha

et al. 2024

Sci Rep

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It is estimated that more than half of the world population has been infected with Helicobacter pylori. Most newly acquired H. pylori infections occur in children before 10 years of age. We hypothesized that early life H. pylori infection could influence the composition of the microbiome at mucosal sites distant to the stomach. To test this hypothesis, we utilized the infant rhesus macaque monkey as an animal model of natural H. pylori colonization to determine the impact of infection on the lung and oral microbiome during a window of postnatal development. From a cohort of 4–7 month-old monkeys, gastric biopsy cultures identified 44% of animals infected by H. pylori. 16S ribosomal RNA gene sequencing of lung washes and buccal swabs from animals showed distinct profiles for the lung and oral microbiome, independent of H. pylori infection. In order of relative abundance, the lung microbiome was dominated by the phyla Proteobacteria, Firmicutes, Bacteroidota, Fusobacteriota, Campilobacterota and Actinobacteriota while the oral microbiome was dominated by Proteobacteria, Firmicutes, Bacteroidota, and Fusobacteriota. In comparison to the oral cavity, the lung was composed of more genera and species that significantly differed by H. pylori status, with a total of 6 genera and species that were increased in H. pylori negative infant monkey lungs. Lung, but not plasma IL-8 concentration was also associated with gastric H. pylori load and lung microbial composition. We found the infant rhesus macaque monkey lung harbors a microbiome signature that is distinct from that of the oral cavity during postnatal development. Gastric H. pylori colonization and IL-8 protein were linked to the composition of microbial communities in the lung and oral cavity. Collectively, these findings provide insight into how H. pylori infection might contribute to the gut-lung axis during early childhood and modulate future respiratory health.

show abstract

Section: Discussionmentioning

confidence: 93%

Helicobacter pylori infection in infant rhesus macaque monkeys is associated with an altered lung and oral microbiome

Siegel,

Jimenez,

Rocha

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Gastric H. pylori colonization was associated with a shift in the composition of microbial communities in the lung and oral cavity; future studies would be needed to determine if an altered microbiome persists as H. pylori infected animals mature into adulthood. The therapeutic value of H. pylori -derived components for reducing allergic airways inflammation is under investigation; given our present findings it may be of interest to assess whether altering the lung microbiome during infancy could prevent development of childhood asthma [ 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori Infection in Infant Rhesus Macaque Monkeys is Associated with an Altered Lung and Oral Microbiome

Siegel

Jimenez

Rocha

et al. 2023

Preprint

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Background It is estimated that more than half of the world population has been infected with Helicobacter pylori. Most newly acquired H. pylori infections occur in children before 10 years of age. We hypothesized that early life H. pylori infection could influence the composition of the microbiome at mucosal sites distant to the stomach. To test this hypothesis, we utilized the infant rhesus macaque monkey as an animal model of natural H. pylori colonization to determine the impact of infection on the lung and oral microbiome during a window of postnatal development. Results From a cohort of 4–7-month-old monkeys, gastric biopsy cultures identified 44% of animals infected by H. pylori. 16S ribosomal RNA gene sequencing of lung washes and buccal swabs from animals showed distinct profiles for the lung and oral microbiome, independent of H. pylori infection. In relative order of abundance, the lung microbiome was dominated by the phyla Proteobacteria, Firmicutes, Bacteroidota, Fusobacteriota, Campilobacterota and Actinobacteriota while the oral microbiome was dominated by Proteobacteria, Firmicutes, Bacteroidota, and Fusobacteriota. Relative to the oral cavity, the lung was composed of more genera and species that significantly differed by H. pylori status, with a total of 6 genera and species that were increased in H. pylori negative infant monkey lungs. Lung, but not plasma IL-8 concentration was also associated with gastric H. pylori load and lung microbial composition. Conclusions We found the infant rhesus macaque monkey lung harbors a microbiome signature that is distinct from that of the oral cavity during postnatal development. Gastric H. pylori colonization and IL-8 protein were linked to the composition of microbial communities in the lung and oral cavity. Collectively, these findings provide insight into how H. pylori infection might contribute to the gut-lung axis during early childhood and modulate future respiratory health.

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“…A large number of observational clinical studies have found that H. pylori infection is associated with a variety of chronic respiratory diseases 2,22 , especially with bronchial asthma 4,5 . At present, the risk effect of H. pylori infection on asthma is still controversial 23,24 , and some studies have suggested that H. pylori infection can increase the incidence and severity of asthma.…”

Section: Discussionmentioning

confidence: 99%

Relationship Between Helicobacter Pylori Infection and Chronic Respiratory Disease: A Two-sample Mendelian Randomization Study

Jia,

Guo,

2023

Preprint

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Background Although many studies have found an association between Helicobacter pylori (H. pylori) infection and chronic respiratory disease, the conclusions are not uniform across studies and the exact relationship remains unclear. Methods We explored the bidirectional causal relationship between H. pylori infection and chronic respiratory disease using a two-sample Mendelian Randomization (MR) method. Data on exposure and outcomes were obtained from the publicly available genome-wide association study (GWAS) database, data on H. pylori infection were obtained from the European Bioinformatics Institute (EBI) database, while chronic respiratory disease from the FinnGen database. The inverse variance weighting (IVW) was used as the primary statistical method. Results MR analyses showed that genetically predicted H. pylori infection had no effect on any of the five chronic respiratory diseases under the Wald ratio and IVW methods. The three MR methods, IVW, MR-Egger, and weighted median (WM), showed that H. pylori infection did not have an effect on any of the four chronic respiratory diseases (asthma, chronic obstructive pulmonary disease (COPD), lung cancer, and bronchiectasis). The IVW method showed that idiopathic pulmonary fibrosis (IPF) was associated with a H. pylori infection risk reduction (OR = 0.97; p = 0.041), which was not significant using the MR-Egger and WM methods. Conclusion Our results found that H. pylori infection had no effect on chronic respiratory diseases, and asthma, COPD, lung cancer, and bronchiectasis have no effect on H. pylori infection, whereas IPF has a mild protective effect against H. pylori infection.

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Treatment with Helicobacter pylori-derived VacA attenuates allergic airway disease

Cited by 9 publications

References 50 publications

Helicobacter pylori infection in infant rhesus macaque monkeys is associated with an altered lung and oral microbiome

Helicobacter pylori infection in infant rhesus macaque monkeys is associated with an altered lung and oral microbiome

Helicobacter pylori Infection in Infant Rhesus Macaque Monkeys is Associated with an Altered Lung and Oral Microbiome

Relationship Between Helicobacter Pylori Infection and Chronic Respiratory Disease: A Two-sample Mendelian Randomization Study

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