Treatment with ginkgo biloba extract protects rats against acute pancreatitis-associated lung injury by modulating alveolar macrophage

Abstract

IntroductionAcute pancreatitis (AP) protease release induces lung parenchymal destruction via inflammatory mediators. Ginkgo biloba has been reported to have anti-inflammatory effects.AimTo evaluate the effect of ginkgo biloba extract on experimental acute pancreatitis-associated lung injury in the rat and to investigate the underlying mechanisms.Material and methodsAcute pancreatitis was induced in rats by injection of 5% sodium taurocholate into the biliary pancreatic duct. Ginkgo biloba extract (GBE) was administered and pancreas and lung injury were assessed by histological examination. Alveolar macrophages were harvested by bronchoalveolar lavage. Specificity fluorescent probe DAF-FM-DA was applied to observe nitric oxide (NO) bioavailability in alveolar macrophage. The expression of tumour necrosis factor α (TNF-α) and macrophage migration inhibitory factor (MIF) protein in alveolar macrophage was studied by ELISA.ResultsIn sodium taurocholate-induced acute pancreatitis, treatment with GBE significantly protected rats against lung injury associated with pancreatitis in histological sections. Ginkgo biloba extract had a tendency to down-regulate NO bioavailability compared with the AP group, but without statistical significance. Moreover, TNF-α and MIF at protein levels in alveolar macrophage with GBE treatment were decreased compared with the AP group.ConclusionsThese results suggest that GBE could effectively protect rats against acute pancreatitis-associated lung injury. The GBE may inhibit excessive activation of alveolar macrophages from acute pancreatitis-associated lung injury through down-regulation of generation of NO, TNF-α and MIF. These findings suggest that ginkgo biloba extract is a suitable candidate as an effective strategy against acute pancreatitis-associated lung injury.

Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats

“…Al-Malki (2015) has reported that cytokines are establish to induce an oxidative stress by the production of oxygen free radicals, inducible nitric oxide synthase (iNOS) expression, NO, which serve as a key cellular mediator of infl ammation and superoxide production. In agreement with the data of other reports, (Biradar and Veeresh 2013;Xu et al 2014), in the current study signifi cant increase in the NO level was noticed in L-arginine received rats. In addition, Salem et al (2014) have reported that the administration of excess L-arginine could induce iNOS activity and raise the NO level in pancreas.…”

IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitisin vitro

“…Although accumulation of activated neutrophils in the lungs is characteristic of ALI, macrophages which reside in the pulmonary interstitium and alveoli are considered to be the most critical cells involved in development of the SAP associated with ALI[8-10]. Macrophages are plastic cells displaying versatile functional phenotypes depending on microenvironments.…”

Ginkgetin aglycone attenuates the apoptosis and inflammation response through nuclear factor‐kB signaling pathway in ischemic‐reperfusion injury

“…Numerous studies have identified the functions and biological features, including lowering blood pressure, dilating blood vessels, improving serum cholesterol. GA can effectively prevent lung injury associated with acute pancreatitis in rats . In a recent study, GA ameliorates LPS‐induced acute kidney injury by activating SIRT1 via inhibiting the NF‐κB signaling pathway .…”