Treatment with disinfectants may induce an increase in viable but non culturable populations of Listeria monocytogenes in biofilms formed in smoked salmon processing environments

Brauge, Thomas; Faille, Christine; Leleu, G.; Denis, Catherine; Hanin, Aurélie; Midelet, Graziella

doi:10.1016/j.fm.2020.103548

Cited by 30 publications

(16 citation statements)

References 20 publications

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“…Though it has been widely used in surface disinfection (Brauge et al 2020;Hayrapetyan et al 2020) and has had attention within drinking water treatments (either combined or standalone use) (Guimarães et al 2014;Karel 2018), hydrogen peroxide is not often contemplated in preoxidation (Xie et al 2016). This encourages exploring its potential, particularly considering it may be an alternative for conditioning source waters to household treatment systems that require turbidity and color to not exceed a certain range, as well as to assess removing DBP precursors.…”

Section: Introductionmentioning

confidence: 99%

Effects of hydrogen peroxide preoxidation on clarification and reduction of the microbial load of groundwater and surface water sources for household treatment

et al. 2021

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Household water treatment (HWT) technologies are a promising strategy for addressing the waterborne diseases burden. However, in order to be efficient, these are often limited to water quality and require it to not exceed a certain threshold of physicochemical and microbiological contamination. Additionally, some popular HWTs, as chlorination, are related to by-product formation. Preoxidation may improve source water quality, and hydrogen peroxide (H2O2) is an oxidant that has not been deeply explored in this specific application, so it could be an innovative approach to HWTs. We investigated effects of H2O2 preoxidation in two natural source waters (surface and groundwater), spiked with a high level of microorganisms. Clarification results suggested this pretreatment may improve life of HWTs. Reduction in microbial load of groundwater was considered ineffective, but 5-min H2O2 preoxidation at 15 mg L−1 led to >4.0 log10 inactivation of Phi X174 coliphage and >3.0 of Escherichia coli in surface water. We believe this performance was increased due to the presence of catalysts in the river water. This raised the point that water quality may be not only impairing, but potentially beneficial to the main HWT and characterization is crucial prior to the implementation of any technologies.

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Section: Introductionmentioning

confidence: 99%

Effects of hydrogen peroxide preoxidation on clarification and reduction of the microbial load of groundwater and surface water sources for household treatment

et al. 2021

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“…Sources of product contamination can come directly from surfaces in the production environment. By directly sampling critical areas offour seafood plants, over eight months, Brauge et al (2020) detected VBNC Lm, primarily after cleaning and disinfection operations (Brauge et al, 2020), highlighting the fact that all industrial disinfectants can fail to inactivate Lm cells on inert surfaces, as discussed below.…”

Section: Induction Of the Vbnc State Of Lm Under Agro-industrial Cond...mentioning

confidence: 98%

“…For example, Brauge et al (2018) showed that Lm mutants lacking N-acetylglucosamine in wall teichoic acids generate a higher population of VBNC in a biofilm (Brauge et al, 2018). The same team thereafter revealed that exposure of Lm biofilms on stainless steel or polyvinyl chloride to quaternary ammonium and hydrogen peroxide disinfectants reduced cultivable populations and induced VBNC populations (Brauge et al, 2020). Similarly, Overney et al (2017) showed induction of the VBNC state in Lm biofilms formed on stainless steel after treatment with a chlorinated alkaline cleaner or quaternary ammonium disinfectant (Overney et al, 2017).…”

Section: Induction Of the Vbnc State Of Lm By Exposure To Antimicrobi...mentioning

confidence: 98%

“…Exposure to light crosslinks the dye to the DNA and blocks the PCR reaction (generally in qPCR). Desneux et al, 2016;Overney et al, 2016;Overney et al, 2017;Brauge et al, 2020;Gu et al, 2020;Truchado et al, 2020;Bernardo et al, 2021 Metataxonomics 16S-rRNA 16S rRNA gene sequencing This method is based on the sequencing of the 16S rRNA gene, which is found in all bacteria and archaea. 16S rRNA gene sequencing is commonly used for identification, classification and quantification of microbes within microbial communities.…”

Section: Overview Of the Vbnc Statementioning

confidence: 99%

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The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum

Lotoux

Milohanic

Bierne

2022

Front. Cell. Infect. Microbiol.

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Many bacterial species, including several pathogens, can enter a so-called “viable but non-culturable” (VBNC) state when subjected to stress. Bacteria in the VBNC state are metabolically active but have lost their ability to grow on standard culture media, which compromises their detection by conventional techniques based on bacterial division. Under certain conditions, VBNC bacteria can regain their growth capacity and, for pathogens, their virulence potential, through a process called resuscitation. Here, we review the current state of knowledge of the VBNC state of Listeria monocytogenes (Lm), a Gram-positive pathogenic bacterium responsible for listeriosis, one of the most dangerous foodborne zoonosis. After a brief summary of characteristics of VBNC bacteria, we highlight work on VBNC Lm in the environment and in agricultural and food industry settings, with particular emphasis on the impact of antimicrobial treatments. We subsequently discuss recent data suggesting that Lm can enter the VBNC state in the host, raising the possibility that VBNC forms contribute to the asymptomatic carriage of this pathogen in wildlife, livestock and even humans. We also consider the resuscitation and virulence potential of VBNC Lm and the danger posed by these bacteria to at-risk individuals, particularly pregnant women. Overall, we put forth the hypothesis that VBNC forms contribute to adaptation, persistence, and transmission of Lm between different ecological niches in the One-Health continuum, and suggest that screening for healthy carriers, using alternative techniques to culture-based enrichment methods, should better prevent listeriosis risks.

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“…Another related concern is the ability of the resistant populations to form biofilms in food-processing areas. This includes biofilms formed by the spore-forming bacteria, which are very resistant to disinfectants and mild to moderate thermal wash [ 4 ], and biofilms from non-spore-forming bacteria, which eventually develop resistance to the treatment and can lead to severe food poisoning and spoilage issues, beyond their role in transmitting antimicrobial resistance (AMR) in the community [ 5 ]. Nevertheless, a limited number of disinfectants can be used within food-processing areas, as they might not only leave residues in the final products but can also damage the surfaces of equipment, making it further challenging to reduce bacterial contamination in the final product [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Nature-Inspired Antimicrobial Surfaces and Their Potential Applications in Food Industries

Soni

Brightwell

2022

Foods

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Antimicrobial resistance (AMR) is a growing global concern and has called for the integration of different areas of expertise for designing robust solutions. One such approach is the development of antimicrobial surfaces to combat the emerging resistance in microbes against drugs and disinfectants. This review is a compressive summary of the work done in the field of material science, chemistry, and microbiology in the development of antimicrobial materials and surfaces that are inspired by examples in nature. The focus includes examples of natural antimicrobial surfaces, such as cicada wings or nanopillars, dragonfly wings, shrimp shells, taro leaves, lotus leaves, sharkskin, gecko skin, and butterfly wings, along with their mechanism of action. Techniques, compositions, and combinations that have been developed to synthetically mimic these surfaces against bacterial/viral and fungal growth in food-processing areas have also been discussed. The applications of synthetic mimics of natural antimicrobial surfaces in food-processing environments is still a naïve area of research. However, this review highlights the potential applications of natural antimicrobial surfaces in the food-processing environment as well as outlines the challenges that need mitigations.

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Treatment with disinfectants may induce an increase in viable but non culturable populations of Listeria monocytogenes in biofilms formed in smoked salmon processing environments

Cited by 30 publications

References 20 publications

Effects of hydrogen peroxide preoxidation on clarification and reduction of the microbial load of groundwater and surface water sources for household treatment

Effects of hydrogen peroxide preoxidation on clarification and reduction of the microbial load of groundwater and surface water sources for household treatment

The Viable But Non-Culturable State of Listeria monocytogenes in the One-Health Continuum

Nature-Inspired Antimicrobial Surfaces and Their Potential Applications in Food Industries

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