Treatment of vibriosis in European sea bass larvae, Dicentrarchus labrax L., with oxolinic acid administered by bath or through medicated nauplii of Artemia franciscana (Kellogg): efficacy and residual kinetics

Abstract

European sea bass larvae were challenged by bath with Listonella anguillarum strain 332A, 2.5×10(7) CFUmL(-1) for 1h. Fish either received no treatment or oral treatment with Artemia franciscana (Kellog) nauplii enriched with oxolinic acid, or bath treatments with oxolinic acid. Medication commenced 1day following challenge and was performed on days 1, 3 and 5 post-challenge at a dosage of 20mgL(-1) for 2h for bath treatments, while two doses each of 750 nauplii per fish were administered daily for five consecutive days in oral treatments. Cumulative mortality reached 96% for the unmedicated challenged group, 32% in the group receiving bath treatments and 17% in the group receiving medicated nauplii. Pharmacokinetic parameters of oxolinic acid were calculated in sea bass larvae, for both treatments. Steady-state concentrations of oxolinic acid of 48.0 and 75.2μgg(-1) were achieved for bath treatment and oral treatment, respectively, while the elimination half-life was calculated to be 25.1h for bath treatment and 21.7h for oral treatment.

Modulation of Innate Immune-Related Genes and Glucocorticoid Synthesis in Gnotobiotic Full-Sibling European Sea Bass (Dicentrarchus labrax) Larvae Challenged With Vibrio anguillarum

“…Vibriosis, an infectious disease whose etiological agent is V. anguillarum , has been considered one of the most important mariculture fish diseases ( 52 ). Several studies have reported the susceptibility of sea bass to V. anguillarum ( 53 , 54 ). In our results, no significant variations were observed in the cumulative survival in non-challenged groups along the study.…”

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

“…Industrial production of sea bass is still hampered by low and unpredictable survival of larvae and juvenile fish due to disease outbreaks such as infections caused by Vibrio spp. (46,47). Vibriosis is a bottleneck for the expansion of sustainable sea bass aquaculture and causes high mortality especially during the larviculture.…”

Development of enriched Artemia and Moina in larviculture of fish and crustaceans: a review

“…The first-line defense against potential pathogens is regulated by immunoglobulin; meanwhile, fish skin mucus possesses various innate immune factors, including lysozymes, glycoproteins, and proteases (Shephard 1993, Magnadóttir 2006. The evaluation of enriched Artemia using probiotics Bacillus subtilis and Lactobacillus plantarum against vibriosis (Vibriosis anguillarum) in European sea bass (Dicentrarchus labrax) larvae was carried out by Touraki et al (2012). The study proved that the most efficient protection with the highest survival rates against vibriosis in fish larvae was offered by Artemia enriched with B. subtilis, up to 86.7% compared to the untreated larvae group (36.7%).…”