Treatment of triclosan through enhanced microbial biodegradation

Balakrishnan, P.; Mohan, S.

doi:10.1016/j.jhazmat.2021.126430

Cited by 26 publications

(10 citation statements)

References 54 publications

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“…Treatment with LacT resulted in a decrease in the concentration of DHBP and TCS, indicated by the reduction in the absorbance at 262 and 280 nm, respectively (Figures a and a). The addition of LacT resulted in the removal of ∼8% of DHBP and ∼37% of TCS in 1 h. Previously, the bioremediation of TCS with the Providencia rettgeri MB-IIT strain exhibiting laccase and manganese peroxidase activity has been reported . The isolated strain degraded ∼98% TCS in 24 h. The bioconversion of DS and DHBP with bacterial laccase has not been outlined in the literature.…”

Section: Resultsmentioning

confidence: 99%

Thermostable Bacterial Laccase: Catalytic Properties and Its Application in Biotransformation of Emerging Pollutants

Panwar,

Lzaod,

Dutta

2023

ACS Omega

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Laccases have been predominantly reported in fungi, and primarily, fungal laccases are currently exploited in industrial applications. However, extremophilic bacterial laccases possess immense potential, as they can withstand extreme temperatures, pH, and salt concentrations. In addition, unlike fungal laccases, the production of bacterial laccases is cost-effective. Therefore, bacterial laccases are gaining significant attention for their large-scale applications. Previously, we reported a novel thermostable laccase (LacT) from Brevibacillus agri . Herein, we have confirmed that LacT shares a high sequence similarity with CotA laccase from Bacillus amyloliquefaciens . Peptide mass fingerprinting of LacT was conducted via matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF/MS-MS). Inductively coupled plasma-optical emission spectroscopic (ICP-OES) analysis revealed the presence of ∼3.95 copper ions per protein molecule. Moreover, the secondary and tertiary structure of LacT was studied using circular dichroism (CD) and fluorescence spectroscopy. The absence of notable shifts in CD and fluorescence spectra with an increase in temperature established that LacT remains intact even at elevated temperatures. Analysis of the thermal denaturation profile of LacT by thermogravimetric analysis (TGA) also confirmed its temperature stability. Thereafter, we exploited LacT in its application for the bioremediation of phenolic endocrine disruptors, namely, triclosan, 4,4′-dihydroxybiphenyl, and dienestrol. LacT oxidizes 4,4′-dihydroxybiphenyl and triclosan but no LacT activity was detected with dienestrol. The rate of biotransformation of 4,4′-dihydroxybiphenyl and triclosan increased in the presence of CuSO 4 and a redox mediator, ABTS. Transformation of dienestrol was observed only with LacT in the presence of ABTS. This study establishes the application of LacT for the bioremediation of phenolic compounds.

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Section: Resultsmentioning

confidence: 99%

Thermostable Bacterial Laccase: Catalytic Properties and Its Application in Biotransformation of Emerging Pollutants

Panwar,

Lzaod,

Dutta

2023

ACS Omega

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“…Recently, a novel biological degradation method for the removal of TCS in municipal wastewater has been proposed, which includes the identification of degrading bacteria from municipal wastewater sludge. It was found that bacterial strain belonging to Providencia rettgeri group, namely P. rettgeri MB-IIT strain, could be advantageously used to degrade TCS that was present in the wastewater [ 251 ]. A recent study on the plant Glyceria maxima showed that the amounts of TCS in plant shoots were 1.4–2.5 times higher than that in roots [ 252 ].…”

Section: Removal From Aquatic Environment/degradation Techniquesmentioning

confidence: 99%

Triclosan: A Small Molecule with Controversial Roles

et al. 2022

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Triclosan (TCS), a broad-spectrum antimicrobial agent, has been widely used in personal care products, medical products, plastic cutting boards, and food storage containers. Colgate Total® toothpaste, containing 10 mM TCS, is effective in controlling biofilm formation and maintaining gingival health. Given its broad usage, TCS is present ubiquitously in the environment. Given its strong lipophilicity and accumulation ability in organisms, it is potentially harmful to biohealth. Several reports suggest the toxicity of this compound, which is inserted in the class of endocrine disrupting chemicals (EDCs). In September 2016, TCS was banned by the U.S. Food and Drug Administration (FDA) and the European Union in soap products. Despite these problems, its application in personal care products within certain limits is still allowed. Today, it is still unclear whether TCS is truly toxic to mammals and the adverse effects of continuous, long-term, and low concentration exposure remain unknown. Indeed, some recent reports suggest the use of TCS as a repositioned drug for cancer treatment and cutaneous leishmaniasis. In this scenario it is necessary to investigate the advantages and disadvantages of TCS, to understand whether its use is advisable or not. This review intends to highlight the pros and cons that are associated with the use of TCS in humans.

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“…Besides, the detection of the release of chlorine ions by ion chromatography and the detection of intermediate metabolites indicates that dechlorination occurs during TCS degradation, but the mechanisms involved are not clear (Figure 3C pathway a) [79]. In addition, in 2021, Balakrishnan and Mohan [18] used LC-MS/MS to detect the intermediates of TCS degradation by Providencia rettgeri MB-IIT, and found that methylation (+CH 3 ) and complete mineralization were the two possible degradation pathways of the strain MB-IIT. The hydroxyl of TCS was initially biomethylated, and then transformed into downstream intermediates through combined dechlorination [18].…”

Section: Metabolic Pathways Of Tcs-degrading Microorganismsmentioning

confidence: 99%

“…However, it is difficult to remove TCS in the actual treatment process due to its easy production of intermediates and high maintenance cost [15][16][17]. Studies have shown that TCS could be degraded by the ammonia-oxidizing bacteria (AOB) and heterotrophic bacteria in the water environment, and using microbial metabolism to degrade TCS in wastewater can lead to complete mineralization of TCS without producing toxic intermediate metabolites [1,18]. At present, with the great progress of isolation and culture technology, more and more TCS-degrading microorganisms and TCS-degrading microbial consortia have been obtained in the laboratory, and their degrading characteristics have been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Degradation of Triclosan in the Water Environment by Microorganisms: A Review

Yin

Jiang³

et al. 2022

Microorganisms

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Triclosan (TCS), a kind of pharmaceuticals and personal care products (PPCPs), is widely used and has had a large production over years. It is an emerging pollutant in the water environment that has attracted global attention due to its toxic effects on organisms and aquatic ecosystems, and its concentrations in the water environment are expected to increase since the COVID-19 pandemic outbreak. Some researchers found that microbial degradation of TCS is an environmentally sustainable technique that results in the mineralization of large amounts of organic pollutants without toxic by-products. In this review, we focus on the fate of TCS in the water environment, the diversity of TCS-degrading microorganisms, biodegradation pathways and molecular mechanisms, in order to provide a reference for the efficient degradation of TCS and other PPCPs by microorganisms.

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Treatment of triclosan through enhanced microbial biodegradation

Cited by 26 publications

References 54 publications

Thermostable Bacterial Laccase: Catalytic Properties and Its Application in Biotransformation of Emerging Pollutants

Thermostable Bacterial Laccase: Catalytic Properties and Its Application in Biotransformation of Emerging Pollutants

Triclosan: A Small Molecule with Controversial Roles

Degradation of Triclosan in the Water Environment by Microorganisms: A Review

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