Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole

Abstract

Tetracycline antibiotics are widely used in human and veterinary medicine; however, residual amounts of these antibiotics in the environment are of concern since they could contribute to selection of resistant bacteria. In this study, tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) and oxytetracycline (OTC) were treated with laccase from the white rot fungus Trametes versicolor in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). High performance liquid chromatography demonstrated that DC and CTC were completely eliminated after 15 min, while TC and CTC were eliminated after 1 h. This system also resulted in a complete loss of inhibition of growth of Escherichia coli and Bacillus subtilis and the green alga Pseudokirchneriella subcapitata with decreasing tetracycline antibiotic concentration. These results suggest that the laccase-HBT system is effective in eliminating tetracycline antibiotics and removing their ecotoxicity.

Enzyme Initiated Radical Polymerizations

“…Likewise halophenols [178][179][180][181][182] bisphenol A, [183][184][185] various hormones and so-called endocrine disrupting compounds [186][187][188] or even TNT [189,190] have been reported. Non-phenolic compounds may be ‗inactivated' using the LMS or PMS strategy [36,[191][192][193][194][195][196][197][198].…”

Erythromycin degradation by esterase (EreB) in enzymatic membrane reactors

“…Enzyme activity was stable over 100 hours of reaction and the average degradation speed was 1.19 mg h -1 . As observed in previous studies [16], covalent grafting could protect the biocatalyst from potential leaching or conformational changes, leaving the active sites available to react with the substrate. Nevertheless, the erythromycin depletion potential of this EMR is low; after 24h, only 20% of erythromycin was degraded.…”

“…Erythromycin content was measured by high-performance liquid chromatography coupled to triple quadrupole mass spectrometry (HPLC-MS/MS) as in our previous studies [16]. Samples were injected through an Onyx® C18 column (25 mm x 4.6 mm) with a Waters e2695 Separation Module, and the 576.7 m/z transition was detected with a Micromass Quattro micro API device.…”

Fungal Bioremediation of Emerging Micropollutants in Municipal Wastewaters

“…The presence of ABTS enhanced transformation of dymron (Maruyama et al 2006), TCS (Kim and Nicell 2006a), sulfonamide 14 antibiotics (Weng et al 2013), BPA Nicell 2006b, Macellaro et al 2014) and parabens analogues (Macellaro et al 2014). On the other side HBT was found to be fundamental during laccase treatments of carbamazepine , iso-butylparaben and n-butylparaben (Mizuno et al 2009), anti-inflammatories and estrogens (Lloret et al 2010) and tetracycline antibiotics (Suda et al 2012). …”

“…Inhibition of R. subcapitata growth (UNI EN ISO 8692: 2005 method) was followed in the presence of tetracycline antibiotics (Suda et al 2012). YES assay was used to evaluate the estrogenic activity of parabens (Mizuno et al 2009) and estrogens in municipal wastewaters (Auriol et al 2008).…”